Activity and Function in Human Cells of the Evolutionary Conserved Exonuclease Polynucleotide Phosphorylase
Polynucleotide phosphorylase (PNPase) is a phosphorolytic RNA exonuclease highly conserved throughout evolution. Human PNPase (hPNPase) is located in mitochondria and is essential for mitochondrial function and homeostasis. Not surprisingly, mutations in the <i>PNPT1</i> gene, encoding h...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/3/1652 |
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| author | Federica A. Falchi Roberto Pizzoccheri Federica Briani |
| author_facet | Federica A. Falchi Roberto Pizzoccheri Federica Briani |
| author_sort | Federica A. Falchi |
| collection | DOAJ |
| description | Polynucleotide phosphorylase (PNPase) is a phosphorolytic RNA exonuclease highly conserved throughout evolution. Human PNPase (hPNPase) is located in mitochondria and is essential for mitochondrial function and homeostasis. Not surprisingly, mutations in the <i>PNPT1</i> gene, encoding hPNPase, cause serious diseases. hPNPase has been implicated in a plethora of processes taking place in different cell compartments and involving other proteins, some of which physically interact with hPNPase. This paper reviews hPNPase RNA binding and catalytic activity in relation with the protein structure and in comparison, with the activity of bacterial PNPases. The functions ascribed to hPNPase in different cell compartments are discussed, highlighting the gaps that still need to be filled to understand the physiological role of this ancient protein in human cells. |
| first_indexed | 2024-03-09T23:45:46Z |
| format | Article |
| id | doaj.art-62fc5e0057034be1858ba3d9254b6358 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:45:46Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-62fc5e0057034be1858ba3d9254b63582023-11-23T16:44:06ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-01-01233165210.3390/ijms23031652Activity and Function in Human Cells of the Evolutionary Conserved Exonuclease Polynucleotide PhosphorylaseFederica A. Falchi0Roberto Pizzoccheri1Federica Briani2Dipartimento di Bioscienze, Università degli Studi di Milano, 20133 Milano, ItalyDipartimento di Bioscienze, Università degli Studi di Milano, 20133 Milano, ItalyDipartimento di Bioscienze, Università degli Studi di Milano, 20133 Milano, ItalyPolynucleotide phosphorylase (PNPase) is a phosphorolytic RNA exonuclease highly conserved throughout evolution. Human PNPase (hPNPase) is located in mitochondria and is essential for mitochondrial function and homeostasis. Not surprisingly, mutations in the <i>PNPT1</i> gene, encoding hPNPase, cause serious diseases. hPNPase has been implicated in a plethora of processes taking place in different cell compartments and involving other proteins, some of which physically interact with hPNPase. This paper reviews hPNPase RNA binding and catalytic activity in relation with the protein structure and in comparison, with the activity of bacterial PNPases. The functions ascribed to hPNPase in different cell compartments are discussed, highlighting the gaps that still need to be filled to understand the physiological role of this ancient protein in human cells.https://www.mdpi.com/1422-0067/23/3/1652polyribonucleotide phosphorylasePNPase<i>PNPT1</i>RNA decayRNA stabilityRNA binding protein |
| spellingShingle | Federica A. Falchi Roberto Pizzoccheri Federica Briani Activity and Function in Human Cells of the Evolutionary Conserved Exonuclease Polynucleotide Phosphorylase International Journal of Molecular Sciences polyribonucleotide phosphorylase PNPase <i>PNPT1</i> RNA decay RNA stability RNA binding protein |
| title | Activity and Function in Human Cells of the Evolutionary Conserved Exonuclease Polynucleotide Phosphorylase |
| title_full | Activity and Function in Human Cells of the Evolutionary Conserved Exonuclease Polynucleotide Phosphorylase |
| title_fullStr | Activity and Function in Human Cells of the Evolutionary Conserved Exonuclease Polynucleotide Phosphorylase |
| title_full_unstemmed | Activity and Function in Human Cells of the Evolutionary Conserved Exonuclease Polynucleotide Phosphorylase |
| title_short | Activity and Function in Human Cells of the Evolutionary Conserved Exonuclease Polynucleotide Phosphorylase |
| title_sort | activity and function in human cells of the evolutionary conserved exonuclease polynucleotide phosphorylase |
| topic | polyribonucleotide phosphorylase PNPase <i>PNPT1</i> RNA decay RNA stability RNA binding protein |
| url | https://www.mdpi.com/1422-0067/23/3/1652 |
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