Seasonal dynamics of microbial diversity in the rhizosphere of Ulmus pumila L. var. sabulosa in a steppe desert area of Northern China
The seasonal dynamics of microbial diversity within the rhizosphere of Ulmus pumila L. var. sabulosa in the hinterland of the Otindag Sandy Land of China were investigated using high-throughput sequencing of bacterial 16S rRNA genes and fungal ITS region sequences. A significant level of bacterial a...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PeerJ Inc.
2019-08-01
|
| Series: | PeerJ |
| Subjects: | |
| Online Access: | https://peerj.com/articles/7526.pdf |
| _version_ | 1827606013954490368 |
|---|---|
| author | Tianyu Liang Guang Yang Yunxia Ma Qingzhi Yao Yuan Ma Hui Ma Yang Hu Ying Yang Shaoxiong Wang Yiyong Pan Gangtie Li |
| author_facet | Tianyu Liang Guang Yang Yunxia Ma Qingzhi Yao Yuan Ma Hui Ma Yang Hu Ying Yang Shaoxiong Wang Yiyong Pan Gangtie Li |
| author_sort | Tianyu Liang |
| collection | DOAJ |
| description | The seasonal dynamics of microbial diversity within the rhizosphere of Ulmus pumila L. var. sabulosa in the hinterland of the Otindag Sandy Land of China were investigated using high-throughput sequencing of bacterial 16S rRNA genes and fungal ITS region sequences. A significant level of bacterial and fungal diversity was observed overall, with detection of 7,676 bacterial Operational Taxonomic Units (OTUs) belonging to 40 bacteria phyla and 3,582 fungal OTUs belonging to six phyla. Proteobacteria, Actinobacteria, and Firmicutes were the dominant bacterial phyla among communities, while Ascomycota, Basidiomycota, and Zygomycota were the dominant phyla of fungal communities. Seasonal changes influenced the α-diversity and β-diversity of bacterial communities within elm rhizospheres more than for fungal communities. Inferred functional analysis of the bacterial communities identified evidence for 41 level two KEGG (Kyoto Encyclopedia of Genes and Genomes) orthology groups, while guild-based analysis of the fungal communities identified eight ecological guilds. Metabolism was the most prevalent bacterial functional group, while saprotrophs prevailed among the identified fungal ecological guilds. Soil moisture and soil nutrient content were important factors that affected the microbial community structures of elm rhizospheres across seasons. The present pilot study provides an important baseline investigation of elm rhizosphere microbial communities. |
| first_indexed | 2024-03-09T06:31:00Z |
| format | Article |
| id | doaj.art-633e2b1bbf214964be4f703836bf19b4 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:31:00Z |
| publishDate | 2019-08-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-633e2b1bbf214964be4f703836bf19b42023-12-03T11:04:59ZengPeerJ Inc.PeerJ2167-83592019-08-017e752610.7717/peerj.7526Seasonal dynamics of microbial diversity in the rhizosphere of Ulmus pumila L. var. sabulosa in a steppe desert area of Northern ChinaTianyu Liang0Guang Yang1Yunxia Ma2Qingzhi Yao3Yuan Ma4Hui Ma5Yang Hu6Ying Yang7Shaoxiong Wang8Yiyong Pan9Gangtie Li10College of Desert Control Science and Engineering, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, ChinaCollege of Desert Control Science and Engineering, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, ChinaCollege of Desert Control Science and Engineering, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, ChinaCollege of Life Sciences, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, ChinaDesert Forest Experimental Center, China Academy of Forestry, Bayan Nur City, Inner Mongolia, ChinaCollege of Desert Control Science and Engineering, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, ChinaCollege of Desert Control Science and Engineering, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, ChinaCollege of Desert Control Science and Engineering, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, ChinaCollege of Desert Control Science and Engineering, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, ChinaCollege of Desert Control Science and Engineering, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, ChinaCollege of Desert Control Science and Engineering, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, ChinaThe seasonal dynamics of microbial diversity within the rhizosphere of Ulmus pumila L. var. sabulosa in the hinterland of the Otindag Sandy Land of China