Multi-locus analysis of human infective <it>Cryptosporidium </it>species and subtypes using ten novel genetic loci
<p>Abstract</p> <p>Background</p> <p><it>Cryptosporidium </it>is a protozoan parasite that causes diarrheal illness in a wide range of hosts including humans. Two species, <it>C. parvum </it>and <it>C. hominis </it>are of primary publ...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2010-08-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/10/213 |
| _version_ | 1818392565761703936 |
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| author | Bouzid Maha Tyler Kevin M Christen Richard Chalmers Rachel M Elwin Kristin Hunter Paul R |
| author_facet | Bouzid Maha Tyler Kevin M Christen Richard Chalmers Rachel M Elwin Kristin Hunter Paul R |
| author_sort | Bouzid Maha |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Cryptosporidium </it>is a protozoan parasite that causes diarrheal illness in a wide range of hosts including humans. Two species, <it>C. parvum </it>and <it>C. hominis </it>are of primary public health relevance. Genome sequences of these two species are available and show only 3-5% sequence divergence. We investigated this sequence variability, which could correspond either to sequence gaps in the published genome sequences or to the presence of species-specific genes. Comparative genomic tools were used to identify putative species-specific genes and a subset of these genes was tested by PCR in a collection of <it>Cryptosporidium </it>clinical isolates and reference strains.</p> <p>Results</p> <p>The majority of the putative species-specific genes examined were in fact common to <it>C. parvum </it>and <it>C. hominis</it>. PCR product sequence analysis revealed interesting SNPs, the majority of which were species-specific. These genetic loci allowed us to construct a robust and multi-locus analysis. The Neighbour-Joining phylogenetic tree constructed clearly discriminated the previously described lineages of <it>Cryptosporidium </it>species and subtypes.</p> <p>Conclusions</p> <p>Most of the genes identified as being species specific during bioinformatics in <it>Cryptosporidium </it>sp. are in fact present in multiple species and only appear species specific because of gaps in published genome sequences. Nevertheless SNPs may offer a promising approach to studying the taxonomy of closely related species of Cryptosporidia.</p> |
| first_indexed | 2024-12-14T05:31:27Z |
| format | Article |
| id | doaj.art-633e8a09fd3d44d38c2f9fce07f5eb51 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-14T05:31:27Z |
| publishDate | 2010-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-633e8a09fd3d44d38c2f9fce07f5eb512022-12-21T23:15:19ZengBMCBMC Microbiology1471-21802010-08-0110121310.1186/1471-2180-10-213Multi-locus analysis of human infective <it>Cryptosporidium </it>species and subtypes using ten novel genetic lociBouzid MahaTyler Kevin MChristen RichardChalmers Rachel MElwin KristinHunter Paul R<p>Abstract</p> <p>Background</p> <p><it>Cryptosporidium </it>is a protozoan parasite that causes diarrheal illness in a wide range of hosts including humans. Two species, <it>C. parvum </it>and <it>C. hominis </it>are of primary public health relevance. Genome sequences of these two species are available and show only 3-5% sequence divergence. We investigated this sequence variability, which could correspond either to sequence gaps in the published genome sequences or to the presence of species-specific genes. Comparative genomic tools were used to identify putative species-specific genes and a subset of these genes was tested by PCR in a collection of <it>Cryptosporidium </it>clinical isolates and reference strains.</p> <p>Results</p> <p>The majority of the putative species-specific genes examined were in fact common to <it>C. parvum </it>and <it>C. hominis</it>. PCR product sequence analysis revealed interesting SNPs, the majority of which were species-specific. These genetic loci allowed us to construct a robust and multi-locus analysis. The Neighbour-Joining phylogenetic tree constructed clearly discriminated the previously described lineages of <it>Cryptosporidium </it>species and subtypes.</p> <p>Conclusions</p> <p>Most of the genes identified as being species specific during bioinformatics in <it>Cryptosporidium </it>sp. are in fact present in multiple species and only appear species specific because of gaps in published genome sequences. Nevertheless SNPs may offer a promising approach to studying the taxonomy of closely related species of Cryptosporidia.</p>http://www.biomedcentral.com/1471-2180/10/213 |
| spellingShingle | Bouzid Maha Tyler Kevin M Christen Richard Chalmers Rachel M Elwin Kristin Hunter Paul R Multi-locus analysis of human infective <it>Cryptosporidium </it>species and subtypes using ten novel genetic loci BMC Microbiology |
| title | Multi-locus analysis of human infective <it>Cryptosporidium </it>species and subtypes using ten novel genetic loci |
| title_full | Multi-locus analysis of human infective <it>Cryptosporidium </it>species and subtypes using ten novel genetic loci |
| title_fullStr | Multi-locus analysis of human infective <it>Cryptosporidium </it>species and subtypes using ten novel genetic loci |
| title_full_unstemmed | Multi-locus analysis of human infective <it>Cryptosporidium </it>species and subtypes using ten novel genetic loci |
| title_short | Multi-locus analysis of human infective <it>Cryptosporidium </it>species and subtypes using ten novel genetic loci |
| title_sort | multi locus analysis of human infective it cryptosporidium it species and subtypes using ten novel genetic loci |
| url | http://www.biomedcentral.com/1471-2180/10/213 |
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