Bacteria Isolated from Ricinus communis and Its Possible Application in Agroecology
Ricinus communis is a plant that is characterized by its different applications, in addition to being a wild plant favors its inclusion in agroecological practices because it does not represent high costs for producers. The aim of the work was to evaluate the bacterial isolates of R. communis with...
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| Format: | Article |
| Language: | English |
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Journal of Pure and Applied Microbiology
2019-06-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/bacteria-isolated-from-ricinus-communis-and-its-possible-application-in-agroecology/ |
| _version_ | 1819293409369653248 |
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| author | Marisol Hernandez-Ramirez Antonio Rivera Fernando Hernandez-Aldana Edith Chavez-Bravo Omar Romero-Arenas |
| author_facet | Marisol Hernandez-Ramirez Antonio Rivera Fernando Hernandez-Aldana Edith Chavez-Bravo Omar Romero-Arenas |
| author_sort | Marisol Hernandez-Ramirez |
| collection | DOAJ |
| description | Ricinus communis is a plant that is characterized by its different applications, in addition to being a
wild plant favors its inclusion in agroecological practices because it does not represent high costs for
producers. The aim of the work was to evaluate the bacterial isolates of R. communis with respect
to its inhibitory activity, chitinolytic, DNase, solubilization of phosphate, biofilm formation and
growth promoting effect in Allium sativum and Medicago sativa. The collection of R. communis
leaves was carried, which were washed and disinfected with sodium hypochlorite solution in order
to isolate bacteria in nutritive agar. The isolates obtained were identified by PCR amplification with
the oligonucleotides RM and RB and the amplified products were sequenced for identification. The
following tests were carried out on each of the isolates: growth inhibition vs Fusarium oxysporum,
chitinolytic activity, DNase, solubilization of phosphate, biofilm formation and growth evaluation
in A. sativum and M. sativa. Ten isolates were obtained that were identified as Pseudomonas 50%,
Enterobacter 30% and Bacillus 20%. Growth inhibition evaluations against F. oxysporum showed that
Bacillus showed significant inhibitory activity. The total isolates were negative for chitinolytic activity,
50% were positive for DNase, 40% positive for solubilization of phosphate and for biofilm formation
25% promoted weak formation and 10% moderate. Both plant models showed an increase in the
biomass of fresh weight and dry weight of their roots when they were inoculated with the bacterial
consortium at 50%, 75% and 100%. |
| first_indexed | 2024-12-24T04:09:58Z |
| format | Article |
| id | doaj.art-633c13c085b04605af78bcc96da47515 |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-12-24T04:09:58Z |
| publishDate | 2019-06-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-633c13c085b04605af78bcc96da475152022-12-21T17:16:05ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2019-06-0113268969510.22207/JPAM.13.2.05Bacteria Isolated from Ricinus communis and Its Possible Application in AgroecologyMarisol Hernandez-Ramirez0Antonio Rivera1Fernando Hernandez-Aldana2Edith Chavez-Bravo3Omar Romero-Arenas4Posgraduate in Sustainable Management of Agroecosystems, Science Institute of Benemerita Universidad Autonoma de Puebla. Building VAL 1, Km 1.7 Carretera a San Baltazar Tetela, San Pedro Zacachimalpa C.P. 72960, Puebla-Mexico.Research Center of Microbiology Sciences,, Science Institute of Benemerita Universidad Autonoma de Puebla. Building IC11-Ciudad Universitaria, Colonia San Manuel C.P. 72570, Puebla-Mexico.Chemistry Center, Science Institute of Benemerita Universidad Autonoma de Puebla. Ciudad Universitaria, Building IC8-Colonia San Manuel C.P. 72570, Puebla-Mexico.Research Center of Microbiology Sciences,, Science Institute of Benemerita Universidad Autonoma de Puebla. Building IC11-Ciudad Universitaria, Colonia San Manuel C.P. 72570, Puebla-Mexico.Agroecology Center, Science Institute of Benemerita Universidad Autonoma de Puebla. Building VAL 1, Km 1.7 Carretera a San Baltazar Tetela, San Pedro Zacachimalpa C.P. 72960, Puebla, Mexico.Ricinus communis is a plant that is characterized by its different applications, in addition to being a wild plant favors its inclusion in agroecological practices because it does not represent high costs for producers. The aim of the work was to evaluate the bacterial isolates of R. communis with respect to its inhibitory activity, chitinolytic, DNase, solubilization of phosphate, biofilm formation and growth promoting effect in Allium sativum and Medicago sativa. The collection of R. communis leaves was carried, which were washed and disinfected with sodium hypochlorite solution in order to isolate bacteria in nutritive agar. The isolates obtained were identified by PCR amplification with the oligonucleotides RM and RB and the amplified products were sequenced for identification. The following tests were carried out on each of the isolates: growth inhibition vs Fusarium oxysporum, chitinolytic activity, DNase, solubilization of phosphate, biofilm formation and growth evaluation in A. sativum and M. sativa. Ten isolates were obtained that were identified as Pseudomonas 50%, Enterobacter 30% and Bacillus 20%. Growth inhibition evaluations against F. oxysporum showed that Bacillus showed significant inhibitory activity. The total isolates were negative for chitinolytic activity, 50% were positive for DNase, 40% positive for solubilization of phosphate and for biofilm formation 25% promoted weak formation and 10% moderate. Both plant models showed an increase in the biomass of fresh weight and dry weight of their roots when they were inoculated with the bacterial consortium at 50%, 75% and 100%.https://microbiologyjournal.org/bacteria-isolated-from-ricinus-communis-and-its-possible-application-in-agroecology/castor wastepseudomonasenterobacterbacillusmetabolic activityinhibitionfusarium oxysporum |
| spellingShingle | Marisol Hernandez-Ramirez Antonio Rivera Fernando Hernandez-Aldana Edith Chavez-Bravo Omar Romero-Arenas Bacteria Isolated from Ricinus communis and Its Possible Application in Agroecology Journal of Pure and Applied Microbiology castor waste pseudomonas enterobacter bacillus metabolic activity inhibition fusarium oxysporum |
| title | Bacteria Isolated from Ricinus communis and Its Possible Application in Agroecology |
| title_full | Bacteria Isolated from Ricinus communis and Its Possible Application in Agroecology |
| title_fullStr | Bacteria Isolated from Ricinus communis and Its Possible Application in Agroecology |
| title_full_unstemmed | Bacteria Isolated from Ricinus communis and Its Possible Application in Agroecology |
| title_short | Bacteria Isolated from Ricinus communis and Its Possible Application in Agroecology |
| title_sort | bacteria isolated from ricinus communis and its possible application in agroecology |
| topic | castor waste pseudomonas enterobacter bacillus metabolic activity inhibition fusarium oxysporum |
| url | https://microbiologyjournal.org/bacteria-isolated-from-ricinus-communis-and-its-possible-application-in-agroecology/ |
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