were investigated using high-throughput sequencing of bacterial 16S rRNA genes and fungal ITS region sequences. A significant level of bacterial and fungal diversity was observed overall, with detection of 7,676 bacterial Operational Taxonomic Units (OTUs) belonging to 40 bacteria phyla and 3,582 fungal OTUs belonging to six phyla. Proteobacteria, Actinobacteria, and Firmicutes were the dominant bacterial phyla among communities, while Ascomycota, Basidiomycota, and Zygomycota were the dominant phyla of fungal communities. Seasonal changes influenced the α-diversity and β-diversity of bacterial communities within elm rhizospheres more than for fungal communities. Inferred functional analysis of the bacterial communities identified evidence for 41 level two KEGG (Kyoto Encyclopedia of Genes and Genomes) orthology groups, while guild-based analysis of the fungal communities identified eight ecological guilds. Metabolism was the most prevalent bacterial functional group, while saprotrophs prevailed among the identified fungal ecological guilds. Soil moisture and soil nutrient content were important factors that affected the microbial community structures of elm rhizospheres across seasons. The present pilot study provides an important baseline investigation of elm rhizosphere microbial communities.https://peerj.com/articles/7526.pdfMicrobial communityUlmus pumila L. var. sabulosaThe Otindag Sandy LandSeasonal dynamicsRhizosphereSoil physicochemical properties |
| spellingShingle | Tianyu Liang Guang Yang Yunxia Ma Qingzhi Yao Yuan Ma Hui Ma Yang Hu Ying Yang Shaoxiong Wang Yiyong Pan Gangtie Li Seasonal dynamics of microbial diversity in the rhizosphere of Ulmus pumila L. var. sabulosa in a steppe desert area of Northern China PeerJ Microbial community Ulmus pumila L. var. sabulosa The Otindag Sandy Land Seasonal dynamics Rhizosphere Soil physicochemical properties |
| title | Seasonal dynamics of microbial diversity in the rhizosphere of Ulmus pumila L. var. sabulosa in a steppe desert area of Northern China |
| title_full | Seasonal dynamics of microbial diversity in the rhizosphere of Ulmus pumila L. var. sabulosa in a steppe desert area of Northern China |
| title_fullStr | Seasonal dynamics of microbial diversity in the rhizosphere of Ulmus pumila L. var. sabulosa in a steppe desert area of Northern China |
| title_full_unstemmed | Seasonal dynamics of microbial diversity in the rhizosphere of Ulmus pumila L. var. sabulosa in a steppe desert area of Northern China |
| title_short | Seasonal dynamics of microbial diversity in the rhizosphere of Ulmus pumila L. var. sabulosa in a steppe desert area of Northern China |
| title_sort | seasonal dynamics of microbial diversity in the rhizosphere of ulmus pumila l var sabulosa in a steppe desert area of northern china |
| topic | Microbial community Ulmus pumila L. var. sabulosa The Otindag Sandy Land Seasonal dynamics Rhizosphere Soil physicochemical properties |
| url | https://peerj.com/articles/7526.pdf |
| work_keys_str_mv | AT tianyuliang seasonaldynamicsofmicrobialdiversityintherhizosphereofulmuspumilalvarsabulosainasteppedesertareaofnorthernchina AT guangyang seasonaldynamicsofmicrobialdiversityintherhizosphereofulmuspumilalvarsabulosainasteppedesertareaofnorthernchina AT yunxiama seasonaldynamicsofmicrobialdiversityintherhizosphereofulmuspumilalvarsabulosainasteppedesertareaofnorthernchina AT qingzhiyao seasonaldynamicsofmicrobialdiversityintherhizosphereofulmuspumilalvarsabulosainasteppedesertareaofnorthernchina AT yuanma seasonaldynamicsofmicrobialdiversityintherhizosphereofulmuspumilalvarsabulosainasteppedesertareaofnorthernchina AT huima seasonaldynamicsofmicrobialdiversityintherhizosphereofulmuspumilalvarsabulosainasteppedesertareaofnorthernchina AT yanghu seasonaldynamicsofmicrobialdiversityintherhizosphereofulmuspumilalvarsabulosainasteppedesertareaofnorthernchina AT yingyang seasonaldynamicsofmicrobialdiversityintherhizosphereofulmuspumilalvarsabulosainasteppedesertareaofnorthernchina AT shaoxiongwang seasonaldynamicsofmicrobialdiversityintherhizosphereofulmuspumilalvarsabulosainasteppedesertareaofnorthernchina AT yiyongpan seasonaldynamicsofmicrobialdiversityintherhizosphereofulmuspumilalvarsabulosainasteppedesertareaofnorthernchina AT gangtieli seasonaldynamicsofmicrobialdiversityintherhizosphereofulmuspumilalvarsabulosainasteppedesertareaofnorthernchina |