In vitro and in vivo evaluation of anti-inflammatory potency of Mesua ferrea, Saraca asoca, Viscum album & Anthocephalus cadamba in murine macrophages raw 264.7 cell lines and Wistar albino rats
The present study reports the in-vitro and in-vivo anti-inflammatory potency of Mesua ferrea, Saraca asoca, Viscum album and Anthocephalus cadamba, potent medicinal plants of Western Ghats of Karnataka India. Lipopolysaccharide (LPS)-activated murine macrophage RAW 264.7 cell line was used in in-vit...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2018-12-01
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| Series: | Beni-Suef University Journal of Basic and Applied Sciences |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2314853518302646 |
| _version_ | 1818111289016188928 |
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| author | Syed Murthuza B.K. Manjunatha |
| author_facet | Syed Murthuza B.K. Manjunatha |
| author_sort | Syed Murthuza |
| collection | DOAJ |
| description | The present study reports the in-vitro and in-vivo anti-inflammatory potency of Mesua ferrea, Saraca asoca, Viscum album and Anthocephalus cadamba, potent medicinal plants of Western Ghats of Karnataka India. Lipopolysaccharide (LPS)-activated murine macrophage RAW 264.7 cell line was used in in-vitro assay. Pretreatment with methanolic extracts at non toxic concentrations abridged the LPS-induced protein levels. Among the four plants tested, methanolic extract of Viscum album (70.20 ± 1.094) and Mesua ferrea (68.29 ± 2.862) showed potent anti-inflammatory activity at 100 µg concentration with an IC50 value of 57.23 ± 1.922 and 63.36 ± 3.791 µg/ml. In vivo anti-inflammatory activity was evaluated using carrageenan-induced paw edema in Wistar albino rats, Viscum album (0.6167 ± 0.01667) and Mesua ferrea (0.6833 ± 0.01667) showed potent anti-inflammatory activity (at 100 mg/kg.bw). The obtained results indicate the potency of active constituents of Mesua ferrea and Viscum album in the development of effective anti-inflammatory drugs. Keywords: Antiinflammatory, Lipopolysaccharide, Paw edema, Medicinal plants |
| first_indexed | 2024-12-11T03:00:40Z |
| format | Article |
| id | doaj.art-63175a8683e94e89bf7f426010851f1e |
| institution | Directory Open Access Journal |
| issn | 2314-8535 |
| language | English |
| last_indexed | 2024-12-11T03:00:40Z |
| publishDate | 2018-12-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Beni-Suef University Journal of Basic and Applied Sciences |
| spelling | doaj.art-63175a8683e94e89bf7f426010851f1e2022-12-22T01:23:03ZengSpringerOpenBeni-Suef University Journal of Basic and Applied Sciences2314-85352018-12-0174719723In vitro and in vivo evaluation of anti-inflammatory potency of Mesua ferrea, Saraca asoca, Viscum album & Anthocephalus cadamba in murine macrophages raw 264.7 cell lines and Wistar albino ratsSyed Murthuza0B.K. Manjunatha1Department of Biotechnology, The Oxford College of Engineering, Bommanahalli, Bengaluru 560068, IndiaCorresponding author.; Department of Biotechnology, The Oxford College of Engineering, Bommanahalli, Bengaluru 560068, IndiaThe present study reports the in-vitro and in-vivo anti-inflammatory potency of Mesua ferrea, Saraca asoca, Viscum album and Anthocephalus cadamba, potent medicinal plants of Western Ghats of Karnataka India. Lipopolysaccharide (LPS)-activated murine macrophage RAW 264.7 cell line was used in in-vitro assay. Pretreatment with methanolic extracts at non toxic concentrations abridged the LPS-induced protein levels. Among the four plants tested, methanolic extract of Viscum album (70.20 ± 1.094) and Mesua ferrea (68.29 ± 2.862) showed potent anti-inflammatory activity at 100 µg concentration with an IC50 value of 57.23 ± 1.922 and 63.36 ± 3.791 µg/ml. In vivo anti-inflammatory activity was evaluated using carrageenan-induced paw edema in Wistar albino rats, Viscum album (0.6167 ± 0.01667) and Mesua ferrea (0.6833 ± 0.01667) showed potent anti-inflammatory activity (at 100 mg/kg.bw). The obtained results indicate the potency of active constituents of Mesua ferrea and Viscum album in the development of effective anti-inflammatory drugs. Keywords: Antiinflammatory, Lipopolysaccharide, Paw edema, Medicinal plantshttp://www.sciencedirect.com/science/article/pii/S2314853518302646 |
| spellingShingle | Syed Murthuza B.K. Manjunatha In vitro and in vivo evaluation of anti-inflammatory potency of Mesua ferrea, Saraca asoca, Viscum album & Anthocephalus cadamba in murine macrophages raw 264.7 cell lines and Wistar albino rats Beni-Suef University Journal of Basic and Applied Sciences |
| title | In vitro and in vivo evaluation of anti-inflammatory potency of Mesua ferrea, Saraca asoca, Viscum album & Anthocephalus cadamba in murine macrophages raw 264.7 cell lines and Wistar albino rats |
| title_full | In vitro and in vivo evaluation of anti-inflammatory potency of Mesua ferrea, Saraca asoca, Viscum album & Anthocephalus cadamba in murine macrophages raw 264.7 cell lines and Wistar albino rats |
| title_fullStr | In vitro and in vivo evaluation of anti-inflammatory potency of Mesua ferrea, Saraca asoca, Viscum album & Anthocephalus cadamba in murine macrophages raw 264.7 cell lines and Wistar albino rats |
| title_full_unstemmed | In vitro and in vivo evaluation of anti-inflammatory potency of Mesua ferrea, Saraca asoca, Viscum album & Anthocephalus cadamba in murine macrophages raw 264.7 cell lines and Wistar albino rats |
| title_short | In vitro and in vivo evaluation of anti-inflammatory potency of Mesua ferrea, Saraca asoca, Viscum album & Anthocephalus cadamba in murine macrophages raw 264.7 cell lines and Wistar albino rats |
| title_sort | in vitro and in vivo evaluation of anti inflammatory potency of mesua ferrea saraca asoca viscum album anthocephalus cadamba in murine macrophages raw 264 7 cell lines and wistar albino rats |
| url | http://www.sciencedirect.com/science/article/pii/S2314853518302646 |
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