Efficient Extraction from Mice Feces for NMR Metabolomics Measurements with Special Emphasis on SCFAs
Nuclear magnetic resonance (NMR) spectroscopy is one of the most promising methods for use in metabolomics studies as it is able to perform non targeted measurement of metabolites in a quantitative and non-destructive way. Sample preparation of liquid samples like urine or blood serum is comparative...
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| Format: | Article |
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MDPI AG
2019-03-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/9/3/55 |
| _version_ | 1811315897796657152 |
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| author | Adrian Hauser Philipp Eisenmann Claudia Muhle-Goll Burkhard Luy Andreas Dötsch Daniela Graf Pavleta Tzvetkova |
| author_facet | Adrian Hauser Philipp Eisenmann Claudia Muhle-Goll Burkhard Luy Andreas Dötsch Daniela Graf Pavleta Tzvetkova |
| author_sort | Adrian Hauser |
| collection | DOAJ |
| description | Nuclear magnetic resonance (NMR) spectroscopy is one of the most promising methods for use in metabolomics studies as it is able to perform non targeted measurement of metabolites in a quantitative and non-destructive way. Sample preparation of liquid samples like urine or blood serum is comparatively easy in NMR metabolomics, because mainly buffer and chemical shift reference substance are added. For solid samples like feces suitable extraction protocols need to be defined as initial step, where the exact protocol depends on sample type and features. Focusing on short chain fatty acids (SCFAs) in mice feces, we describe here a set of extraction protocols developed with the aim to suppress changes in metabolite composition within 24 h after extraction. Feces are obtained from mice fed on either standard rodent diet or high fat diet. The protocols presented in this manuscript are straightforward for application, and successfully minimize residual bacterial and enzymatic activities. Additionally, they are able to minimize the lipid background originating from the high fat diet. |
| first_indexed | 2024-04-13T11:39:46Z |
| format | Article |
| id | doaj.art-63447a3fde4d42019ac097b36588068a |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-04-13T11:39:46Z |
| publishDate | 2019-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-63447a3fde4d42019ac097b36588068a2022-12-22T02:48:20ZengMDPI AGMetabolites2218-19892019-03-01935510.3390/metabo9030055metabo9030055Efficient Extraction from Mice Feces for NMR Metabolomics Measurements with Special Emphasis on SCFAsAdrian Hauser0Philipp Eisenmann1Claudia Muhle-Goll2Burkhard Luy3Andreas Dötsch4Daniela Graf5Pavleta Tzvetkova6Karlsruhe Institute of Technology, Institute of Organic Chemistry, Fritz-Haber Weg 6, 76131 Karlsruhe, GermanyKarlsruhe Institute of Technology, Institute of Organic Chemistry, Fritz-Haber Weg 6, 76131 Karlsruhe, GermanyKarlsruhe Institute of Technology, Institute for Biological Interfaces-4, Magnetic Resonance, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, GermanyKarlsruhe Institute of Technology, Institute of Organic Chemistry, Fritz-Haber Weg 6, 76131 Karlsruhe, GermanyMax Rubner-Institut, Haid-und-Neu-Straße 9, 76131 Karlsruhe, GermanyMax Rubner-Institut, Haid-und-Neu-Straße 9, 76131 Karlsruhe, GermanyKarlsruhe Institute of Technology, Institute for Biological Interfaces-4, Magnetic Resonance, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, GermanyNuclear magnetic resonance (NMR) spectroscopy is one of the most promising methods for use in metabolomics studies as it is able to perform non targeted measurement of metabolites in a quantitative and non-destructive way. Sample preparation of liquid samples like urine or blood serum is comparatively easy in NMR metabolomics, because mainly buffer and chemical shift reference substance are added. For solid samples like feces suitable extraction protocols need to be defined as initial step, where the exact protocol depends on sample type and features. Focusing on short chain fatty acids (SCFAs) in mice feces, we describe here a set of extraction protocols developed with the aim to suppress changes in metabolite composition within 24 h after extraction. Feces are obtained from mice fed on either standard rodent diet or high fat diet. The protocols presented in this manuscript are straightforward for application, and successfully minimize residual bacterial and enzymatic activities. Additionally, they are able to minimize the lipid background originating from the high fat diet.https://www.mdpi.com/2218-1989/9/3/55metabolomicsmicefecesfaecesNMR spectroscopyshort chain fatty acidsdietary fibersextraction protocoldietNMRextraction |
| spellingShingle | Adrian Hauser Philipp Eisenmann Claudia Muhle-Goll Burkhard Luy Andreas Dötsch Daniela Graf Pavleta Tzvetkova Efficient Extraction from Mice Feces for NMR Metabolomics Measurements with Special Emphasis on SCFAs Metabolites metabolomics mice feces faeces NMR spectroscopy short chain fatty acids dietary fibers extraction protocol diet NMR extraction |
| title | Efficient Extraction from Mice Feces for NMR Metabolomics Measurements with Special Emphasis on SCFAs |
| title_full | Efficient Extraction from Mice Feces for NMR Metabolomics Measurements with Special Emphasis on SCFAs |
| title_fullStr | Efficient Extraction from Mice Feces for NMR Metabolomics Measurements with Special Emphasis on SCFAs |
| title_full_unstemmed | Efficient Extraction from Mice Feces for NMR Metabolomics Measurements with Special Emphasis on SCFAs |
| title_short | Efficient Extraction from Mice Feces for NMR Metabolomics Measurements with Special Emphasis on SCFAs |
| title_sort | efficient extraction from mice feces for nmr metabolomics measurements with special emphasis on scfas |
| topic | metabolomics mice feces faeces NMR spectroscopy short chain fatty acids dietary fibers extraction protocol diet NMR extraction |
| url | https://www.mdpi.com/2218-1989/9/3/55 |
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