Grape powder intake prevents ovariectomy-induced anxiety-like behavior, memory impairment and high blood pressure in female Wistar rats.
Diminished estrogen influence at menopause is reported to be associated with cognitive decline, heightened anxiety and hypertension. While estrogen therapy is often prescribed to overcome these behavioral and physiological deficits, antioxidants which have been shown beneficial are gaining nutrition...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3767647?pdf=render |
| _version_ | 1818217138690719744 |
|---|---|
| author | Gaurav Patki Farida H Allam Fatin Atrooz An T Dao Naimesh Solanki Gaurav Chugh Mohammad Asghar Faizan Jafri Ritu Bohat Karim A Alkadhi Samina Salim |
| author_facet | Gaurav Patki Farida H Allam Fatin Atrooz An T Dao Naimesh Solanki Gaurav Chugh Mohammad Asghar Faizan Jafri Ritu Bohat Karim A Alkadhi Samina Salim |
| author_sort | Gaurav Patki |
| collection | DOAJ |
| description | Diminished estrogen influence at menopause is reported to be associated with cognitive decline, heightened anxiety and hypertension. While estrogen therapy is often prescribed to overcome these behavioral and physiological deficits, antioxidants which have been shown beneficial are gaining nutritional intervention and popularity. Therefore, in the present study, utilizing the antioxidant properties of grapes, we have examined effect of 3 weeks of grape powder (GP; 15 g/L dissolved in tap water) treatment on anxiety-like behavior, learning-memory impairment and high blood pressure in ovariectomized (OVX) rats. Four groups of female Wistar rats were used; sham control, sham-GP treated, OVX and OVX+GP treated. We observed a significant increase in systolic and diastolic blood pressure in OVX rats as compared to sham-controls. Furthermore, ovariectomy increased anxiety-like behavior and caused learning and memory impairment in rats as compared to sham-controls. Interestingly, providing grape powder treated water to OVX rats restored both systolic and diastolic blood pressure, decreased anxiety-like behavior and improved memory function. Moreover, OVX rats exhibited an impaired long term potentiation which was restored with grape powder treatment. Furthermore, ovariectomy increased oxidative stress in the brain, serum and urine, selectively decreasing antioxidant enzyme, glyoxalase-1 protein expression in the hippocampus but not in the cortex and amygdala of OVX rats, while grape powder treatment reversed these effects. Other antioxidant enzyme levels, including manganese superoxide dismutase (SOD) and Cu/Zn SOD remained unchanged. We suggest that grape powder by regulating oxidative stress mechanisms exerts its protective effect on blood pressure, learning-memory and anxiety-like behavior. Our study is the first to examine behavioral, biochemical, physiological and electrophysiological outcome of estrogen depletion in rats and to test protective role of grape powder, all in the same study. |
| first_indexed | 2024-12-12T07:03:06Z |
| format | Article |
| id | doaj.art-63457f1773ee4b6db979ab42052604de |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T07:03:06Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-63457f1773ee4b6db979ab42052604de2022-12-22T00:33:48ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0189e7452210.1371/journal.pone.0074522Grape powder intake prevents ovariectomy-induced anxiety-like behavior, memory impairment and high blood pressure in female Wistar rats.Gaurav PatkiFarida H AllamFatin AtroozAn T DaoNaimesh SolankiGaurav ChughMohammad AsgharFaizan JafriRitu BohatKarim A AlkadhiSamina SalimDiminished estrogen influence at menopause is reported to be associated with cognitive decline, heightened anxiety and hypertension. While estrogen therapy is often prescribed to overcome these behavioral and physiological deficits, antioxidants which have been shown beneficial are gaining nutritional intervention and popularity. Therefore, in the present study, utilizing the antioxidant properties of grapes, we have examined effect of 3 weeks of grape powder (GP; 15 g/L dissolved in tap water) treatment on anxiety-like behavior, learning-memory impairment and high blood pressure in ovariectomized (OVX) rats. Four