Synergistic utilization of carbon sources for efficient biosynthesis of N-acetylglucosamine
N-acetylglucosamine (GlcNAc) is an amino monosaccharide that has a variety of bioactivities and is widely used in pharmaceutical and food industries. Production of GlcNAc by chitin hydrolysis is limited by the supply of raw materials and encounters the risk of shellfish protein contamination. For ef...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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KeAi Communications Co. Ltd.
2023-06-01
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| Series: | Green Chemical Engineering |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666952822000292 |
| _version_ | 1797812029414703104 |
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| author | Yanni Pei Yuhan Wang Xiaolin Shen Jia Wang Xinxiao Sun Qipeng Yuan |
| author_facet | Yanni Pei Yuhan Wang Xiaolin Shen Jia Wang Xinxiao Sun Qipeng Yuan |
| author_sort | Yanni Pei |
| collection | DOAJ |
| description | N-acetylglucosamine (GlcNAc) is an amino monosaccharide that has a variety of bioactivities and is widely used in pharmaceutical and food industries. Production of GlcNAc by chitin hydrolysis is limited by the supply of raw materials and encounters the risk of shellfish protein contamination. For efficient biosynthesis of GlcNAc, one challenge is to balance the carbon distribution between growth and production. Here, we applied the strategy of synergistic carbon utilization, in which glycerol supports cell growth and provides the acetyl group of GlcNAc while glucose serves as the precursor to glucosamine. The efficiency of GlcNAc production was stepwise improved by blocking the product re-uptake and degradation, strengthening the biosynthetic pathway and synergistically utilizing two carbon sources. With these efforts, the final strain produced 41.5 g/L GlcNAc with a yield of 0.49 g/g of total carbon sources. In addition, we also explored the feasibility of using acetate as a cheap carbon source to partly replace glycerol. This study provides a promising alternative strategy for sustainable and efficient production of GlcNAc. |
| first_indexed | 2024-03-13T07:32:28Z |
| format | Article |
| id | doaj.art-6320a92a8c9c49c3b5a75f2533b6c8f6 |
| institution | Directory Open Access Journal |
| issn | 2666-9528 |
| language | English |
| last_indexed | 2024-03-13T07:32:28Z |
| publishDate | 2023-06-01 |
| publisher | KeAi Communications Co. Ltd. |
| record_format | Article |
| series | Green Chemical Engineering |
| spelling | doaj.art-6320a92a8c9c49c3b5a75f2533b6c8f62023-06-04T04:24:33ZengKeAi Communications Co. Ltd.Green Chemical Engineering2666-95282023-06-0142233238Synergistic utilization of carbon sources for efficient biosynthesis of N-acetylglucosamineYanni Pei0Yuhan Wang1Xiaolin Shen2Jia Wang3Xinxiao Sun4Qipeng Yuan5State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, ChinaState Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, ChinaState Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, ChinaState Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, ChinaCorresponding author.; State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, ChinaCorresponding author.; State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, ChinaN-acetylglucosamine (GlcNAc) is an amino monosaccharide that has a variety of bioactivities and is widely used in pharmaceutical and food industries. Production of GlcNAc by chitin hydrolysis is limited by the supply of raw materials and encounters the risk of shellfish protein contamination. For efficient biosynthesis of GlcNAc, one challenge is to balance the carbon distribution between growth and production. Here, we applied the strategy of synergistic carbon utilization, in which glycerol supports cell growth and provides the acetyl group of GlcNAc while glucose serves as the precursor to glucosamine. The efficiency of GlcNAc production was stepwise improved by blocking the product re-uptake and degradation, strengthening the biosynthetic pathway and synergistically utilizing two carbon sources. With these efforts, the final strain produced 41.5 g/L GlcNAc with a yield of 0.49 g/g of total carbon sources. In addition, we also explored the feasibility of using acetate as a cheap carbon source to partly replace glycerol. This study provides a promising alternative strategy for sustainable and efficient production of GlcNAc.http://www.sciencedirect.com/science/article/pii/S2666952822000292N-AcetylglucosamineSynergistic carbon utilizationGlucose facilitated diffusion systemAcetate |
| spellingShingle | Yanni Pei Yuhan Wang Xiaolin Shen Jia Wang Xinxiao Sun Qipeng Yuan Synergistic utilization of carbon sources for efficient biosynthesis of N-acetylglucosamine Green Chemical Engineering N-Acetylglucosamine Synergistic carbon utilization Glucose facilitated diffusion system Acetate |
| title | Synergistic utilization of carbon sources for efficient biosynthesis of N-acetylglucosamine |
| title_full | Synergistic utilization of carbon sources for efficient biosynthesis of N-acetylglucosamine |
| title_fullStr | Synergistic utilization of carbon sources for efficient biosynthesis of N-acetylglucosamine |
| title_full_unstemmed | Synergistic utilization of carbon sources for efficient biosynthesis of N-acetylglucosamine |
| title_short | Synergistic utilization of carbon sources for efficient biosynthesis of N-acetylglucosamine |
| title_sort | synergistic utilization of carbon sources for efficient biosynthesis of n acetylglucosamine |
| topic | N-Acetylglucosamine Synergistic carbon utilization Glucose facilitated diffusion system Acetate |
| url | http://www.sciencedirect.com/science/article/pii/S2666952822000292 |
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