Recombinant Bovine Herpesvirus Type I Expressing the Bovine Viral Diarrhea Virus E2 Protein Could Effectively Prevent Infection by Two Viruses
Bovine respiratory disease complex (BRDC) is a comprehensive disease in cattle caused by various viral and bacterial infections. Among them, bovine herpesvirus type I (BoHV−1) and bovine viral diarrhea virus (BVDV) play important roles and have caused huge financial losses for the cattle industry wo...
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| Format: | Article |
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MDPI AG
2022-07-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/8/1618 |
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| author | Chun-Yu Liu Hao Guo Hong-Zhe Zhao Li-Na Hou Yong-Jun Wen Feng-Xue Wang |
| author_facet | Chun-Yu Liu Hao Guo Hong-Zhe Zhao Li-Na Hou Yong-Jun Wen Feng-Xue Wang |
| author_sort | Chun-Yu Liu |
| collection | DOAJ |
| description | Bovine respiratory disease complex (BRDC) is a comprehensive disease in cattle caused by various viral and bacterial infections. Among them, bovine herpesvirus type I (BoHV−1) and bovine viral diarrhea virus (BVDV) play important roles and have caused huge financial losses for the cattle industry worldwide. At present, vaccines against BRDC include trivalent attenuated BoHV−1, BVDV−1, and BVDV−2 live vaccines, BoHV−1 live attenuated vaccines, and BoHV−1/BVDV bivalent live attenuated vaccines, which have limitations in terms of their safety and efficacy. To solve these problems, we optimized the codon of the BVDV−1 E2 gene, added the signal peptide sequence of the BoHV−1 gD gene, expressed double BVDV−1 E2 glycoproteins in tandem at the BoHV−1 gE gene site, and constructed a BoHV−1 genetics-engineered vectored vaccine with gE gene deletion, named BoHV−1 gE/E2−Linker−E2<sup>+</sup> and BoHV−1 ΔgE. This study compared the protective effects in BoHV−1, BoHV−1 ΔgE, BoHV−1 gE/E2−Linker−E2<sup>+</sup>, and BVDV−1 inactivated antigen immunized guinea pigs and calves. The results showed that BoHV−1 gE/E2−Linker−E2<sup>+</sup> could successfully induce guinea pigs and calves to produce specific neutralizing antibodies against BVDV−1. In addition, after BoHV−1 and BVDV−1 challenges, BoHV−1 gE/E2−Linker−E2<sup>+</sup> can produce a specific neutralizing antibody response against BoHV−1 and BVDV−1 infections. Calves immunized with this type of virus can be distinguished as either vaccinated animals (gE<sup>-</sup>) or naturally infected animals (gE<sup>+</sup>). In summary, our data suggest that BoHV−1 gE/E2−Linker−E2<sup>+</sup> and BoHV−1 ΔgE have great potential to prevent BVDV−1 or BoHV−1 infection. |
| first_indexed | 2024-03-09T12:22:46Z |
| format | Article |
| id | doaj.art-6301f5d636534c28be5aea315882a323 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T12:22:46Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-6301f5d636534c28be5aea315882a3232023-11-30T22:38:17ZengMDPI AGViruses1999-49152022-07-01148161810.3390/v14081618Recombinant Bovine Herpesvirus Type I Expressing the Bovine Viral Diarrhea Virus E2 Protein Could Effectively Prevent Infection by Two VirusesChun-Yu Liu0Hao Guo1Hong-Zhe Zhao2Li-Na Hou3Yong-Jun Wen4Feng-Xue Wang5College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, ChinaBovine respiratory disease complex (BRDC) is a comprehensive disease in cattle caused by various viral and bacterial infections. Among them, bovine herpesvirus type I (BoHV−1) and bovine viral diarrhea virus (BVDV) play important roles and have caused huge financial losses for the cattle industry worldwide. At present, vaccines against BRDC include trivalent attenuated BoHV−1, BVDV−1, and BVDV−2 live vaccines, BoHV−1 live attenuated vaccines, and BoHV−1/BVDV bivalent live attenuated vaccines, which have limitations in terms of their safety and efficacy. To solve these problems, we optimized the codon of the BVDV−1 E2 gene, added the signal peptide sequence of the BoHV−1 gD gene, expressed double BVDV−1 E2 glycoproteins in tandem at the BoHV−1 gE gene site, and constructed a BoHV−1 genetics-engineered vectored vaccine with gE gene deletion, named BoHV−1 gE/E2−Linker−E2<sup>+</sup> and BoHV−1 ΔgE. This study compared the protective effects in BoHV−1, BoHV−1 ΔgE, BoHV−1 gE/E2−Linker−E2<sup>+</sup>, and BVDV−1 inactivated antigen immunized guinea pigs and calves. The results showed that BoHV−1 gE/E2−Linker−E2<sup>+</sup> could successfully induce guinea pigs and calves to produce specific neutralizing antibodies against BVDV−1. In addition, after BoHV−1 and BVDV−1 challenges, BoHV−1 gE/E2−Linker−E2<sup>+</sup> can produce a specific neutralizing antibody response against BoHV−1 and BVDV−1 infections. Calves immunized with this type of virus can be distinguished as either vaccinated animals (gE<sup>-</sup>) or naturally infected animals (gE<sup>+</sup>). In summary, our data suggest that BoHV−1 gE/E2−Linker−E2<sup>+</sup> and BoHV−1 ΔgE have great potential to prevent BVDV−1 or BoHV−1 infection.https://www.mdpi.com/1999-4915/14/8/1618BoHV−1BVDV−1BoHV−1 gE/E2−Linker−E2<sup>+</sup>BoHV−1 ΔgEE2 glycoproteinsignal peptide |
| spellingShingle | Chun-Yu Liu Hao Guo Hong-Zhe Zhao Li-Na Hou Yong-Jun Wen Feng-Xue Wang Recombinant Bovine Herpesvirus Type I Expressing the Bovine Viral Diarrhea Virus E2 Protein Could Effectively Prevent Infection by Two Viruses Viruses BoHV−1 BVDV−1 BoHV−1 gE/E2−Linker−E2<sup>+</sup> BoHV−1 ΔgE E2 glycoprotein signal peptide |
| title | Recombinant Bovine Herpesvirus Type I Expressing the Bovine Viral Diarrhea Virus E2 Protein Could Effectively Prevent Infection by Two Viruses |
| title_full | Recombinant Bovine Herpesvirus Type I Expressing the Bovine Viral Diarrhea Virus E2 Protein Could Effectively Prevent Infection by Two Viruses |
| title_fullStr | Recombinant Bovine Herpesvirus Type I Expressing the Bovine Viral Diarrhea Virus E2 Protein Could Effectively Prevent Infection by Two Viruses |
| title_full_unstemmed | Recombinant Bovine Herpesvirus Type I Expressing the Bovine Viral Diarrhea Virus E2 Protein Could Effectively Prevent Infection by Two Viruses |
| title_short | Recombinant Bovine Herpesvirus Type I Expressing the Bovine Viral Diarrhea Virus E2 Protein Could Effectively Prevent Infection by Two Viruses |
| title_sort | recombinant bovine herpesvirus type i expressing the bovine viral diarrhea virus e2 protein could effectively prevent infection by two viruses |
| topic | BoHV−1 BVDV−1 BoHV−1 gE/E2−Linker−E2<sup>+</sup> BoHV−1 ΔgE E2 glycoprotein signal peptide |
| url | https://www.mdpi.com/1999-4915/14/8/1618 |
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