RETRACTED: A Novel Tumor Suppressor Gene, ZNF24, Inhibits the Development of NSCLC by Inhibiting the WNT Signaling Pathway to Induce Cell Senescence
ObjectiveUnderstanding the characteristics of tumor suppressor genes (TSGs) is of great significance for the development of new targeted treatment strategies for non-small cell lung cancer (NSCLC). Therefore, this present article is to explore the underlying molecular mechanism of ZFN24 inhibiting t...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-07-01
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| Series: | Frontiers in Oncology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2021.664369/full |
| _version_ | 1797248747208441856 |
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| author | Bo Pang Yong Wang Xiaoyan Chang |
| author_facet | Bo Pang Yong Wang Xiaoyan Chang |
| author_sort | Bo Pang |
| collection | DOAJ |
| description | ObjectiveUnderstanding the characteristics of tumor suppressor genes (TSGs) is of great significance for the development of new targeted treatment strategies for non-small cell lung cancer (NSCLC). Therefore, this present article is to explore the underlying molecular mechanism of ZFN24 inhibiting the development of NSCLC.MethodsWe performed RT-PCR and Western blotting for evaluating associated RNA and protein expression. CCK8, colony forming and sphere-forming assays were used to evaluate the proliferation and stemness of NSCLC cells. NSCLC cell senescence was examined by β-galactosidase staining assay. Luciferase assay was performed to evaluate β-catenin transcriptional activity. The effect of ZNF24 on NSCLC cells in vivo was evaluated by the xenograft tumor experiment.ResultsEctopic expression of ZNF24 significantly inhibited cell viability, colony forming ability, and stemness of NSCLC cells. WNT signaling pathway was inhibited by ZNF24 resulting in NSCLC cell senescence. β-catenin transcriptional activity was significantly inhibited by ZNF24 (P < 0.05). Ectopic expression of ZNF24 significantly inhibited xenotransplant tumors growth in vivo (P < 0.05).ConclusionZNF24 could notably inhibit the development of NSCLC by inhibiting the WNT signaling pathway. |
| first_indexed | 2024-04-24T20:19:30Z |
| format | Article |
| id | doaj.art-63414047c66a4516bd26ebcc6fb9e7e6 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-04-24T20:19:30Z |
| publishDate | 2021-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-63414047c66a4516bd26ebcc6fb9e7e62024-03-22T09:10:28ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2021-07-011110.3389/fonc.2021.664369664369RETRACTED: A Novel Tumor Suppressor Gene, ZNF24, Inhibits the Development of NSCLC by Inhibiting the WNT Signaling Pathway to Induce Cell SenescenceBo Pang0Yong Wang1Xiaoyan Chang2Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei, ChinaDepartment of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei, ChinaDepartment of Nephrology, The First Affiliated Hospital of Anhui Medical University, Hefei, ChinaObjectiveUnderstanding the characteristics of tumor suppressor genes (TSGs) is of great significance for the development of new targeted treatment strategies for non-small cell lung cancer (NSCLC). Therefore, this present article is to explore the underlying molecular mechanism of ZFN24 inhibiting the development of NSCLC.MethodsWe performed RT-PCR and Western blotting for evaluating associated RNA and protein expression. CCK8, colony forming and sphere-forming assays were used to evaluate the proliferation and stemness of NSCLC cells. NSCLC cell senescence was examined by β-galactosidase staining assay. Luciferase assay was performed to evaluate β-catenin transcriptional activity. The effect of ZNF24 on NSCLC cells in vivo was evaluated by the xenograft tumor experiment.ResultsEctopic expression of ZNF24 significantly inhibited cell viability, colony forming ability, and stemness of NSCLC cells. WNT signaling pathway was inhibited by ZNF24 resulting in NSCLC cell senescence. β-catenin transcriptional activity was significantly inhibited by ZNF24 (P < 0.05). Ectopic expression of ZNF24 significantly inhibited xenotransplant tumors growth in vivo (P < 0.05).ConclusionZNF24 could notably inhibit the development of NSCLC by inhibiting the WNT signaling pathway.https://www.frontiersin.org/articles/10.3389/fonc.2021.664369/fullZNF24non-small cell lung cancer (NSCLC)WNT signaling pathwaysenescencetumor suppressor genes (TSGs) |
| spellingShingle | Bo Pang Yong Wang Xiaoyan Chang RETRACTED: A Novel Tumor Suppressor Gene, ZNF24, Inhibits the Development of NSCLC by Inhibiting the WNT Signaling Pathway to Induce Cell Senescence Frontiers in Oncology ZNF24 non-small cell lung cancer (NSCLC) WNT signaling pathway senescence tumor suppressor genes (TSGs) |
| title | RETRACTED: A Novel Tumor Suppressor Gene, ZNF24, Inhibits the Development of NSCLC by Inhibiting the WNT Signaling Pathway to Induce Cell Senescence |
| title_full | RETRACTED: A Novel Tumor Suppressor Gene, ZNF24, Inhibits the Development of NSCLC by Inhibiting the WNT Signaling Pathway to Induce Cell Senescence |
| title_fullStr | RETRACTED: A Novel Tumor Suppressor Gene, ZNF24, Inhibits the Development of NSCLC by Inhibiting the WNT Signaling Pathway to Induce Cell Senescence |
| title_full_unstemmed | RETRACTED: A Novel Tumor Suppressor Gene, ZNF24, Inhibits the Development of NSCLC by Inhibiting the WNT Signaling Pathway to Induce Cell Senescence |
| title_short | RETRACTED: A Novel Tumor Suppressor Gene, ZNF24, Inhibits the Development of NSCLC by Inhibiting the WNT Signaling Pathway to Induce Cell Senescence |
| title_sort | retracted a novel tumor suppressor gene znf24 inhibits the development of nsclc by inhibiting the wnt signaling pathway to induce cell senescence |
| topic | ZNF24 non-small cell lung cancer (NSCLC) WNT signaling pathway senescence tumor suppressor genes (TSGs) |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2021.664369/full |
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