Preparation data of the bromodomains BRD3(1), BRD3(2), BRD4(1), and BRPF1B and crystallization of BRD4(1)-inhibitor complexes
This article presents detailed purification procedures for the bromodomains BRD3(1), BRD3(2), BRD4(1), and BRPF1B. In addition we provide crystallization protocols for apo BRD4(1) and BRD4(1) in complex with numerous inhibitors. The protocols described here were successfully applied to obtain affini...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-06-01
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| Series: | Data in Brief |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340916302232 |
| _version_ | 1818448771769434112 |
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| author | Martin Hügle Xavier Lucas Gerhard Weitzel Dmytro Ostrovskyi Bernhard Breit Stefan Gerhardt Karin Schmidtkunz Manfred Jung Roland Schüle Oliver Einsle Stefan Günther Daniel Wohlwend |
| author_facet | Martin Hügle Xavier Lucas Gerhard Weitzel Dmytro Ostrovskyi Bernhard Breit Stefan Gerhardt Karin Schmidtkunz Manfred Jung Roland Schüle Oliver Einsle Stefan Günther Daniel Wohlwend |
| author_sort | Martin Hügle |
| collection | DOAJ |
| description | This article presents detailed purification procedures for the bromodomains BRD3(1), BRD3(2), BRD4(1), and BRPF1B. In addition we provide crystallization protocols for apo BRD4(1) and BRD4(1) in complex with numerous inhibitors. The protocols described here were successfully applied to obtain affinity data by isothermal titration calorimetry (ITC) and by differential scanning fluorimetry (DSF) as well as structural characterizations of BRD4(1) inhibitor complexes (PDB codes: PDB: 4LYI, PDB: 4LZS, PDB: 4LYW, PDB: 4LZR, PDB: 4LYS, PDB: 5D24, PDB: 5D25, PDB: 5D26, PDB: 5D3H, PDB: 5D3J, PDB: 5D3L, PDB: 5D3N, PDB: 5D3P, PDB: 5D3R, PDB: 5D3S, PDB: 5D3T). These data have been reported previously and are discussed in more detail elsewhere [1,2]. Keywords: Epigenetics, Bromodomains, Drug discovery, X-ray crystallography |
| first_indexed | 2024-12-14T20:24:49Z |
| format | Article |
| id | doaj.art-6301727c76704813bd8737a83f4fc8fd |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-12-14T20:24:49Z |
| publishDate | 2016-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-6301727c76704813bd8737a83f4fc8fd2022-12-21T22:48:38ZengElsevierData in Brief2352-34092016-06-01713701374Preparation data of the bromodomains BRD3(1), BRD3(2), BRD4(1), and BRPF1B and crystallization of BRD4(1)-inhibitor complexesMartin Hügle0Xavier Lucas1Gerhard Weitzel2Dmytro Ostrovskyi3Bernhard Breit4Stefan Gerhardt5Karin Schmidtkunz6Manfred Jung7Roland Schüle8Oliver Einsle9Stefan Günther10Daniel Wohlwend11Albert-Ludwigs-Universität Freiburg, Institut für Biochemie, Albertstr. 21, D-79104 Freiburg, GermanyCollege of Life Sciences, Division of Biological Chemistry and Drug Discovery, University of Dundee, James Black Centre, Dow Street, Dundee DD1 5EH, United KingdomAlbert-Ludwigs-Universität Freiburg, Institut für Organische Chemie, Albertstr. 21, D-79104 Freiburg, GermanyAlbert-Ludwigs-Universität Freiburg, Institut für Organische Chemie, Albertstr. 21, D-79104 Freiburg, GermanyAlbert-Ludwigs-Universität Freiburg, Institut für Organische Chemie, Albertstr. 21, D-79104 Freiburg, GermanyAlbert-Ludwigs-Universität Freiburg, Institut für Biochemie, Albertstr. 21, D-79104 Freiburg, GermanyAlbert-Ludwigs-Universität Freiburg, Institut für Pharmazeutische Wissenschaften, Albertstr. 25, D-79104 Freiburg, GermanyAlbert-Ludwigs-Universität Freiburg, Institut für Pharmazeutische Wissenschaften, Albertstr. 25, D-79104 Freiburg, GermanyUrologische Klinik/Frauenklinik, Klinikum der Universität Freiburg and BIOSS Centre for Biological Signalling Studies, Freiburg, GermanyAlbert-Ludwigs-Universität Freiburg, Institut für Biochemie, Albertstr. 21, D-79104 Freiburg, GermanyAlbert-Ludwigs-Universität Freiburg, Institut für Pharmazeutische Wissenschaften, Hermann-Herder-Str. 8, D-79104 Freiburg, GermanyAlbert-Ludwigs-Universität Freiburg, Institut für Biochemie, Albertstr. 21, D-79104 Freiburg, Germany; Corresponding author.This article presents detailed purification procedures for the bromodomains BRD3(1), BRD3(2), BRD4(1), and BRPF1B. In addition we provide crystallization protocols for apo BRD4(1) and BRD4(1) in complex with numerous inhibitors. The protocols described here were successfully applied to obtain affinity data by isothermal titration calorimetry (ITC) and by differential scanning fluorimetry (DSF) as well as structural characterizations of BRD4(1) inhibitor complexes (PDB codes: PDB: 4LYI, PDB: 4LZS, PDB: 4LYW, PDB: 4LZR, PDB: 4LYS, PDB: 5D24, PDB: 5D25, PDB: 5D26, PDB: 5D3H, PDB: 5D3J, PDB: 5D3L, PDB: 5D3N, PDB: 5D3P, PDB: 5D3R, PDB: 5D3S, PDB: 5D3T). These data have been reported previously and are discussed in more detail elsewhere [1,2]. Keywords: Epigenetics, Bromodomains, Drug discovery, X-ray crystallographyhttp://www.sciencedirect.com/science/article/pii/S2352340916302232 |
| spellingShingle | Martin Hügle Xavier Lucas Gerhard Weitzel Dmytro Ostrovskyi Bernhard Breit Stefan Gerhardt Karin Schmidtkunz Manfred Jung Roland Schüle Oliver Einsle Stefan Günther Daniel Wohlwend Preparation data of the bromodomains BRD3(1), BRD3(2), BRD4(1), and BRPF1B and crystallization of BRD4(1)-inhibitor complexes Data in Brief |
| title | Preparation data of the bromodomains BRD3(1), BRD3(2), BRD4(1), and BRPF1B and crystallization of BRD4(1)-inhibitor complexes |
| title_full | Preparation data of the bromodomains BRD3(1), BRD3(2), BRD4(1), and BRPF1B and crystallization of BRD4(1)-inhibitor complexes |
| title_fullStr | Preparation data of the bromodomains BRD3(1), BRD3(2), BRD4(1), and BRPF1B and crystallization of BRD4(1)-inhibitor complexes |
| title_full_unstemmed | Preparation data of the bromodomains BRD3(1), BRD3(2), BRD4(1), and BRPF1B and crystallization of BRD4(1)-inhibitor complexes |
| title_short | Preparation data of the bromodomains BRD3(1), BRD3(2), BRD4(1), and BRPF1B and crystallization of BRD4(1)-inhibitor complexes |
| title_sort | preparation data of the bromodomains brd3 1 brd3 2 brd4 1 and brpf1b and crystallization of brd4 1 inhibitor complexes |
| url | http://www.sciencedirect.com/science/article/pii/S2352340916302232 |
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