Visual/Photoelectrochemical Off-On Sensor Based on Cu/Mn Double-Doped CeO<sub>2</sub> and Branched Sheet Embedded Cu<sub>2</sub>O/CuO Nanocubes
An integrated dual-signal bioassay was devised to fulfil thrombin (TB) ultrasensitive detection by integrating visualization with the photoelectrochemical technique based on G-quadruplex/hemin. During the process, branched sheet embedded copper-based oxides prepared with illumination and alkaline co...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/13/2/227 |
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| author | Huihui Shi Yanfei Che Yumeng Rong Jiajun Wang Yanhu Wang Jinghua Yu Yan Zhang |
| author_facet | Huihui Shi Yanfei Che Yumeng Rong Jiajun Wang Yanhu Wang Jinghua Yu Yan Zhang |
| author_sort | Huihui Shi |
| collection | DOAJ |
| description | An integrated dual-signal bioassay was devised to fulfil thrombin (TB) ultrasensitive detection by integrating visualization with the photoelectrochemical technique based on G-quadruplex/hemin. During the process, branched sheet embedded copper-based oxides prepared with illumination and alkaline condition play a vital role in obtaining the desirable photocurrent. The switchover of photoelectrochemical signal was realized by the adjustable distance between electron acceptor G-quadruplex/hemin and interface materials due to dissociation of the Cu/Mn double-doped cerium dioxide (CuMn@CeO<sub>2</sub>)/DNA caused by the addition of TB. Then, CuMn@CeO<sub>2</sub> transferred onto visual zones triggered catalytic reactions under the existence of 3,3′,5,5′-tetramethylbenzidine and hydrogen peroxide, making a variation in color recognized by the naked eye and providing visual prediction. Under optimized conditions, this bioassay protocol demonstrated wide linear ranges (0.0001–50 nM), high selectivity, stability, and reproducibility. More importantly, the proposed visual/photoelectrochemical transduction mechanism platform exhibits a lower background signal and more reliable detection results, which also offers an effective way for detecting other proteins and nucleic acids. |
| first_indexed | 2024-03-11T09:04:59Z |
| format | Article |
| id | doaj.art-633a76f2648c4ad3bd330e3740b64d6c |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-11T09:04:59Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-633a76f2648c4ad3bd330e3740b64d6c2023-11-16T19:25:52ZengMDPI AGBiosensors2079-63742023-02-0113222710.3390/bios13020227Visual/Photoelectrochemical Off-On Sensor Based on Cu/Mn Double-Doped CeO<sub>2</sub> and Branched Sheet Embedded Cu<sub>2</sub>O/CuO NanocubesHuihui Shi0Yanfei Che1Yumeng Rong2Jiajun Wang3Yanhu Wang4Jinghua Yu5Yan Zhang6School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, ChinaSchool of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, ChinaSchool of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, ChinaSchool of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, ChinaShandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, ChinaSchool of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, ChinaSchool of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, ChinaAn integrated dual-signal bioassay was devised to fulfil thrombin (TB) ultrasensitive detection by integrating visualization with the photoelectrochemical technique based on G-quadruplex/hemin. During the process, branched sheet embedded copper-based oxides prepared with illumination and alkaline condition play a vital role in obtaining the desirable photocurrent. The switchover of photoelectrochemical signal was realized by the adjustable distance between electron acceptor G-quadruplex/hemin and interface materials due to dissociation of the Cu/Mn double-doped cerium dioxide (CuMn@CeO<sub>2</sub>)/DNA caused by the addition of TB. Then, CuMn@CeO<sub>2</sub> transferred onto visual zones triggered catalytic reactions under the existence of 3,3′,5,5′-tetramethylbenzidine and hydrogen peroxide, making a variation in color recognized by the naked eye and providing visual prediction. Under optimized conditions, this bioassay protocol demonstrated wide linear ranges (0.0001–50 nM), high selectivity, stability, and reproducibility. More importantly, the proposed visual/photoelectrochemical transduction mechanism platform exhibits a lower background signal and more reliable detection results, which also offers an effective way for detecting other proteins and nucleic acids.https://www.mdpi.com/2079-6374/13/2/227photoelectrochemicalcolorimetricthrombinpaper-based deviceCu<sub>2</sub>O/CuOCuMn@CeO<sub>2</sub> |
| spellingShingle | Huihui Shi Yanfei Che Yumeng Rong Jiajun Wang Yanhu Wang Jinghua Yu Yan Zhang Visual/Photoelectrochemical Off-On Sensor Based on Cu/Mn Double-Doped CeO<sub>2</sub> and Branched Sheet Embedded Cu<sub>2</sub>O/CuO Nanocubes Biosensors photoelectrochemical colorimetric thrombin paper-based device Cu<sub>2</sub>O/CuO CuMn@CeO<sub>2</sub> |
| title | Visual/Photoelectrochemical Off-On Sensor Based on Cu/Mn Double-Doped CeO<sub>2</sub> and Branched Sheet Embedded Cu<sub>2</sub>O/CuO Nanocubes |
| title_full | Visual/Photoelectrochemical Off-On Sensor Based on Cu/Mn Double-Doped CeO<sub>2</sub> and Branched Sheet Embedded Cu<sub>2</sub>O/CuO Nanocubes |
| title_fullStr | Visual/Photoelectrochemical Off-On Sensor Based on Cu/Mn Double-Doped CeO<sub>2</sub> and Branched Sheet Embedded Cu<sub>2</sub>O/CuO Nanocubes |
| title_full_unstemmed | Visual/Photoelectrochemical Off-On Sensor Based on Cu/Mn Double-Doped CeO<sub>2</sub> and Branched Sheet Embedded Cu<sub>2</sub>O/CuO Nanocubes |
| title_short | Visual/Photoelectrochemical Off-On Sensor Based on Cu/Mn Double-Doped CeO<sub>2</sub> and Branched Sheet Embedded Cu<sub>2</sub>O/CuO Nanocubes |
| title_sort | visual photoelectrochemical off on sensor based on cu mn double doped ceo sub 2 sub and branched sheet embedded cu sub 2 sub o cuo nanocubes |
| topic | photoelectrochemical colorimetric thrombin paper-based device Cu<sub>2</sub>O/CuO CuMn@CeO<sub>2</sub> |
| url | https://www.mdpi.com/2079-6374/13/2/227 |
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