Longitudinal Study of the Effects of Flammulina velutipes Stipe Wastes on the Cecal Microbiota of Laying Hens
ABSTRACT Because antibiotics have been phased out of use in poultry feed, measures to improve intestinal health have been sought. Dietary fiber may be beneficial to intestinal health by modulating gut microbial composition, but the exact changes it induces remain unclear. In this study, we evaluated...
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2023-02-01
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| Series: | mSystems |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/msystems.00835-22 |
| _version_ | 1797895838594236416 |
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| author | Jiali Wei Huanwei Xiao Yingbo Wei Ivan Stève Nguepi Tsopmejio Chang Sun Haoyuan Wu Zhouyu Jin Hui Song |
| author_facet | Jiali Wei Huanwei Xiao Yingbo Wei Ivan Stève Nguepi Tsopmejio Chang Sun Haoyuan Wu Zhouyu Jin Hui Song |
| author_sort | Jiali Wei |
| collection | DOAJ |
| description | ABSTRACT Because antibiotics have been phased out of use in poultry feed, measures to improve intestinal health have been sought. Dietary fiber may be beneficial to intestinal health by modulating gut microbial composition, but the exact changes it induces remain unclear. In this study, we evaluated the effect of Flammulina velutipes stipe wastes (FVW) on the cecal microbiotas of laying chickens at ages spanning birth to 490 days. Using clonal sequencing and 16S rRNA high-throughput sequencing, we showed that FVW improved the microbial diversity when they under fluctuated. The evolvement of the microbiota enhanced the physiological development of laying hens. Supplementation of FVW enriched the relative abundance of Sutterella, Ruminiclostridium, Synergistes, Anaerostipes, and Rikenellaceae, strengthened the positive connection between Firmicutes and Bacteroidetes, and increased the concentration of short-chain fatty acids (SCFAs) in early life. FVW maintains gut microbiota homeostasis by regulating Th1, Th2, and Th17 balance and secretory IgA (S-IgA) level. In conclusion, we showed that FVW induces microbial changes that are potentially beneficial for intestinal immunity. IMPORTANCE Dietary fiber is popularly used in poultry farming to improve host health and metabolism. Microbial composition is known to be influenced by dietary fiber use, although the exact FVW-induced changes remain unclear. This study provided a first comparison of the effects of FVW and the most commonly used antibiotic growth promoter (flavomycin) on the cecal microbiotas of laying hens from birth to 490 days of age. We found that supplementation with FVW altered cecal microbial composition, thereby affecting the correlation network between members of the microbiota, and subsequently affecting the intestinal immune homeostasis. |
| first_indexed | 2024-04-10T07:32:04Z |
| format | Article |
| id | doaj.art-63160c2be8844788abf19e700598c928 |
| institution | Directory Open Access Journal |
| issn | 2379-5077 |
| language | English |
| last_indexed | 2024-04-10T07:32:04Z |
| publishDate | 2023-02-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mSystems |
| spelling | doaj.art-63160c2be8844788abf19e700598c9282023-02-23T14:01:38ZengAmerican Society for MicrobiologymSystems2379-50772023-02-018110.1128/msystems.00835-22Longitudinal Study of the Effects of Flammulina velutipes Stipe Wastes on the Cecal Microbiota of Laying HensJiali Wei0Huanwei Xiao1Yingbo Wei2Ivan Stève Nguepi Tsopmejio3Chang Sun4Haoyuan Wu5Zhouyu Jin6Hui Song7School of Life Science, Jilin Agricultural University, Changchun, Jilin, People’s Republic of ChinaSchool of Life Science, Jilin Agricultural University, Changchun, Jilin, People’s Republic of ChinaSchool of Life Science, Jilin Agricultural University, Changchun, Jilin, People’s Republic of ChinaSchool of Life Science, Jilin Agricultural University, Changchun, Jilin, People’s Republic of ChinaSchool of Life Science, Jilin Agricultural University, Changchun, Jilin, People’s Republic of ChinaSchool of Life Science, Jilin Agricultural University, Changchun, Jilin, People’s Republic of ChinaSchool of Life Science, Jilin Agricultural University, Changchun, Jilin, People’s Republic of ChinaSchool of Life Science, Jilin Agricultural University, Changchun, Jilin, People’s Republic of ChinaABSTRACT Because antibiotics have been phased out of use in poultry feed, measures to improve intestinal health have been sought. Dietary fiber may be beneficial to intestinal health by modulating gut microbial composition, but the exact changes it induces remain unclear. In this study, we evaluated the effect of Flammulina velutipes stipe wastes (FVW) on the cecal microbiotas of laying chickens at ages spanning birth to 490 days. Using clonal sequencing and 16S rRNA high-throughput sequencing, we showed that FVW improved the microbial diversity when they under fluctuated. The evolvement of the microbiota enhanced the physiological development of laying hens. Supplementation of FVW enriched the relative abundance of Sutterella, Ruminiclostridium, Synergistes, Anaerostipes, and Rikenellaceae, strengthened the positive connection between Firmicutes and Bacteroidetes, and increased the concentration of short-chain fatty acids (SCFAs) in early life. FVW maintains gut microbiota homeostasis by regulating Th1, Th2, and Th17 balance and secretory IgA (S-IgA) level. In conclusion, we showed that FVW induces microbial changes that are potentially beneficial for intestinal immunity. IMPORTANCE Dietary fiber is popularly used in poultry farming to improve host health and metabolism. Microbial composition is known to be influenced by dietary fiber use, although the exact FVW-induced changes remain unclear. This study provided a first comparison of the effects of FVW and the most commonly used antibiotic growth promoter (flavomycin) on the cecal microbiotas of laying hens from birth to 490 days of age. We found that supplementation with FVW altered cecal microbial composition, thereby affecting the correlation network between members of the microbiota, and subsequently affecting the intestinal immune homeostasis.https://journals.asm.org/doi/10.1128/msystems.00835-22dietary fiberFlammulina velutipesflavomycinmicrobiotalaying hens |
| spellingShingle | Jiali Wei Huanwei Xiao Yingbo Wei Ivan Stève Nguepi Tsopmejio Chang Sun Haoyuan Wu Zhouyu Jin Hui Song Longitudinal Study of the Effects of Flammulina velutipes Stipe Wastes on the Cecal Microbiota of Laying Hens mSystems dietary fiber Flammulina velutipes flavomycin microbiota laying hens |
| title | Longitudinal Study of the Effects of Flammulina velutipes Stipe Wastes on the Cecal Microbiota of Laying Hens |
| title_full | Longitudinal Study of the Effects of Flammulina velutipes Stipe Wastes on the Cecal Microbiota of Laying Hens |
| title_fullStr | Longitudinal Study of the Effects of Flammulina velutipes Stipe Wastes on the Cecal Microbiota of Laying Hens |
| title_full_unstemmed | Longitudinal Study of the Effects of Flammulina velutipes Stipe Wastes on the Cecal Microbiota of Laying Hens |
| title_short | Longitudinal Study of the Effects of Flammulina velutipes Stipe Wastes on the Cecal Microbiota of Laying Hens |
| title_sort | longitudinal study of the effects of flammulina velutipes stipe wastes on the cecal microbiota of laying hens |
| topic | dietary fiber Flammulina velutipes flavomycin microbiota laying hens |
| url | https://journals.asm.org/doi/10.1128/msystems.00835-22 |
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