Monocytes from Uninfected Neonates Born to <i>Trypanosoma cruzi</i>-Infected Mothers Display Upregulated Capacity to Produce TNF-α and to Control Infection in Association with Maternally Transferred Antibodies
Activated monocytes/macrophages that produce inflammatory cytokines and nitric oxide are crucial for controlling <i>Trypanosoma cruzi</i> infection. We previously showed that uninfected newborns from <i>T. cruzi</i> infected mothers (M+B- newborns) were sensitized to produce...
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MDPI AG
2023-08-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/12/9/1103 |
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| author | Amilcar Flores Cristina Alonso-Vega Emmanuel Hermann Mary-Cruz Torrico Nair Alaide Montaño Villarroel Faustino Torrico Yves Carlier Carine Truyens |
| author_facet | Amilcar Flores Cristina Alonso-Vega Emmanuel Hermann Mary-Cruz Torrico Nair Alaide Montaño Villarroel Faustino Torrico Yves Carlier Carine Truyens |
| author_sort | Amilcar Flores |
| collection | DOAJ |
| description | Activated monocytes/macrophages that produce inflammatory cytokines and nitric oxide are crucial for controlling <i>Trypanosoma cruzi</i> infection. We previously showed that uninfected newborns from <i>T. cruzi</i> infected mothers (M+B- newborns) were sensitized to produce higher levels of inflammatory cytokines than newborns from uninfected mothers (M-B- newborns), suggesting that their monocytes were more activated. Thus, we wondered whether these cells might help limit congenital infection. We investigated this possibility by studying the activation status of M+B- cord blood monocytes and their ability to control <i>T. cruzi</i> in vitro infection. We showed that M+B- monocytes have an upregulated capacity to produce the inflammatory cytokine TNF-α and a better ability to control <i>T. cruzi</i> infection than M-B- monocytes. Our study also showed that <i>T. cruzi</i>-specific Abs transferred from the mother play a dual role by favoring trypomastigote entry into M+B- monocytes and inhibiting intracellular amastigote multiplication. These results support the possibility that some M+B- fetuses may eliminate the parasite transmitted in utero from their mothers, thus being uninfected at birth. |
| first_indexed | 2024-03-10T22:18:21Z |
| format | Article |
| id | doaj.art-6323a42c59854072a16e2fcd17c25726 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T22:18:21Z |
| publishDate | 2023-08-01 |
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| series | Pathogens |
| spelling | doaj.art-6323a42c59854072a16e2fcd17c257262023-11-19T12:22:04ZengMDPI AGPathogens2076-08172023-08-01129110310.3390/pathogens12091103Monocytes from Uninfected Neonates Born to <i>Trypanosoma cruzi</i>-Infected Mothers Display Upregulated Capacity to Produce TNF-α and to Control Infection in Association with Maternally Transferred AntibodiesAmilcar Flores0Cristina Alonso-Vega1Emmanuel Hermann2Mary-Cruz Torrico3Nair Alaide Montaño Villarroel4Faustino Torrico5Yves Carlier6Carine Truyens7Facultad de Medicina, Universidad Mayor de San Simon (U.M.S.S.), Cochabamba 2500, BoliviaFacultad de Medicina, Universidad Mayor de San Simon (U.M.S.S.), Cochabamba 2500, BoliviaLaboratory of Parasitology, Faculty of Medicine, ULB Center for Research in Immunology (UCRI), Université Libre de Bruxelles (U.L.B.), 1070 Brussels, BelgiumFacultad de Medicina, Universidad Mayor de San Simon (U.M.S.S.), Cochabamba 2500, BoliviaFacultad de Medicina, Universidad Mayor de San Simon (U.M.S.S.), Cochabamba 2500, BoliviaFacultad de Medicina, Universidad Mayor de San Simon (U.M.S.S.), Cochabamba 2500, BoliviaLaboratory of Parasitology, Faculty of Medicine, ULB Center for Research in Immunology (UCRI), Université Libre de Bruxelles (U.L.B.), 1070 Brussels, BelgiumLaboratory of Parasitology, Faculty of Medicine, ULB Center for Research in Immunology (UCRI), Université Libre de Bruxelles (U.L.B.), 1070 Brussels, BelgiumActivated monocytes/macrophages that produce inflammatory cytokines and nitric oxide are crucial for controlling <i>Trypanosoma cruzi</i> infection. We previously showed that uninfected newborns from <i>T. cruzi</i> infected mothers (M+B- newborns) were sensitized to produce higher levels of inflammatory cytokines than newborns from uninfected mothers (M-B- newborns), suggesting that their monocytes were more activated. Thus, we wondered whether these cells might help limit congenital infection. We investigated this possibility by studying the activation status of M+B- cord blood monocytes and their ability to control <i>T. cruzi</i> in vitro infection. We showed that M+B- monocytes have an upregulated capacity to produce the inflammatory cytokine TNF-α and a better ability to control <i>T. cruzi</i> infection than M-B- monocytes. Our study also showed that <i>T. cruzi</i>-specific Abs transferred from the mother play a dual role by favoring trypomastigote entry into M+B- monocytes and inhibiting intracellular amastigote multiplication. These results support the possibility that some M+B- fetuses may eliminate the parasite transmitted in utero from their mothers, thus being uninfected at birth.https://www.mdpi.com/2076-0817/12/9/1103congenital chagas diseasemonocytes<i>Trypanosoma cruzi</i>-specific antibodies |
| spellingShingle | Amilcar Flores Cristina Alonso-Vega Emmanuel Hermann Mary-Cruz Torrico Nair Alaide Montaño Villarroel Faustino Torrico Yves Carlier Carine Truyens Monocytes from Uninfected Neonates Born to <i>Trypanosoma cruzi</i>-Infected Mothers Display Upregulated Capacity to Produce TNF-α and to Control Infection in Association with Maternally Transferred Antibodies Pathogens congenital chagas disease monocytes <i>Trypanosoma cruzi</i>-specific antibodies |
| title | Monocytes from Uninfected Neonates Born to <i>Trypanosoma cruzi</i>-Infected Mothers Display Upregulated Capacity to Produce TNF-α and to Control Infection in Association with Maternally Transferred Antibodies |
| title_full | Monocytes from Uninfected Neonates Born to <i>Trypanosoma cruzi</i>-Infected Mothers Display Upregulated Capacity to Produce TNF-α and to Control Infection in Association with Maternally Transferred Antibodies |
| title_fullStr | Monocytes from Uninfected Neonates Born to <i>Trypanosoma cruzi</i>-Infected Mothers Display Upregulated Capacity to Produce TNF-α and to Control Infection in Association with Maternally Transferred Antibodies |
| title_full_unstemmed | Monocytes from Uninfected Neonates Born to <i>Trypanosoma cruzi</i>-Infected Mothers Display Upregulated Capacity to Produce TNF-α and to Control Infection in Association with Maternally Transferred Antibodies |
| title_short | Monocytes from Uninfected Neonates Born to <i>Trypanosoma cruzi</i>-Infected Mothers Display Upregulated Capacity to Produce TNF-α and to Control Infection in Association with Maternally Transferred Antibodies |
| title_sort | monocytes from uninfected neonates born to i trypanosoma cruzi i infected mothers display upregulated capacity to produce tnf α and to control infection in association with maternally transferred antibodies |
| topic | congenital chagas disease monocytes <i>Trypanosoma cruzi</i>-specific antibodies |
| url | https://www.mdpi.com/2076-0817/12/9/1103 |
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