Auxotrophic Mutant Strains of Rhizobium etli Reveal New Nodule Development Phenotypes
We report here the isolation and characterization of amino acid-requiring mutant strains of Rhizobium etli. We observe that the phenotype of most mutations, even when causing a strict auxotrophy, is overcome by cross-feeding from the host plant Phaseolus vulgaris, thereby allowing bacterial producti...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2002-05-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2002.15.5.501 |
| _version_ | 1811291263455985664 |
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| author | Simona Ferraioli Rosarita Tatè Michele Cermola Renée Favre Maurizio Iaccarino Eduardo J. Patriarca |
| author_facet | Simona Ferraioli Rosarita Tatè Michele Cermola Renée Favre Maurizio Iaccarino Eduardo J. Patriarca |
| author_sort | Simona Ferraioli |
| collection | DOAJ |
| description | We report here the isolation and characterization of amino acid-requiring mutant strains of Rhizobium etli. We observe that the phenotype of most mutations, even when causing a strict auxotrophy, is overcome by cross-feeding from the host plant Phaseolus vulgaris, thereby allowing bacterial production of Nod factors and, consequently, nodule induction. Conversely, light and electron microscopy analysis reveals that the nodules induced by all mutants, including those with normal external morphology, are halted or strongly altered at intermediate or late stages of development. Moreover, some mutants induce nodules that display novel symbiotic phenotypes, such as specific alterations of the invaded cells or the presence of a reduced number of abnormally shaped uninvaded cells. Other mutants induce nodules showing an early and vast necrosis of the central tissue, a phenotype not previously observed in bean nodules, not even in nodules induced by a Fix¯ mutant. These observations indicate that amino acid auxotrophs represent a powerful tool to study the development of globose determinate-type nodules and emphasize the importance of establishing their histology and citology before considerations of metabolic exchange are made. |
| first_indexed | 2024-04-13T04:26:49Z |
| format | Article |
| id | doaj.art-630ec204e6e64a39baf290f685ceb15c |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T04:26:49Z |
| publishDate | 2002-05-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-630ec204e6e64a39baf290f685ceb15c2022-12-22T03:02:28ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062002-05-0115550151010.1094/MPMI.2002.15.5.501Auxotrophic Mutant Strains of Rhizobium etli Reveal New Nodule Development PhenotypesSimona FerraioliRosarita TatèMichele CermolaRenée FavreMaurizio IaccarinoEduardo J. PatriarcaWe report here the isolation and characterization of amino acid-requiring mutant strains of Rhizobium etli. We observe that the phenotype of most mutations, even when causing a strict auxotrophy, is overcome by cross-feeding from the host plant Phaseolus vulgaris, thereby allowing bacterial production of Nod factors and, consequently, nodule induction. Conversely, light and electron microscopy analysis reveals that the nodules induced by all mutants, including those with normal external morphology, are halted or strongly altered at intermediate or late stages of development. Moreover, some mutants induce nodules that display novel symbiotic phenotypes, such as specific alterations of the invaded cells or the presence of a reduced number of abnormally shaped uninvaded cells. Other mutants induce nodules showing an early and vast necrosis of the central tissue, a phenotype not previously observed in bean nodules, not even in nodules induced by a Fix¯ mutant. These observations indicate that amino acid auxotrophs represent a powerful tool to study the development of globose determinate-type nodules and emphasize the importance of establishing their histology and citology before considerations of metabolic exchange are made.https://apsjournals.apsnet.org/doi/10.1094/MPMI.2002.15.5.501cell wallroot exudatesymbiosissymbiosome differentiation |
| spellingShingle | Simona Ferraioli Rosarita Tatè Michele Cermola Renée Favre Maurizio Iaccarino Eduardo J. Patriarca Auxotrophic Mutant Strains of Rhizobium etli Reveal New Nodule Development Phenotypes Molecular Plant-Microbe Interactions cell wall root exudate symbiosis symbiosome differentiation |
| title | Auxotrophic Mutant Strains of Rhizobium etli Reveal New Nodule Development Phenotypes |
| title_full | Auxotrophic Mutant Strains of Rhizobium etli Reveal New Nodule Development Phenotypes |
| title_fullStr | Auxotrophic Mutant Strains of Rhizobium etli Reveal New Nodule Development Phenotypes |
| title_full_unstemmed | Auxotrophic Mutant Strains of Rhizobium etli Reveal New Nodule Development Phenotypes |
| title_short | Auxotrophic Mutant Strains of Rhizobium etli Reveal New Nodule Development Phenotypes |
| title_sort | auxotrophic mutant strains of rhizobium etli reveal new nodule development phenotypes |
| topic | cell wall root exudate symbiosis symbiosome differentiation |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2002.15.5.501 |
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