Excretory/secretory products of Angiostrongylus cantonensis fifth-stage larvae induce endoplasmic reticulum stress via the Sonic hedgehog pathway in mouse astrocytes
Abstract Background Angiostrongylus cantonensis is an important food-borne zoonotic parasite. Humans are non-permissive hosts, and this parasite develops into fifth-stage larvae (L5) in the brain and subarachnoid cavity and then induces eosinophilic meningitis and eosinophilic meningoencephalitis. E...
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| Format: | Article |
| Language: | English |
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BMC
2020-06-01
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| Series: | Parasites & Vectors |
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| Online Access: | http://link.springer.com/article/10.1186/s13071-020-04189-w |
| _version_ | 1818159487490457600 |
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| author | Kuang-Yao Chen Yi-Ju Chen Chien-Ju Cheng Kai-Yuan Jhan Lian-Chen Wang |
| author_facet | Kuang-Yao Chen Yi-Ju Chen Chien-Ju Cheng Kai-Yuan Jhan Lian-Chen Wang |
| author_sort | Kuang-Yao Chen |
| collection | DOAJ |
| description | Abstract Background Angiostrongylus cantonensis is an important food-borne zoonotic parasite. Humans are non-permissive hosts, and this parasite develops into fifth-stage larvae (L5) in the brain and subarachnoid cavity and then induces eosinophilic meningitis and eosinophilic meningoencephalitis. Excretory/secretory products (ESPs) are valuable targets for the investigation of host-parasite interactions. These products contain a wide range of molecules for penetrating defensive barriers and avoiding the immune response of the host. Endoplasmic reticulum (ER) stress has been found to be associated with a wide range of parasitic infections and inflammation. ER stress can increase cell survival via the activation of downstream signalling. However, the mechanisms of ER stress in A. cantonensis infection have not yet been clarified. This study was designed to investigate the molecular mechanisms of ER stress in astrocytes after treatment with the ESPs of A. cantonensis L5. Results The results demonstrated that A. cantonensis infection activated astrocytes in the mouse hippocampus and induced the expression of ER stress-related molecules. Next, the data showed that the expression of ER stress-related molecules and the Ca2+ concentration were significantly increased in activated astrocytes after treatment with the ESPs of L5 of A. cantonensis. Ultimately, we found that ESPs induced GRP78 expression via the Sonic hedgehog (Shh) signalling pathway. Conclusions These findings suggest that in astrocytes, the ESPs of A. cantonensis L5 induce ER stress and that the Shh signalling pathway plays an important role in this process. |
| first_indexed | 2024-12-11T15:46:46Z |
| format | Article |
| id | doaj.art-62fad6f167304d41a311a0dad8d7ef55 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-11T15:46:46Z |
| publishDate | 2020-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-62fad6f167304d41a311a0dad8d7ef552022-12-22T00:59:41ZengBMCParasites & Vectors1756-33052020-06-0113111110.1186/s13071-020-04189-wExcretory/secretory products of Angiostrongylus cantonensis fifth-stage larvae induce endoplasmic reticulum stress via the Sonic hedgehog pathway in mouse astrocytesKuang-Yao Chen0Yi-Ju Chen1Chien-Ju Cheng2Kai-Yuan Jhan3Lian-Chen Wang4Department of Parasitology, School of Medicine, China Medical UniversityDepartment of Parasitology, School of Medicine, China Medical UniversityDepartment of Parasitology, College of Medicine, Chang Gung UniversityGraduate Institute of Biomedical Sciences, College of Medicine, Chang Gung UniversityDepartment of Parasitology, College of Medicine, Chang Gung UniversityAbstract Background Angiostrongylus cantonensis is an important food-borne zoonotic parasite. Humans are non-permissive hosts, and this parasite develops into fifth-stage larvae (L5) in the brain and subarachnoid cavity and then induces eosinophilic meningitis and eosinophilic meningoencephalitis. Excretory/secretory products (ESPs) are valuable targets for the investigation of host-parasite interactions. These products contain a wide range of molecules for penetrating defensive barriers and avoiding the immune response of the host. Endoplasmic reticulum (ER) stress has been found to be associated with a wide range of parasitic infections and inflammation. ER stress can increase cell survival via the activation of downstream signalling. However, the mechanisms of ER stress in A. cantonensis infection have not yet been clarified. This study was designed to investigate the molecular mechanisms of ER stress in astrocytes after treatment with the ESPs of A. cantonensis L5. Results The results demonstrated that A. cantonensis infection activated astrocytes in the mouse hippocampus and induced the expression of ER stress-related molecules. Next, the data showed that the expression of ER stress-related molecules and the Ca2+ concentration were significantly increased in activated astrocytes after treatment with the ESPs of L5 of A. cantonensis. Ultimately, we found that ESPs induced GRP78 expression via the Sonic hedgehog (Shh) signalling pathway. Conclusions These findings suggest that in astrocytes, the ESPs of A. cantonensis L5 induce ER stress and that the Shh signalling pathway plays an important role in this process.http://link.springer.com/article/10.1186/s13071-020-04189-wAngiostrongylus cantonensisFifth-stage larvaeExcretory/secretory productsAstrocytesEndoplasmic reticulum stressSonic hedgehog pathway |
| spellingShingle | Kuang-Yao Chen Yi-Ju Chen Chien-Ju Cheng Kai-Yuan Jhan Lian-Chen Wang Excretory/secretory products of Angiostrongylus cantonensis fifth-stage larvae induce endoplasmic reticulum stress via the Sonic hedgehog pathway in mouse astrocytes Parasites & Vectors Angiostrongylus cantonensis Fifth-stage larvae Excretory/secretory products Astrocytes Endoplasmic reticulum stress Sonic hedgehog pathway |
| title | Excretory/secretory products of Angiostrongylus cantonensis fifth-stage larvae induce endoplasmic reticulum stress via the Sonic hedgehog pathway in mouse astrocytes |
| title_full | Excretory/secretory products of Angiostrongylus cantonensis fifth-stage larvae induce endoplasmic reticulum stress via the Sonic hedgehog pathway in mouse astrocytes |
| title_fullStr | Excretory/secretory products of Angiostrongylus cantonensis fifth-stage larvae induce endoplasmic reticulum stress via the Sonic hedgehog pathway in mouse astrocytes |
| title_full_unstemmed | Excretory/secretory products of Angiostrongylus cantonensis fifth-stage larvae induce endoplasmic reticulum stress via the Sonic hedgehog pathway in mouse astrocytes |
| title_short | Excretory/secretory products of Angiostrongylus cantonensis fifth-stage larvae induce endoplasmic reticulum stress via the Sonic hedgehog pathway in mouse astrocytes |
| title_sort | excretory secretory products of angiostrongylus cantonensis fifth stage larvae induce endoplasmic reticulum stress via the sonic hedgehog pathway in mouse astrocytes |
| topic | Angiostrongylus cantonensis Fifth-stage larvae Excretory/secretory products Astrocytes Endoplasmic reticulum stress Sonic hedgehog pathway |
| url | http://link.springer.com/article/10.1186/s13071-020-04189-w |
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