The sampling efficiencies of commercial nasopharyngeal swabs
Upper respiratory tract samples are the most commonly used samples for coronavirus disease 2019 (COVID-19) diagnosis. The samples collected from the nasopharynx are preferred for viral nucleic acids detection. Commercial nasopharyngeal swabs (NPSs) are the major factor that influences the sampling q...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-04-01
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| Series: | Biosafety and Health |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590053622000295 |
| _version_ | 1811302859385339904 |
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| author | Ying Wang Geng Wang Lulu Zhang Lili Ren Jianwei Wang |
| author_facet | Ying Wang Geng Wang Lulu Zhang Lili Ren Jianwei Wang |
| author_sort | Ying Wang |
| collection | DOAJ |
| description | Upper respiratory tract samples are the most commonly used samples for coronavirus disease 2019 (COVID-19) diagnosis. The samples collected from the nasopharynx are preferred for viral nucleic acids detection. Commercial nasopharyngeal swabs (NPSs) are the major factor that influences the sampling quality. We here evaluated the acceptability and efficiency of NPSs from five manufacturers by examining the concentration of glyceraldehyde-3-phosphate dehydrogenase gene (GAPDH) retrieved from the swabs using the RT-PCR method. Significant different concentrations of GAPDH were detected, ranged from 4.36 × 108 copies/mL to 6.98 × 1010 copies/mL among the five swabs (p < 0.05). The designation of the swab head, with or without tip expansion, had limited influence on the collection efficiency. The discrepancy among the NPSs emphasized the improvement of the swab head material. |
| first_indexed | 2024-04-13T07:36:54Z |
| format | Article |
| id | doaj.art-632680dc7550405794f583b7a1f8e76c |
| institution | Directory Open Access Journal |
| issn | 2590-0536 |
| language | English |
| last_indexed | 2024-04-13T07:36:54Z |
| publishDate | 2022-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biosafety and Health |
| spelling | doaj.art-632680dc7550405794f583b7a1f8e76c2022-12-22T02:56:07ZengElsevierBiosafety and Health2590-05362022-04-01426669The sampling efficiencies of commercial nasopharyngeal swabsYing Wang0Geng Wang1Lulu Zhang2Lili Ren3Jianwei Wang4NHC Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, IPB, CAMS-Fondation Mérieux, Institute of Pathogen Biology (IPB), Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College, Beijing 100730, ChinaNHC Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, IPB, CAMS-Fondation Mérieux, Institute of Pathogen Biology (IPB), Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College, Beijing 100730, ChinaNHC Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, IPB, CAMS-Fondation Mérieux, Institute of Pathogen Biology (IPB), Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College, Beijing 100730, ChinaNHC Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, IPB, CAMS-Fondation Mérieux, Institute of Pathogen Biology (IPB), Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College, Beijing 100730, China; Key Laboratory of Respiratory Disease Pathogenomics, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China; Corresponding author: Chinese Academy of Medical Sciences & Peking Union Medical College, No.9 Dong Dan San Tiao, Dongcheng District, Beijing 100730, China.NHC Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, IPB, CAMS-Fondation Mérieux, Institute of Pathogen Biology (IPB), Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College, Beijing 100730, China; Key Laboratory of Respiratory Disease Pathogenomics, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, ChinaUpper respiratory tract samples are the most commonly used samples for coronavirus disease 2019 (COVID-19) diagnosis. The samples collected from the nasopharynx are preferred for viral nucleic acids detection. Commercial nasopharyngeal swabs (NPSs) are the major factor that influences the sampling quality. We here evaluated the acceptability and efficiency of NPSs from five manufacturers by examining the concentration of glyceraldehyde-3-phosphate dehydrogenase gene (GAPDH) retrieved from the swabs using the RT-PCR method. Significant different concentrations of GAPDH were detected, ranged from 4.36 × 108 copies/mL to 6.98 × 1010 copies/mL among the five swabs (p < 0.05). The designation of the swab head, with or without tip expansion, had limited influence on the collection efficiency. The discrepancy among the NPSs emphasized the improvement of the swab head material.http://www.sciencedirect.com/science/article/pii/S2590053622000295Nasopharyngeal swabsNucleic acidsEfficiencyDesignation |
| spellingShingle | Ying Wang Geng Wang Lulu Zhang Lili Ren Jianwei Wang The sampling efficiencies of commercial nasopharyngeal swabs Biosafety and Health Nasopharyngeal swabs Nucleic acids Efficiency Designation |
| title | The sampling efficiencies of commercial nasopharyngeal swabs |
| title_full | The sampling efficiencies of commercial nasopharyngeal swabs |
| title_fullStr | The sampling efficiencies of commercial nasopharyngeal swabs |
| title_full_unstemmed | The sampling efficiencies of commercial nasopharyngeal swabs |
| title_short | The sampling efficiencies of commercial nasopharyngeal swabs |
| title_sort | sampling efficiencies of commercial nasopharyngeal swabs |
| topic | Nasopharyngeal swabs Nucleic acids Efficiency Designation |
| url | http://www.sciencedirect.com/science/article/pii/S2590053622000295 |
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