ANTIOXIDANT ACTIVITY AND CYTOTOXIC EFFECT OF CHILEAN Buddleja globosa (MATICO) AND Ribes magellanicum (ZARZAPARRILLA) FLOWER EXTRACTS
The native Chilean shrubs Buddleja globosa (matico) and Ribes magellanicum (zarzaparrilla) are used widely at a rural level, due to their medicinal properties. Nevertheless, little is known about their secondary metabolites and cytotoxic effect. The aim of this study was to evaluate the content of d...
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| Format: | Article |
| Language: | English |
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University of Life Sciences in Lublin - Publishing House
2020-12-01
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| Series: | Acta Scientiarum Polonorum: Hortorum Cultus |
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| Online Access: | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/2653 |
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| author | Pedro Zamorano-Aguilar Marcela Morales Yudrum Rivillas Juan López Benjamín A. Rojano |
| author_facet | Pedro Zamorano-Aguilar Marcela Morales Yudrum Rivillas Juan López Benjamín A. Rojano |
| author_sort | Pedro Zamorano-Aguilar |
| collection | DOAJ |
| description | The native Chilean shrubs Buddleja globosa (matico) and Ribes magellanicum (zarzaparrilla) are used widely at a rural level, due to their medicinal properties. Nevertheless, little is known about their secondary metabolites and cytotoxic effect. The aim of this study was to evaluate the content of different compounds like catechin, epicatechin, p-coumaric acid and the antioxidant capacity by ABTS, ORAC, FRAP and DPPH methods. In addition, the cytotoxic activity of both extracts was evaluated against Chinese hamster ovary (CHO-K1) cell lines by MTT and neutral red assays. The results suggest that the most abundant constituent in Budleja globosa and Ribes magellanicum were catechin (682.43 mg/100 g DW) and epicatechin (3362.08 mg/100 g DW) respectively; while the ORAC methodology showed an elevated antioxidant activity for matico (134147.31 μmol Trolox Eq/100 g DW). On the other hand, both extracts at the assayed concentrations affect the membrane stability and cellular metabolic capacity of the CHO-K1 cell lines. These finding provide a direction for further researches, and suggest the matico and zarzaparrilla flower extracts as promising sources of antioxidants, and as research objects through the analyze of their metabolic behavior and antitumoral potential.
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| first_indexed | 2024-03-09T00:17:15Z |
| format | Article |
| id | doaj.art-6307d7f8eaad4c2291fab20dd6c07be3 |
| institution | Directory Open Access Journal |
| issn | 1644-0692 2545-1405 |
| language | English |
| last_indexed | 2024-03-09T00:17:15Z |
| publishDate | 2020-12-01 |
| publisher | University of Life Sciences in Lublin - Publishing House |
| record_format | Article |
| series | Acta Scientiarum Polonorum: Hortorum Cultus |
