Motor Unit Fatigability following Chronic Carnosine Supplementation in Aged Rats
Studies suggest that carnosine (beta-alanyl-L-histidine) is effective in treating neuromuscular diseases associated with aging, but there is still a need to clarify its role in motor units (MUs) function during aging. In this study, 40 male Wistar rats aged 15 months were randomly assigned to a cont...
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MDPI AG
2022-01-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/3/514 |
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| author | Dawid Łochyński Maciej Pawlak Inge Everaert Tomasz Podgórski Magdalena Gartych Anna-Maria Borucka Jan Celichowski Wim Derave Dominik Kaczmarek |
| author_facet | Dawid Łochyński Maciej Pawlak Inge Everaert Tomasz Podgórski Magdalena Gartych Anna-Maria Borucka Jan Celichowski Wim Derave Dominik Kaczmarek |
| author_sort | Dawid Łochyński |
| collection | DOAJ |
| description | Studies suggest that carnosine (beta-alanyl-L-histidine) is effective in treating neuromuscular diseases associated with aging, but there is still a need to clarify its role in motor units (MUs) function during aging. In this study, 40 male Wistar rats aged 15 months were randomly assigned to a control or to two experimental groups in which 0.1% carnosine supplementation was performed for 10 or 34 weeks. After 34 weeks, we examined fast fatigable (FF), fast fatigue-resistant (FR) and slow (S) MUs’ force properties and fatigability, as well as antioxidant potential, advanced glycation end products, activity of enzymes, and histidyl dipeptides content in the medial gastrocnemius muscle. Short- and long-term carnosine supplementation maintained the force of FF MUs at a higher level during its rapid decline seen from the initial 10 to 70 s of the fatigue test. In FF, especially long-term, and in FR MUs, especially short-term, carnosine supplementation resulted in less rapid force decline during the initial 70 s of the second fatigue protocol. Carnosine supplementation did not change muscle antioxidant potential and mortality rate (~35% in all groups), nor muscle mass with aging. Moreover, instead of the expected increase, a decrease in histidyl dipeptides by ~30% in the red portion of medial gastrocnemius muscle after long-term supplementation was found. After chronic carnosine supplementation, the specific changes in fatigue resistance were observed in FF and FR units, but not in S MU types that were not accompanied by an improvement of antioxidant potential and activity of glycolytic or oxidative enzymes in aged rats. These observations indicate that carnosine supplementation during aging may generate different physiological adaptations which should be considered as an important factor when planning treatment strategies. |
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| language | English |
| last_indexed | 2024-03-09T23:22:25Z |
| publishDate | 2022-01-01 |
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| series | Nutrients |
| spelling | doaj.art-6329b58f10d5422297a17131094c19f22023-11-23T17:25:02ZengMDPI AGNutrients2072-66432022-01-0114351410.3390/nu14030514Motor Unit Fatigability following Chronic Carnosine Supplementation in Aged RatsDawid Łochyński0Maciej Pawlak1Inge Everaert2Tomasz Podgórski3Magdalena Gartych4Anna-Maria Borucka5Jan Celichowski6Wim Derave7Dominik Kaczmarek8Department of Neuromuscular Physiotherapy, Poznan University of Physical Education, 61-879 Poznan, PolandDepartment of Physiology and Biochemistry, Poznan University of Physical Education, 61-879 Poznan, PolandDepartment of Movement and Sports Sciences, Ghent University, 9000 Ghent, BelgiumDepartment of Physiology and Biochemistry, Poznan University of Physical Education, 61-879 Poznan, PolandDepartment of Neurobiology, Poznan University of Physical Education, 61-879 Poznan, PolandDepartment of Physiology and Biochemistry, Poznan University of Physical Education, 61-879 Poznan, PolandDepartment of Neurobiology, Poznan University of Physical Education, 61-879 Poznan, PolandDepartment of Movement and Sports Sciences, Ghent University, 9000 Ghent, BelgiumDepartment of Physiology and Biochemistry, Poznan University of Physical Education, 61-879 Poznan, PolandStudies suggest that carnosine (beta-alanyl-L-histidine) is effective in treating neuromuscular diseases associated with aging, but there is still a need to clarify its role in motor units (MUs) function during aging. In this study, 40 male Wistar rats aged 15 months were randomly assigned to a control or to two experimental groups in which 0.1% carnosine supplementation was performed for 10 or 34 weeks. After 34 weeks, we examined fast fatigable (FF), fast fatigue-resistant (FR) and slow (S) MUs’ force properties and fatigability, as well as antioxidant potential, advanced glycation end products, activity of enzymes, and histidyl dipeptides content in the medial gastrocnemius muscle. Short- and long-term carnosine supplementation maintained the force of FF MUs at a higher level during its rapid decline seen from the initial 10 to 70 s of the fatigue test. In FF, especially long-term, and in FR MUs, especially short-term, carnosine supplementation resulted in less rapid force decline during the initial 70 s of the second fatigue protocol. Carnosine supplementation did not change muscle antioxidant potential and mortality rate (~35% in all groups), nor muscle mass with aging. Moreover, instead of the expected increase, a decrease in histidyl dipeptides by ~30% in the red portion of medial gastrocnemius muscle after long-term supplementation was found. After chronic carnosine supplementation, the specific changes in fatigue resistance were observed in FF and FR units, but not in S MU types that were not accompanied by an improvement of antioxidant potential and activity of glycolytic or oxidative enzymes in aged rats. These observations indicate that carnosine supplementation during aging may generate different physiological adaptations which should be considered as an important factor when planning treatment strategies.https://www.mdpi.com/2072-6643/14/3/514carnosineagingmotor unitsfatiguerat |
| spellingShingle | Dawid Łochyński Maciej Pawlak Inge Everaert Tomasz Podgórski Magdalena Gartych Anna-Maria Borucka Jan Celichowski Wim Derave Dominik Kaczmarek Motor Unit Fatigability following Chronic Carnosine Supplementation in Aged Rats Nutrients carnosine aging motor units fatigue rat |
| title | Motor Unit Fatigability following Chronic Carnosine Supplementation in Aged Rats |
| title_full | Motor Unit Fatigability following Chronic Carnosine Supplementation in Aged Rats |
| title_fullStr | Motor Unit Fatigability following Chronic Carnosine Supplementation in Aged Rats |
| title_full_unstemmed | Motor Unit Fatigability following Chronic Carnosine Supplementation in Aged Rats |
| title_short | Motor Unit Fatigability following Chronic Carnosine Supplementation in Aged Rats |
| title_sort | motor unit fatigability following chronic carnosine supplementation in aged rats |
| topic | carnosine aging motor units fatigue rat |
| url | https://www.mdpi.com/2072-6643/14/3/514 |
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