Proliferating Astrocytes in Primary Culture Do Not Depend upon Mitochondrial Respiratory Complex I Activity or Oxidative Phosphorylation
Understanding the role of astrocytes in the development of the nervous system and neurodegenerative disorders implies a necessary knowledge of the oxidative metabolism of proliferating astrocytes. The electron flux through mitochondrial respiratory complexes and oxidative phosphorylation may impact...
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MDPI AG
2023-02-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/12/5/683 |
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| author | Ellen A. Silva Ana P. Dalla Costa Juliana S. Ruas Edilene S. Siqueira-Santos Annelise Francisco Roger F. Castilho |
| author_facet | Ellen A. Silva Ana P. Dalla Costa Juliana S. Ruas Edilene S. Siqueira-Santos Annelise Francisco Roger F. Castilho |
| author_sort | Ellen A. Silva |
| collection | DOAJ |
| description | Understanding the role of astrocytes in the development of the nervous system and neurodegenerative disorders implies a necessary knowledge of the oxidative metabolism of proliferating astrocytes. The electron flux through mitochondrial respiratory complexes and oxidative phosphorylation may impact the growth and viability of these astrocytes. Here, we aimed at assessing to which extent mitochondrial oxidative metabolism is required for astrocyte survival and proliferation. Primary astrocytes from the neonatal mouse cortex were cultured in a physiologically relevant medium with the addition of piericidin A or oligomycin at concentrations that fully inhibit complex I-linked respiration and ATP synthase, respectively. The presence of these mitochondrial inhibitors for up to 6 days in a culture medium elicited only minor effects on astrocyte growth. Moreover, neither the morphology nor the proportion of glial fibrillary acidic protein-positive astrocytes in culture was affected by piericidin A or oligomycin. Metabolic characterization of the astrocytes showed a relevant glycolytic metabolism under basal conditions, despite functional oxidative phosphorylation and large spare respiratory capacity. Our data suggest that astrocytes in primary culture can sustainably proliferate when their energy metabolism relies only on aerobic glycolysis since their growth and survival do not require electron flux through respiratory complex I or oxidative phosphorylation. |
| first_indexed | 2024-03-11T07:28:40Z |
| format | Article |
| id | doaj.art-6347a069b7364f418781bbc7b25408f3 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-11T07:28:40Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-6347a069b7364f418781bbc7b25408f32023-11-17T07:26:47ZengMDPI AGCells2073-44092023-02-0112568310.3390/cells12050683Proliferating Astrocytes in Primary Culture Do Not Depend upon Mitochondrial Respiratory Complex I Activity or Oxidative PhosphorylationEllen A. Silva0Ana P. Dalla Costa1Juliana S. Ruas2Edilene S. Siqueira-Santos3Annelise Francisco4Roger F. Castilho5Department of Pathology, School of Medical Sciences, State University of Campinas (UNICAMP), Campinas 13083-887, SP, BrazilDepartment of Pathology, School of Medical Sciences, State University of Campinas (UNICAMP), Campinas 13083-887, SP, BrazilDepartment of Pathology, School of Medical Sciences, State University of Campinas (UNICAMP), Campinas 13083-887, SP, BrazilDepartment of Pathology, School of Medical Sciences, State University of Campinas (UNICAMP), Campinas 13083-887, SP, BrazilDepartment of Pathology, School of Medical Sciences, State University of Campinas (UNICAMP), Campinas 13083-887, SP, BrazilDepartment of Pathology, School of Medical Sciences, State University of Campinas (UNICAMP), Campinas 13083-887, SP, BrazilUnderstanding the role of astrocytes in the development of the nervous system and neurodegenerative disorders implies a necessary knowledge of the oxidative metabolism of proliferating astrocytes. The electron flux through mitochondrial respiratory complexes and oxidative phosphorylation may impact the growth and viability of these astrocytes. Here, we aimed at assessing to which extent mitochondrial oxidative metabolism is required for astrocyte survival and proliferation. Primary astrocytes from the neonatal mouse cortex were cultured in a physiologically relevant medium with the addition of piericidin A or oligomycin at concentrations that fully inhibit complex I-linked respiration and ATP synthase, respectively. The presence of these mitochondrial inhibitors for up to 6 days in a culture medium elicited only minor effects on astrocyte growth. Moreover, neither the morphology nor the proportion of glial fibrillary acidic protein-positive astrocytes in culture was affected by piericidin A or oligomycin. Metabolic characterization of the astrocytes showed a relevant glycolytic metabolism under basal conditions, despite functional oxidative phosphorylation and large spare respiratory capacity. Our data suggest that astrocytes in primary culture can sustainably proliferate when their energy metabolism relies only on aerobic glycolysis since their growth and survival do not require electron flux through respiratory complex I or oxidative phosphorylation.https://www.mdpi.com/2073-4409/12/5/683astrocytesbioenergeticmitochondriaoligomycinOXPHOSpiericidin A |
| spellingShingle | Ellen A. Silva Ana P. Dalla Costa Juliana S. Ruas Edilene S. Siqueira-Santos Annelise Francisco Roger F. Castilho Proliferating Astrocytes in Primary Culture Do Not Depend upon Mitochondrial Respiratory Complex I Activity or Oxidative Phosphorylation Cells astrocytes bioenergetic mitochondria oligomycin OXPHOS piericidin A |
| title | Proliferating Astrocytes in Primary Culture Do Not Depend upon Mitochondrial Respiratory Complex I Activity or Oxidative Phosphorylation |
| title_full | Proliferating Astrocytes in Primary Culture Do Not Depend upon Mitochondrial Respiratory Complex I Activity or Oxidative Phosphorylation |
| title_fullStr | Proliferating Astrocytes in Primary Culture Do Not Depend upon Mitochondrial Respiratory Complex I Activity or Oxidative Phosphorylation |
| title_full_unstemmed | Proliferating Astrocytes in Primary Culture Do Not Depend upon Mitochondrial Respiratory Complex I Activity or Oxidative Phosphorylation |
| title_short | Proliferating Astrocytes in Primary Culture Do Not Depend upon Mitochondrial Respiratory Complex I Activity or Oxidative Phosphorylation |
| title_sort | proliferating astrocytes in primary culture do not depend upon mitochondrial respiratory complex i activity or oxidative phosphorylation |
| topic | astrocytes bioenergetic mitochondria oligomycin OXPHOS piericidin A |
| url | https://www.mdpi.com/2073-4409/12/5/683 |
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