Epinephrine Affects Ribosomes, Cell Division, and Catabolic Processes in <i>Micrococcus luteus</i> Skin Strain C01: Revelation of the Conditionally Extensive Hormone Effect Using Orbitrap Mass Spectrometry and Proteomic Analysis
In the current study, extensive Orbitrap mass spectrometry analysis was conducted for skin strain <i>Micrococcus luteus</i> C01 planktonic cultures and biofilms after 24 h and 72 h of incubation either in the presence of epinephrine or without any implementations. The investigation revea...
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| Jezik: | English |
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MDPI AG
2023-08-01
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| Serija: | Microorganisms |
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| Online pristup: | https://www.mdpi.com/2076-2607/11/9/2181 |
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| author | Andrei V. Gannesen Rustam H. Ziganshin Maria A. Ovcharova Ekaterina D. Nevolina Alena I. Klimko Sergey V. Martyanov Vladimir K. Plakunov |
| author_facet | Andrei V. Gannesen Rustam H. Ziganshin Maria A. Ovcharova Ekaterina D. Nevolina Alena I. Klimko Sergey V. Martyanov Vladimir K. Plakunov |
| author_sort | Andrei V. Gannesen |
| collection | DOAJ |
| description | In the current study, extensive Orbitrap mass spectrometry analysis was conducted for skin strain <i>Micrococcus luteus</i> C01 planktonic cultures and biofilms after 24 h and 72 h of incubation either in the presence of epinephrine or without any implementations. The investigation revealed the complex and conditionally extensive effect of epinephrine at concentrations closer to normal blood plasma concentrations on both planktonic cultures and biofilms of skin strain <i>M. luteus</i> C01. The concentrations of hundreds of proteins changed during the shift from planktonic growth mode to biofilm and hundreds of proteins were downregulated or upregulated in the presence of epinephrine. Ribosomal, TCA, and cell division proteins appear to be the most altered in their amounts in the presence of the hormone. Potentially, the regulatory mechanism of this process is connected with c-di-GMP and histidine kinases, which were affected by epinephrine in different samples. The phenomenon of epinephrine-based biofilm regulation in <i>M. luteus</i> C01 has wide implications for microbial endocrinology and other research areas. |
| first_indexed | 2024-03-10T22:27:00Z |
| format | Article |
| id | doaj.art-6330fa82bc8d4152831c97d8c86037fe |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T22:27:00Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-6330fa82bc8d4152831c97d8c86037fe2023-11-19T12:01:43ZengMDPI AGMicroorganisms2076-26072023-08-01119218110.3390/microorganisms11092181Epinephrine Affects Ribosomes, Cell Division, and Catabolic Processes in <i>Micrococcus luteus</i> Skin Strain C01: Revelation of the Conditionally Extensive Hormone Effect Using Orbitrap Mass Spectrometry and Proteomic AnalysisAndrei V. Gannesen0Rustam H. Ziganshin1Maria A. Ovcharova2Ekaterina D. Nevolina3Alena I. Klimko4Sergey V. Martyanov5Vladimir K. Plakunov6Federal Research Center “Fundamentals of Biotechnology”, Russian Academy of Sciences, 119071 Moscow, RussiaInstitute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, RussiaFederal Research Center “Fundamentals of Biotechnology”, Russian Academy of Sciences, 119071 Moscow, RussiaFederal Research Center “Fundamentals of Biotechnology”, Russian Academy of Sciences, 119071 Moscow, RussiaFederal Research Center “Fundamentals of Biotechnology”, Russian Academy of Sciences, 119071 Moscow, RussiaFederal Research Center “Fundamentals of Biotechnology”, Russian Academy of Sciences, 119071 Moscow, RussiaFederal Research Center “Fundamentals of Biotechnology”, Russian Academy of Sciences, 119071 Moscow, RussiaIn the current study, extensive Orbitrap mass spectrometry analysis was conducted for skin strain <i>Micrococcus luteus</i> C01 planktonic cultures and biofilms after 24 h and 72 h of incubation either in the presence of epinephrine or without any implementations. The investigation revealed the complex and conditionally extensive effect of epinephrine at concentrations closer to normal blood plasma concentrations on both planktonic cultures and biofilms of skin strain <i>M. luteus</i> C01. The concentrations of hundreds of proteins changed during the shift from planktonic growth mode to biofilm and hundreds of proteins were downregulated or upregulated in the presence of epinephrine. Ribosomal, TCA, and cell division proteins appear to be the most altered in their amounts in the presence of the hormone. Potentially, the regulatory mechanism of this process is connected with c-di-GMP and histidine kinases, which were affected by epinephrine in different samples. The phenomenon of epinephrine-based biofilm regulation in <i>M. luteus</i> C01 has wide implications for microbial endocrinology and other research areas.https://www.mdpi.com/2076-2607/11/9/2181<i>Micrococcus luteus</i>skin microbiotamicrobial endocrinologyOrbitrap mass spectrometrybiofilmsplanktonic cultures |
| spellingShingle | Andrei V. Gannesen Rustam H. Ziganshin Maria A. Ovcharova Ekaterina D. Nevolina Alena I. Klimko Sergey V. Martyanov Vladimir K. Plakunov Epinephrine Affects Ribosomes, Cell Division, and Catabolic Processes in <i>Micrococcus luteus</i> Skin Strain C01: Revelation of the Conditionally Extensive Hormone Effect Using Orbitrap Mass Spectrometry and Proteomic Analysis Microorganisms <i>Micrococcus luteus</i> skin microbiota microbial endocrinology Orbitrap mass spectrometry biofilms planktonic cultures |
| title | Epinephrine Affects Ribosomes, Cell Division, and Catabolic Processes in <i>Micrococcus luteus</i> Skin Strain C01: Revelation of the Conditionally Extensive Hormone Effect Using Orbitrap Mass Spectrometry and Proteomic Analysis |
| title_full | Epinephrine Affects Ribosomes, Cell Division, and Catabolic Processes in <i>Micrococcus luteus</i> Skin Strain C01: Revelation of the Conditionally Extensive Hormone Effect Using Orbitrap Mass Spectrometry and Proteomic Analysis |
| title_fullStr | Epinephrine Affects Ribosomes, Cell Division, and Catabolic Processes in <i>Micrococcus luteus</i> Skin Strain C01: Revelation of the Conditionally Extensive Hormone Effect Using Orbitrap Mass Spectrometry and Proteomic Analysis |
| title_full_unstemmed | Epinephrine Affects Ribosomes, Cell Division, and Catabolic Processes in <i>Micrococcus luteus</i> Skin Strain C01: Revelation of the Conditionally Extensive Hormone Effect Using Orbitrap Mass Spectrometry and Proteomic Analysis |
| title_short | Epinephrine Affects Ribosomes, Cell Division, and Catabolic Processes in <i>Micrococcus luteus</i> Skin Strain C01: Revelation of the Conditionally Extensive Hormone Effect Using Orbitrap Mass Spectrometry and Proteomic Analysis |
| title_sort | epinephrine affects ribosomes cell division and catabolic processes in i micrococcus luteus i skin strain c01 revelation of the conditionally extensive hormone effect using orbitrap mass spectrometry and proteomic analysis |
| topic | <i>Micrococcus luteus</i> skin microbiota microbial endocrinology Orbitrap mass spectrometry biofilms planktonic cultures |
| url | https://www.mdpi.com/2076-2607/11/9/2181 |
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