groups of female Wistar rats were used; sham control, sham-GP treated, OVX and OVX+GP treated. We observed a significant increase in systolic and diastolic blood pressure in OVX rats as compared to sham-controls. Furthermore, ovariectomy increased anxiety-like behavior and caused learning and memory impairment in rats as compared to sham-controls. Interestingly, providing grape powder treated water to OVX rats restored both systolic and diastolic blood pressure, decreased anxiety-like behavior and improved memory function. Moreover, OVX rats exhibited an impaired long term potentiation which was restored with grape powder treatment. Furthermore, ovariectomy increased oxidative stress in the brain, serum and urine, selectively decreasing antioxidant enzyme, glyoxalase-1 protein expression in the hippocampus but not in the cortex and amygdala of OVX rats, while grape powder treatment reversed these effects. Other antioxidant enzyme levels, including manganese superoxide dismutase (SOD) and Cu/Zn SOD remained unchanged. We suggest that grape powder by regulating oxidative stress mechanisms exerts its protective effect on blood pressure, learning-memory and anxiety-like behavior. Our study is the first to examine behavioral, biochemical, physiological and electrophysiological outcome of estrogen depletion in rats and to test protective role of grape powder, all in the same study.http://europepmc.org/articles/PMC3767647?pdf=render |
| spellingShingle | Gaurav Patki Farida H Allam Fatin Atrooz An T Dao Naimesh Solanki Gaurav Chugh Mohammad Asghar Faizan Jafri Ritu Bohat Karim A Alkadhi Samina Salim Grape powder intake prevents ovariectomy-induced anxiety-like behavior, memory impairment and high blood pressure in female Wistar rats. PLoS ONE |
| title | Grape powder intake prevents ovariectomy-induced anxiety-like behavior, memory impairment and high blood pressure in female Wistar rats. |
| title_full | Grape powder intake prevents ovariectomy-induced anxiety-like behavior, memory impairment and high blood pressure in female Wistar rats. |
| title_fullStr | Grape powder intake prevents ovariectomy-induced anxiety-like behavior, memory impairment and high blood pressure in female Wistar rats. |
| title_full_unstemmed | Grape powder intake prevents ovariectomy-induced anxiety-like behavior, memory impairment and high blood pressure in female Wistar rats. |
| title_short | Grape powder intake prevents ovariectomy-induced anxiety-like behavior, memory impairment and high blood pressure in female Wistar rats. |
| title_sort | grape powder intake prevents ovariectomy induced anxiety like behavior memory impairment and high blood pressure in female wistar rats |
| url | http://europepmc.org/articles/PMC3767647?pdf=render |
| work_keys_str_mv | AT gauravpatki grapepowderintakepreventsovariectomyinducedanxietylikebehaviormemoryimpairmentandhighbloodpressureinfemalewistarrats AT faridahallam grapepowderintakepreventsovariectomyinducedanxietylikebehaviormemoryimpairmentandhighbloodpressureinfemalewistarrats AT fatinatrooz grapepowderintakepreventsovariectomyinducedanxietylikebehaviormemoryimpairmentandhighbloodpressureinfemalewistarrats AT antdao grapepowderintakepreventsovariectomyinducedanxietylikebehaviormemoryimpairmentandhighbloodpressureinfemalewistarrats AT naimeshsolanki grapepowderintakepreventsovariectomyinducedanxietylikebehaviormemoryimpairmentandhighbloodpressureinfemalewistarrats AT gauravchugh grapepowderintakepreventsovariectomyinducedanxietylikebehaviormemoryimpairmentandhighbloodpressureinfemalewistarrats AT mohammadasghar grapepowderintakepreventsovariectomyinducedanxietylikebehaviormemoryimpairmentandhighbloodpressureinfemalewistarrats AT faizanjafri grapepowderintakepreventsovariectomyinducedanxietylikebehaviormemoryimpairmentandhighbloodpressureinfemalewistarrats AT ritubohat grapepowderintakepreventsovariectomyinducedanxietylikebehaviormemoryimpairmentandhighbloodpressureinfemalewistarrats AT karimaalkadhi grapepowderintakepreventsovariectomyinducedanxietylikebehaviormemoryimpairmentandhighbloodpressureinfemalewistarrats AT saminasalim grapepowderintakepreventsovariectomyinducedanxietylikebehaviormemoryimpairmentandhighbloodpressureinfemalewistarrats |