Identification of alcohol stress tolerance genes of Synechocystis sp. PCC 6803 using adaptive laboratory evolution
Abstract Background Synechocystis sp. PCC 6803 is an attractive organism for the production of alcohols, such as isobutanol and ethanol. However, because stress against the produced alcohol is a major barrier for industrial applications, it is highly desirable to engineer organisms with strong alcoh...
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| Format: | Article |
| Language: | English |
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BMC
2017-12-01
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| Series: | Biotechnology for Biofuels |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13068-017-0996-5 |
| _version_ | 1818233279633948672 |
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| author | Takuya Matsusako Yoshihiro Toya Katsunori Yoshikawa Hiroshi Shimizu |
| author_facet | Takuya Matsusako Yoshihiro Toya Katsunori Yoshikawa Hiroshi Shimizu |
| author_sort | Takuya Matsusako |
| collection | DOAJ |
| description | Abstract Background Synechocystis sp. PCC 6803 is an attractive organism for the production of alcohols, such as isobutanol and ethanol. However, because stress against the produced alcohol is a major barrier for industrial applications, it is highly desirable to engineer organisms with strong alcohol tolerance. Results Isobutanol-tolerant strains of Synechocystis sp. PCC 6803 were obtained by long-term passage culture experiments using medium containing 2 g/L isobutanol. These evolved strains grew on medium containing 5 g/L isobutanol on which the parental strain could not grow. Mutation analysis of the evolved strains revealed that they acquired resistance ability due to combinatorial malfunctions of slr1044 (mcpA) and slr0369 (envD), or slr0322 (hik43) and envD. The tolerant strains demonstrated stress resistance against isobutanol as well as a wide variety of alcohols such as ethanol, n-butanol, and isopentanol. As a result of introducing an ethanol-producing pathway into the evolved strain, its productivity successfully increased to 142% of the control strain. Conclusions Novel mutations were identified that improved the stress tolerance ability of various alcohols in Synechocystis sp. PCC 6803. |
| first_indexed | 2024-12-12T11:19:40Z |
| format | Article |
| id | doaj.art-632dcb2149944fbbbccd871ea9ec7146 |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-12-12T11:19:40Z |
| publishDate | 2017-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-632dcb2149944fbbbccd871ea9ec71462022-12-22T00:26:03ZengBMCBiotechnology for Biofuels1754-68342017-12-011011910.1186/s13068-017-0996-5Identification of alcohol stress tolerance genes of Synechocystis sp. PCC 6803 using adaptive laboratory evolutionTakuya Matsusako0Yoshihiro Toya1Katsunori Yoshikawa2Hiroshi Shimizu3Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka UniversityDepartment of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka UniversityDepartment of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka UniversityDepartment of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka UniversityAbstract Background Synechocystis sp. PCC 6803 is an attractive organism for the production of alcohols, such as isobutanol and ethanol. However, because stress against the produced alcohol is a major barrier for industrial applications, it is highly desirable to engineer organisms with strong alcohol tolerance. Results Isobutanol-tolerant strains of Synechocystis sp. PCC 6803 were obtained by long-term passage culture experiments using medium containing 2 g/L isobutanol. These evolved strains grew on medium containing 5 g/L isobutanol on which the parental strain could not grow. Mutation analysis of the evolved strains revealed that they acquired resistance ability due to combinatorial malfunctions of slr1044 (mcpA) and slr0369 (envD), or slr0322 (hik43) and envD. The tolerant strains demonstrated stress resistance against isobutanol as well as a wide variety of alcohols such as ethanol, n-butanol, and isopentanol. As a result of introducing an ethanol-producing pathway into the evolved strain, its productivity successfully increased to 142% of the control strain. Conclusions Novel mutations were identified that improved the stress tolerance ability of various alcohols in Synechocystis sp. PCC 6803.http://link.springer.com/article/10.1186/s13068-017-0996-5Synechocystis sp. PCC 6803Alcohol stress toleranceAdaptive laboratory evolutionIsobutanol |
| spellingShingle | Takuya Matsusako Yoshihiro Toya Katsunori Yoshikawa Hiroshi Shimizu Identification of alcohol stress tolerance genes of Synechocystis sp. PCC 6803 using adaptive laboratory evolution Biotechnology for Biofuels Synechocystis sp. PCC 6803 Alcohol stress tolerance Adaptive laboratory evolution Isobutanol |
| title | Identification of alcohol stress tolerance genes of Synechocystis sp. PCC 6803 using adaptive laboratory evolution |
| title_full | Identification of alcohol stress tolerance genes of Synechocystis sp. PCC 6803 using adaptive laboratory evolution |
| title_fullStr | Identification of alcohol stress tolerance genes of Synechocystis sp. PCC 6803 using adaptive laboratory evolution |
| title_full_unstemmed | Identification of alcohol stress tolerance genes of Synechocystis sp. PCC 6803 using adaptive laboratory evolution |
| title_short | Identification of alcohol stress tolerance genes of Synechocystis sp. PCC 6803 using adaptive laboratory evolution |
| title_sort | identification of alcohol stress tolerance genes of synechocystis sp pcc 6803 using adaptive laboratory evolution |
| topic | Synechocystis sp. PCC 6803 Alcohol stress tolerance Adaptive laboratory evolution Isobutanol |
| url | http://link.springer.com/article/10.1186/s13068-017-0996-5 |
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