| spelling | doaj.art-6307d7f8eaad4c2291fab20dd6c07be32023-12-12T07:51:23ZengUniversity of Life Sciences in Lublin - Publishing HouseActa Scientiarum Polonorum: Hortorum Cultus1644-06922545-14052020-12-0119610.24326/asphc.2020.6.5ANTIOXIDANT ACTIVITY AND CYTOTOXIC EFFECT OF CHILEAN Buddleja globosa (MATICO) AND Ribes magellanicum (ZARZAPARRILLA) FLOWER EXTRACTSPedro Zamorano-Aguilar0Marcela Morales1Yudrum Rivillas2Juan López3Benjamín A. Rojano4Graduate School, Faculty of Agricultural Science, Universidad Austral de Chile, Av. Carlos Ibañez del Campo S/N Valdivia, ChileGrupo Química de los Productos Naturales y los Alimentos, Facultad de Ciencias, Universidad Nacional de Colombia, Calle 59a N° 63-20 Medellín, ColombiaGrupo Biotecnología Animal, Facultad de Ciencias, Universidad Nacional de Colombia, Calle 59a N° 63-20 Medellín, ColombiaGrupo Biotecnología Animal, Facultad de Ciencias, Universidad Nacional de Colombia, Calle 59a N° 63-20 Medellín, ColombiaGrupo Química de los Productos Naturales y los Alimentos, Facultad de Ciencias, Universidad Nacional de Colombia, Calle 59a N° 63-20 Medellín, ColombiaThe native Chilean shrubs Buddleja globosa (matico) and Ribes magellanicum (zarzaparrilla) are used widely at a rural level, due to their medicinal properties. Nevertheless, little is known about their secondary metabolites and cytotoxic effect. The aim of this study was to evaluate the content of different compounds like catechin, epicatechin, p-coumaric acid and the antioxidant capacity by ABTS, ORAC, FRAP and DPPH methods. In addition, the cytotoxic activity of both extracts was evaluated against Chinese hamster ovary (CHO-K1) cell lines by MTT and neutral red assays. The results suggest that the most abundant constituent in Budleja globosa and Ribes magellanicum were catechin (682.43 mg/100 g DW) and epicatechin (3362.08 mg/100 g DW) respectively; while the ORAC methodology showed an elevated antioxidant activity for matico (134147.31 μmol Trolox Eq/100 g DW). On the other hand, both extracts at the assayed concentrations affect the membrane stability and cellular metabolic capacity of the CHO-K1 cell lines. These finding provide a direction for further researches, and suggest the matico and zarzaparrilla flower extracts as promising sources of antioxidants, and as research objects through the analyze of their metabolic behavior and antitumoral potential. https://czasopisma.up.lublin.pl/index.php/asphc/article/view/2653maticozarzaparrillaantioxidant capacitycytotoxic activitybioactive compoundspolyphenols |
| spellingShingle | Pedro Zamorano-Aguilar Marcela Morales Yudrum Rivillas Juan López Benjamín A. Rojano ANTIOXIDANT ACTIVITY AND CYTOTOXIC EFFECT OF CHILEAN Buddleja globosa (MATICO) AND Ribes magellanicum (ZARZAPARRILLA) FLOWER EXTRACTS Acta Scientiarum Polonorum: Hortorum Cultus matico zarzaparrilla antioxidant capacity cytotoxic activity bioactive compounds polyphenols |
| title | ANTIOXIDANT ACTIVITY AND CYTOTOXIC EFFECT OF CHILEAN Buddleja globosa (MATICO) AND Ribes magellanicum (ZARZAPARRILLA) FLOWER EXTRACTS |
| title_full | ANTIOXIDANT ACTIVITY AND CYTOTOXIC EFFECT OF CHILEAN Buddleja globosa (MATICO) AND Ribes magellanicum (ZARZAPARRILLA) FLOWER EXTRACTS |
| title_fullStr | ANTIOXIDANT ACTIVITY AND CYTOTOXIC EFFECT OF CHILEAN Buddleja globosa (MATICO) AND Ribes magellanicum (ZARZAPARRILLA) FLOWER EXTRACTS |
| title_full_unstemmed | ANTIOXIDANT ACTIVITY AND CYTOTOXIC EFFECT OF CHILEAN Buddleja globosa (MATICO) AND Ribes magellanicum (ZARZAPARRILLA) FLOWER EXTRACTS |
| title_short | ANTIOXIDANT ACTIVITY AND CYTOTOXIC EFFECT OF CHILEAN Buddleja globosa (MATICO) AND Ribes magellanicum (ZARZAPARRILLA) FLOWER EXTRACTS |
| title_sort | antioxidant activity and cytotoxic effect of chilean buddleja globosa matico and ribes magellanicum zarzaparrilla flower extracts |
| topic | matico zarzaparrilla antioxidant capacity cytotoxic activity bioactive compounds polyphenols |
| url | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/2653 |
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