HuR (ELAVL1) Stabilizes <i>SOX9</i> mRNA and Promotes Migration and Invasion in Breast Cancer Cells
RNA-binding proteins play diverse roles in cancer, influencing various facets of the disease, including proliferation, apoptosis, angiogenesis, senescence, invasion, epithelial–mesenchymal transition (EMT), and metastasis. HuR, a known RBP, is recognized for stabilizing mRNAs containing AU-rich elem...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/16/2/384 |
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| author | Jesús Morillo-Bernal Patricia Pizarro-García Gema Moreno-Bueno Amparo Cano María J. Mazón Pilar Eraso Francisco Portillo |
| author_facet | Jesús Morillo-Bernal Patricia Pizarro-García Gema Moreno-Bueno Amparo Cano María J. Mazón Pilar Eraso Francisco Portillo |
| author_sort | Jesús Morillo-Bernal |
| collection | DOAJ |
| description | RNA-binding proteins play diverse roles in cancer, influencing various facets of the disease, including proliferation, apoptosis, angiogenesis, senescence, invasion, epithelial–mesenchymal transition (EMT), and metastasis. HuR, a known RBP, is recognized for stabilizing mRNAs containing AU-rich elements (AREs), although its complete repertoire of mRNA targets remains undefined. Through a bioinformatics analysis of the gene expression profile of the Hs578T basal-like triple-negative breast cancer cell line with silenced HuR, we have identified SOX9 as a potential HuR-regulated target. SOX9 is a transcription factor involved in promoting EMT, metastasis, survival, and the maintenance of cancer stem cells (CSCs) in triple-negative breast cancer. Ribonucleoprotein immunoprecipitation assays confirm a direct interaction between HuR and <i>SOX9</i> mRNA. The half-life of <i>SOX9</i> mRNA and the levels of SOX9 protein decreased in cells lacking HuR. Cells silenced for HuR exhibit reduced migration and invasion compared to control cells, a phenotype similar to that described for SOX9-silenced cells. |
| first_indexed | 2024-03-08T11:02:09Z |
| format | Article |
| id | doaj.art-633a6c123572433aa6fdae71a5850a0d |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-08T11:02:09Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-633a6c123572433aa6fdae71a5850a0d2024-01-26T15:37:10ZengMDPI AGCancers2072-66942024-01-0116238410.3390/cancers16020384HuR (ELAVL1) Stabilizes <i>SOX9</i> mRNA and Promotes Migration and Invasion in Breast Cancer CellsJesús Morillo-Bernal0Patricia Pizarro-García1Gema Moreno-Bueno2Amparo Cano3María J. Mazón4Pilar Eraso5Francisco Portillo6Departamento de Bioquímica UAM, Instituto de Investigaciones Biomédicas Sols-Morreale, CSIC-UAM, 28029 Madrid, SpainDepartamento de Bioquímica UAM, Instituto de Investigaciones Biomédicas Sols-Morreale, CSIC-UAM, 28029 Madrid, SpainDepartamento de Bioquímica UAM, Instituto de Investigaciones Biomédicas Sols-Morreale, CSIC-UAM, 28029 Madrid, SpainDepartamento de Bioquímica UAM, Instituto de Investigaciones Biomédicas Sols-Morreale, CSIC-UAM, 28029 Madrid, SpainDepartamento de Bioquímica UAM, Instituto de Investigaciones Biomédicas Sols-Morreale, CSIC-UAM, 28029 Madrid, SpainDepartamento de Bioquímica UAM, Instituto de Investigaciones Biomédicas Sols-Morreale, CSIC-UAM, 28029 Madrid, SpainDepartamento de Bioquímica UAM, Instituto de Investigaciones Biomédicas Sols-Morreale, CSIC-UAM, 28029 Madrid, SpainRNA-binding proteins play diverse roles in cancer, influencing various facets of the disease, including proliferation, apoptosis, angiogenesis, senescence, invasion, epithelial–mesenchymal transition (EMT), and metastasis. HuR, a known RBP, is recognized for stabilizing mRNAs containing AU-rich elements (AREs), although its complete repertoire of mRNA targets remains undefined. Through a bioinformatics analysis of the gene expression profile of the Hs578T basal-like triple-negative breast cancer cell line with silenced HuR, we have identified SOX9 as a potential HuR-regulated target. SOX9 is a transcription factor involved in promoting EMT, metastasis, survival, and the maintenance of cancer stem cells (CSCs) in triple-negative breast cancer. Ribonucleoprotein immunoprecipitation assays confirm a direct interaction between HuR and <i>SOX9</i> mRNA. The half-life of <i>SOX9</i> mRNA and the levels of SOX9 protein decreased in cells lacking HuR. Cells silenced for HuR exhibit reduced migration and invasion compared to control cells, a phenotype similar to that described for SOX9-silenced cells.https://www.mdpi.com/2072-6694/16/2/384HuRSOX9mRNA stabilitymigrationinvasionbreast cancer cells |
| spellingShingle | Jesús Morillo-Bernal Patricia Pizarro-García Gema Moreno-Bueno Amparo Cano María J. Mazón Pilar Eraso Francisco Portillo HuR (ELAVL1) Stabilizes <i>SOX9</i> mRNA and Promotes Migration and Invasion in Breast Cancer Cells Cancers HuR SOX9 mRNA stability migration invasion breast cancer cells |
| title | HuR (ELAVL1) Stabilizes <i>SOX9</i> mRNA and Promotes Migration and Invasion in Breast Cancer Cells |
| title_full | HuR (ELAVL1) Stabilizes <i>SOX9</i> mRNA and Promotes Migration and Invasion in Breast Cancer Cells |
| title_fullStr | HuR (ELAVL1) Stabilizes <i>SOX9</i> mRNA and Promotes Migration and Invasion in Breast Cancer Cells |
| title_full_unstemmed | HuR (ELAVL1) Stabilizes <i>SOX9</i> mRNA and Promotes Migration and Invasion in Breast Cancer Cells |
| title_short | HuR (ELAVL1) Stabilizes <i>SOX9</i> mRNA and Promotes Migration and Invasion in Breast Cancer Cells |
| title_sort | hur elavl1 stabilizes i sox9 i mrna and promotes migration and invasion in breast cancer cells |
| topic | HuR SOX9 mRNA stability migration invasion breast cancer cells |
| url | https://www.mdpi.com/2072-6694/16/2/384 |
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