Different responses of the rhizosphere microbiome to Verticillium dahliae infection in two cotton cultivars
Verticillium wilt is a disastrous disease caused by Verticillium dahliae that severely damages the production of cotton in China. Even under homogeneous conditions, the same cotton cultivar facing V. dahliae tends to either stay healthy or become seriously ill and die. This binary outcome may be rel...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1229454/full |
| _version_ | 1797744833658355712 |
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| author | Zhanjiang Tie Peng Wang Weijian Chen Binghui Tang Yu Yu Zheng Liu Sifeng Zhao Faisal Hayat Khan XueKun Zhang Hui Xi |
| author_facet | Zhanjiang Tie Peng Wang Weijian Chen Binghui Tang Yu Yu Zheng Liu Sifeng Zhao Faisal Hayat Khan XueKun Zhang Hui Xi |
| author_sort | Zhanjiang Tie |
| collection | DOAJ |
| description | Verticillium wilt is a disastrous disease caused by Verticillium dahliae that severely damages the production of cotton in China. Even under homogeneous conditions, the same cotton cultivar facing V. dahliae tends to either stay healthy or become seriously ill and die. This binary outcome may be related to the interactions between microbiome assembly and plant health. Understanding how the rhizosphere microbiome responds to V. dahliae infection is vital to controlling Verticillium wilt through the manipulation of the microbiome. In this study, we evaluated the healthy and diseased rhizosphere microbiome of two upland cotton cultivars that are resistant to V. dahliae, Zhong 2 (resistant) and Xin 36 (susceptible), using 16S rRNA and ITS high-throughput sequencing. The results showed that the healthy rhizosphere of both resistant cultivar and susceptible cultivar had more unique bacterial ASVs than the diseased rhizosphere, whereas fewer unique fungal ASVs were found in the healthy rhizosphere of resistant cultivar. There were no significant differences in alpha diversity and beta diversity between the resistant cultivar and susceptible cultivar. In both resistant cultivar and susceptible cultivar, bacterial genera such as Pseudomonas and Acidobacteria bacterium LP6, and fungal genera such as Cephalotrichum and Mortierella were both highly enriched in the diseased rhizosphere, and Pseudomonas abundance in diseased rhizospheres was significantly higher than that in the healthy rhizosphere regardless of the cultivar type. However, cultivar and V. dahliae infection can cause composition changes in the rhizosphere bacterial and fungal communities, especially in the relative abundances of core microbiome members, which varied significantly, with different responses in the two cotton cultivars. Analysis of co-occurrence networks showed that resistant cultivar has a more complex network relationship than susceptible cultivar in the bacterial communities, and V. dahliae has a significant impact on the bacterial community structure. These findings will further broaden the understanding of plant-rhizosphere microbiome interactions and provide an integrative perspective on the cotton rhizosphere microbiome, which is beneficial to cotton health and production. |
| first_indexed | 2024-03-12T15:14:57Z |
| format | Article |
| id | doaj.art-633df89cf16c4119af86d6efbf962b43 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-12T15:14:57Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-633df89cf16c4119af86d6efbf962b432023-08-11T14:48:48ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-08-011410.3389/fmicb.2023.12294541229454Different responses of the rhizosphere microbiome to Verticillium dahliae infection in two cotton cultivarsZhanjiang Tie0Peng Wang1Weijian Chen2Binghui Tang3Yu Yu4Zheng Liu5Sifeng Zhao6Faisal Hayat Khan7XueKun Zhang8Hui Xi9College of Agriculture, Shihezi University, Shihezi, Xinjiang, ChinaXinjiang Academy of Agricultural Reclamation Sciences, Shihezi, Xinjiang, ChinaCollege of Agriculture, Shihezi University, Shihezi, Xinjiang, ChinaCotton Research Institute, Shihezi Academy of Agricultural Sciences, Shihezi, Xinjiang, ChinaXinjiang Academy of Agricultural Reclamation Sciences, Shihezi, Xinjiang, ChinaCollege of Agriculture, Shihezi University, Shihezi, Xinjiang, ChinaCollege of Agriculture, Shihezi University, Shihezi, Xinjiang, ChinaCollege of Agriculture, Shihezi University, Shihezi, Xinjiang, ChinaCollege of Agriculture, Shihezi University, Shihezi, Xinjiang, ChinaCollege of Agriculture, Shihezi University, Shihezi, Xinjiang, ChinaVerticillium wilt is a disastrous disease caused by Verticillium dahliae that severely damages the production of cotton in China. Even under homogeneous conditions, the same cotton cultivar facing V. dahliae tends to either stay healthy or become seriously ill and die. This binary outcome may be related to the interactions between microbiome assembly and plant health. Understanding how the rhizosphere microbiome responds to V. dahliae infection is vital to controlling Verticillium wilt through the manipulation of the microbiome. In this study, we evaluated the healthy and diseased rhizosphere microbiome of two upland cotton cultivars that are resistant to V. dahliae, Zhong 2 (resistant) and Xin 36 (susceptible), using 16S rRNA and ITS high-throughput sequencing. The results showed that the healthy rhizosphere of both resistant cultivar and susceptible cultivar had more unique bacterial ASVs than the diseased rhizosphere, whereas fewer unique fungal ASVs were found in the healthy rhizosphere of resistant cultivar. There were no significant differences in alpha diversity and beta diversity between the resistant cultivar and susceptible cultivar. In both resistant cultivar and susceptible cultivar, bacterial genera such as Pseudomonas and Acidobacteria bacterium LP6, and fungal genera such as Cephalotrichum and Mortierella were both highly enriched in the diseased rhizosphere, and Pseudomonas abundance in diseased rhizospheres was significantly higher than that in the healthy rhizosphere regardless of the cultivar type. However, cultivar and V. dahliae infection can cause composition changes in the rhizosphere bacterial and fungal communities, especially in the relative abundances of core microbiome members, which varied significantly, with different responses in the two cotton cultivars. Analysis of co-occurrence networks showed that resistant cultivar has a more complex network relationship than susceptible cultivar in the bacterial communities, and V. dahliae has a significant impact on the bacterial community structure. These findings will further broaden the understanding of plant-rhizosphere microbiome interactions and provide an integrative perspective on the cotton rhizosphere microbiome, which is beneficial to cotton health and production.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1229454/fullcottonVerticillium dahliaerhizosphere microbiome16S rRNAITS |
| spellingShingle | Zhanjiang Tie Peng Wang Weijian Chen Binghui Tang Yu Yu Zheng Liu Sifeng Zhao Faisal Hayat Khan XueKun Zhang Hui Xi Different responses of the rhizosphere microbiome to Verticillium dahliae infection in two cotton cultivars Frontiers in Microbiology cotton Verticillium dahliae rhizosphere microbiome 16S rRNA ITS |
| title | Different responses of the rhizosphere microbiome to Verticillium dahliae infection in two cotton cultivars |
| title_full | Different responses of the rhizosphere microbiome to Verticillium dahliae infection in two cotton cultivars |
| title_fullStr | Different responses of the rhizosphere microbiome to Verticillium dahliae infection in two cotton cultivars |
| title_full_unstemmed | Different responses of the rhizosphere microbiome to Verticillium dahliae infection in two cotton cultivars |
| title_short | Different responses of the rhizosphere microbiome to Verticillium dahliae infection in two cotton cultivars |
| title_sort | different responses of the rhizosphere microbiome to verticillium dahliae infection in two cotton cultivars |
| topic | cotton Verticillium dahliae rhizosphere microbiome 16S rRNA ITS |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1229454/full |
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