Vigorous Growing of Donor Plantlets by Liquid Overlay in Subcultures Is the Key to Cryopreservation of Endangered Species <i>Pogostemon yatabeanus</i>
Cryopreservation is a unique option for the long-term conservation of threatened plant species with non-orthodox or limitedly available seeds. However, the wide application of cryopreservation for the protection of wild flora is hampered by some reasons: limits of source material available, difficul...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/22/3127 |
| _version_ | 1797464154507837440 |
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| author | Hyoeun Lee Haenghoon Kim |
| author_facet | Hyoeun Lee Haenghoon Kim |
| author_sort | Hyoeun Lee |
| collection | DOAJ |
| description | Cryopreservation is a unique option for the long-term conservation of threatened plant species with non-orthodox or limitedly available seeds. However, the wide application of cryopreservation for the protection of wild flora is hampered by some reasons: limits of source material available, difficulties in in vitro propagation, needs to re-optimize protocol steps for new species, etc. In this study, using an endemic and endangered Korean species, <i>Pogostemon yatabeanus</i>, we investigated subculture medium and supplements on in vitro growth of donor plants: medium strength, gelling agents, liquid overlay, plant hormones, and activated charcoal. Subculture conditions of each cycle tested significantly impacted on height and dry weight of subcultured donor plantlets. Among the treatments tested, the overlay of the liquid medium on top of gellan gum-gelled medium significantly increased the growth of shoots and roots. In the droplet-vitrification procedure, the survival and regeneration of cryopreserved shoot tips were critically impacted by the dry weight of donor plantlets (CORELL = 0.85~0.95) which was affected by the following subculture conditions. Moreover, every subsequent subculture cycle before cryopreservation positively or negatively impacted post-cryopreservation regeneration. This study highlights the vigor of donor plantlets for post-cryopreservation regeneration and provides practices for the revitalization of donor plants during subcultures. |
| first_indexed | 2024-03-09T18:03:57Z |
| format | Article |
| id | doaj.art-630ec857b0bf4c24852307543399498e |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T18:03:57Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-630ec857b0bf4c24852307543399498e2023-11-24T09:40:03ZengMDPI AGPlants2223-77472022-11-011122312710.3390/plants11223127Vigorous Growing of Donor Plantlets by Liquid Overlay in Subcultures Is the Key to Cryopreservation of Endangered Species <i>Pogostemon yatabeanus</i>Hyoeun Lee0Haenghoon Kim1Department of Agricultural Life Science, Sunchon National University, Suncheon 57922, Republic of KoreaDepartment of Agricultural Life Science, Sunchon National University, Suncheon 57922, Republic of KoreaCryopreservation is a unique option for the long-term conservation of threatened plant species with non-orthodox or limitedly available seeds. However, the wide application of cryopreservation for the protection of wild flora is hampered by some reasons: limits of source material available, difficulties in in vitro propagation, needs to re-optimize protocol steps for new species, etc. In this study, using an endemic and endangered Korean species, <i>Pogostemon yatabeanus</i>, we investigated subculture medium and supplements on in vitro growth of donor plants: medium strength, gelling agents, liquid overlay, plant hormones, and activated charcoal. Subculture conditions of each cycle tested significantly impacted on height and dry weight of subcultured donor plantlets. Among the treatments tested, the overlay of the liquid medium on top of gellan gum-gelled medium significantly increased the growth of shoots and roots. In the droplet-vitrification procedure, the survival and regeneration of cryopreserved shoot tips were critically impacted by the dry weight of donor plantlets (CORELL = 0.85~0.95) which was affected by the following subculture conditions. Moreover, every subsequent subculture cycle before cryopreservation positively or negatively impacted post-cryopreservation regeneration. This study highlights the vigor of donor plantlets for post-cryopreservation regeneration and provides practices for the revitalization of donor plants during subcultures.https://www.mdpi.com/2223-7747/11/22/3127activated charcoalalternative vitrification solutionsgelling agentregrowth mediumsubculture medium |
| spellingShingle | Hyoeun Lee Haenghoon Kim Vigorous Growing of Donor Plantlets by Liquid Overlay in Subcultures Is the Key to Cryopreservation of Endangered Species <i>Pogostemon yatabeanus</i> Plants activated charcoal alternative vitrification solutions gelling agent regrowth medium subculture medium |
| title | Vigorous Growing of Donor Plantlets by Liquid Overlay in Subcultures Is the Key to Cryopreservation of Endangered Species <i>Pogostemon yatabeanus</i> |
| title_full | Vigorous Growing of Donor Plantlets by Liquid Overlay in Subcultures Is the Key to Cryopreservation of Endangered Species <i>Pogostemon yatabeanus</i> |
| title_fullStr | Vigorous Growing of Donor Plantlets by Liquid Overlay in Subcultures Is the Key to Cryopreservation of Endangered Species <i>Pogostemon yatabeanus</i> |
| title_full_unstemmed | Vigorous Growing of Donor Plantlets by Liquid Overlay in Subcultures Is the Key to Cryopreservation of Endangered Species <i>Pogostemon yatabeanus</i> |
| title_short | Vigorous Growing of Donor Plantlets by Liquid Overlay in Subcultures Is the Key to Cryopreservation of Endangered Species <i>Pogostemon yatabeanus</i> |
| title_sort | vigorous growing of donor plantlets by liquid overlay in subcultures is the key to cryopreservation of endangered species i pogostemon yatabeanus i |
| topic | activated charcoal alternative vitrification solutions gelling agent regrowth medium subculture medium |
| url | https://www.mdpi.com/2223-7747/11/22/3127 |
| work_keys_str_mv | AT hyoeunlee vigorousgrowingofdonorplantletsbyliquidoverlayinsubculturesisthekeytocryopreservationofendangeredspeciesipogostemonyatabeanusi AT haenghoonkim vigorousgrowingofdonorplantletsbyliquidoverlayinsubculturesisthekeytocryopreservationofendangeredspeciesipogostemonyatabeanusi |