Antialcohol and Hepatoprotective Effects of Tamarind Shell Extract on Ethanol-Induced Damage to HepG2 Cells and Animal Models
Alcohol liver disease (ALD) is one of the leading outcomes of acute and chronic liver injury. Accumulative evidence has confirmed that oxidative stress is involved in the development of ALD. In this study, we used chick embryos to establish ALD model to study the hepatoprotective effects of tamarind...
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MDPI AG
2023-03-01
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| Online Access: | https://www.mdpi.com/2304-8158/12/5/1078 |
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| author | Shao-Cong Han Rong-Ping Huang Qiong-Yi Zhang Chang-Yu Yan Xi-You Li Yi-Fang Li Rong-Rong He Wei-Xi Li |
| author_facet | Shao-Cong Han Rong-Ping Huang Qiong-Yi Zhang Chang-Yu Yan Xi-You Li Yi-Fang Li Rong-Rong He Wei-Xi Li |
| author_sort | Shao-Cong Han |
| collection | DOAJ |
| description | Alcohol liver disease (ALD) is one of the leading outcomes of acute and chronic liver injury. Accumulative evidence has confirmed that oxidative stress is involved in the development of ALD. In this study, we used chick embryos to establish ALD model to study the hepatoprotective effects of tamarind shell exttract (TSE). Chick embryos received 25% ethanol (75 μL) and TSE (250, 500, 750 μg/egg/75 μL) from embryonic development day (EDD) 5.5. Both ethanol and TSE were administrated every two days until EDD15. Ethanol-exposed zebrafish and HepG2 cell model were also employed. The results suggested that TSE effectively reversed the pathological changes, liver dysfunction and ethanol-metabolic enzyme disorder in ethanol-treated chick embryo liver, zebrafish and HepG2 cells. TSE suppressed the excessive reactive oxygen species (ROS) in zebrafish and HepG2 cells, as well as rebuilt the irrupted mitochondrial membrane potential. Meanwhile, the declined antioxidative activity of glutathione peroxidase (GPx) and superoxide dismutase (SOD), together with the content of total glutathione (T-GSH) were recovered by TSE. Moreover, TSE upregulated nuclear factor erythroid 2—related factor 2 (NRF2) and heme oxyense-1 (HO-1) expression in protein and mRNA level. All the phenomena suggested that TSE attenuated ALD through activating NRF2 to repress the oxidative stress induced by ethanol. |
| first_indexed | 2024-03-11T07:24:28Z |
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| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T07:24:28Z |
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| series | Foods |
| spelling | doaj.art-6318a3913a024c51847212133f560f1c2023-11-17T07:41:53ZengMDPI AGFoods2304-81582023-03-01125107810.3390/foods12051078Antialcohol and Hepatoprotective Effects of Tamarind Shell Extract on Ethanol-Induced Damage to HepG2 Cells and Animal ModelsShao-Cong Han0Rong-Ping Huang1Qiong-Yi Zhang2Chang-Yu Yan3Xi-You Li4Yi-Fang Li5Rong-Rong He6Wei-Xi Li7College of Traditional Chinese Medicine, Yunnan University of Chinese Medicine, Kunming 650500, ChinaCollege of Traditional Chinese Medicine, Yunnan University of Chinese Medicine, Kunming 650500, ChinaGuangdong Engineering Research Centre of Chinese Medicine & Disease Susceptibility, Jinan University, Guangzhou 510632, ChinaGuangdong Engineering Research Centre of Chinese Medicine & Disease Susceptibility, Jinan University, Guangzhou 510632, ChinaCollege of Traditional Chinese Medicine, Yunnan University of Chinese Medicine, Kunming 650500, ChinaGuangdong Engineering Research Centre of Chinese Medicine & Disease Susceptibility, Jinan University, Guangzhou 510632, ChinaGuangdong Engineering Research Centre of Chinese Medicine & Disease Susceptibility, Jinan University, Guangzhou 510632, ChinaCollege of Traditional Chinese Medicine, Yunnan University of Chinese Medicine, Kunming 650500, ChinaAlcohol liver disease (ALD) is one of the leading outcomes of acute and chronic liver injury. Accumulative evidence has confirmed that oxidative stress is involved in the development of ALD. In this study, we used chick embryos to establish ALD model to study the hepatoprotective effects of tamarind shell exttract (TSE). Chick embryos received 25% ethanol (75 μL) and TSE (250, 500, 750 μg/egg/75 μL) from embryonic development day (EDD) 5.5. Both ethanol and TSE were administrated every two days until EDD15. Ethanol-exposed zebrafish and HepG2 cell model were also employed. The results suggested that TSE effectively reversed the pathological changes, liver dysfunction and ethanol-metabolic enzyme disorder in ethanol-treated chick embryo liver, zebrafish and HepG2 cells. TSE suppressed the excessive reactive oxygen species (ROS) in zebrafish and HepG2 cells, as well as rebuilt the irrupted mitochondrial membrane potential. Meanwhile, the declined antioxidative activity of glutathione peroxidase (GPx) and superoxide dismutase (SOD), together with the content of total glutathione (T-GSH) were recovered by TSE. Moreover, TSE upregulated nuclear factor erythroid 2—related factor 2 (NRF2) and heme oxyense-1 (HO-1) expression in protein and mRNA level. All the phenomena suggested that TSE attenuated ALD through activating NRF2 to repress the oxidative stress induced by ethanol.https://www.mdpi.com/2304-8158/12/5/1078alcohol liver diseasechick embryotamarind shell extractoxidative stressNRF2 |
| spellingShingle | Shao-Cong Han Rong-Ping Huang Qiong-Yi Zhang Chang-Yu Yan Xi-You Li Yi-Fang Li Rong-Rong He Wei-Xi Li Antialcohol and Hepatoprotective Effects of Tamarind Shell Extract on Ethanol-Induced Damage to HepG2 Cells and Animal Models Foods alcohol liver disease chick embryo tamarind shell extract oxidative stress NRF2 |
| title | Antialcohol and Hepatoprotective Effects of Tamarind Shell Extract on Ethanol-Induced Damage to HepG2 Cells and Animal Models |
| title_full | Antialcohol and Hepatoprotective Effects of Tamarind Shell Extract on Ethanol-Induced Damage to HepG2 Cells and Animal Models |
| title_fullStr | Antialcohol and Hepatoprotective Effects of Tamarind Shell Extract on Ethanol-Induced Damage to HepG2 Cells and Animal Models |
| title_full_unstemmed | Antialcohol and Hepatoprotective Effects of Tamarind Shell Extract on Ethanol-Induced Damage to HepG2 Cells and Animal Models |
| title_short | Antialcohol and Hepatoprotective Effects of Tamarind Shell Extract on Ethanol-Induced Damage to HepG2 Cells and Animal Models |
| title_sort | antialcohol and hepatoprotective effects of tamarind shell extract on ethanol induced damage to hepg2 cells and animal models |
| topic | alcohol liver disease chick embryo tamarind shell extract oxidative stress NRF2 |
| url | https://www.mdpi.com/2304-8158/12/5/1078 |
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