Chronic Stress Triggers Expression of Immediate Early Genes and Differentially Affects the Expression of AMPA and NMDA Subunits in Dorsal and Ventral Hippocampus of Rats
Previous studies in rats have demonstrated that chronic restraint stress triggers anhedonia, depressive-like behaviors, anxiety and a reduction in dendritic spine density in hippocampal neurons. In this study, we compared the effect of repeated stress on the expression of α-amino-3-hydroxy-5-methyl-...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-08-01
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| Series: | Frontiers in Molecular Neuroscience |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fnmol.2017.00244/full |
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| author | Anibal Pacheco Felipe I. Aguayo Esteban Aliaga Mauricio Muñoz Gonzalo García-Rojo Felipe A. Olave Nicolas A. Parra-Fiedler Alexandra García-Pérez Macarena Tejos-Bravo Paulina S. Rojas Claudio S. Parra Jenny L. Fiedler |
| author_facet | Anibal Pacheco Felipe I. Aguayo Esteban Aliaga Mauricio Muñoz Gonzalo García-Rojo Felipe A. Olave Nicolas A. Parra-Fiedler Alexandra García-Pérez Macarena Tejos-Bravo Paulina S. Rojas Claudio S. Parra Jenny L. Fiedler |
| author_sort | Anibal Pacheco |
| collection | DOAJ |
| description | Previous studies in rats have demonstrated that chronic restraint stress triggers anhedonia, depressive-like behaviors, anxiety and a reduction in dendritic spine density in hippocampal neurons. In this study, we compared the effect of repeated stress on the expression of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) and N-methyl-D-aspartate (NMDA) receptor subunits in dorsal and ventral hippocampus (VH). Adult male Sprague-Dawley rats were randomly divided into control and stressed groups, and were daily restrained in their motion (2.5 h/day) during 14 days. We found that chronic stress promotes an increase in c-Fos mRNA levels in both hippocampal areas, although it was observed a reduction in the immunoreactivity at pyramidal cell layer. Furthermore, Arc mRNAs levels were increased in both dorsal and VH, accompanied by an increase in Arc immunoreactivity in dendritic hippocampal layers. Furthermore, stress triggered a reduction in PSD-95 and NR1 protein levels in whole extract of dorsal and VH. Moreover, a reduction in NR2A/NR2B ratio was observed only in dorsal pole. In synaptosomal fractions, we detected a rise in NR1 in dorsal hippocampus (DH). By indirect immunofluorescence we found that NR1 subunits rise, especially in neuropil areas of dorsal, but not VH. In relation to AMPA receptor (AMPAR) subunits, chronic stress did not trigger any change, either in dorsal or ventral hippocampal areas. These data suggest that DH is more sensitive than VH to chronic stress exposure, mainly altering the expression of NMDA receptor (NMDAR) subunits, and probably favors changes in the configuration of this receptor that may influence the function of this area. |
| first_indexed | 2024-12-10T22:45:57Z |
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| institution | Directory Open Access Journal |
| issn | 1662-5099 |
| language | English |
| last_indexed | 2024-12-10T22:45:57Z |
| publishDate | 2017-08-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Molecular Neuroscience |
| spelling | doaj.art-634acb32072f46788268a17020dfb4e62022-12-22T01:30:34ZengFrontiers Media S.A.Frontiers in Molecular Neuroscience1662-50992017-08-011010.3389/fnmol.2017.00244274093Chronic Stress Triggers Expression of Immediate Early Genes and Differentially Affects the Expression of AMPA and NMDA Subunits in Dorsal and Ventral Hippocampus of RatsAnibal Pacheco0Felipe I. Aguayo1Esteban Aliaga2Mauricio Muñoz3Gonzalo García-Rojo4Felipe A. Olave5Nicolas A. Parra-Fiedler6Alexandra García-Pérez7Macarena Tejos-Bravo8Paulina S. Rojas9Claudio S. Parra10Jenny L. Fiedler11Laboratory of Neuroplasticity and Neurogenetics, Faculty of Chemical and Pharmaceutical Sciences, Department of Biochemistry and Molecular Biology, Universidad de ChileIndependencia, ChileLaboratory of Neuroplasticity and Neurogenetics, Faculty of Chemical and Pharmaceutical Sciences, Department of Biochemistry and Molecular Biology, Universidad de ChileIndependencia, ChileDepartment of Kinesiology, Faculty of Health Sciences, Universidad Católica del MauleTalca, ChileLaboratory of Neuroplasticity and Neurogenetics, Faculty of Chemical and Pharmaceutical Sciences, Department of Biochemistry and Molecular Biology, Universidad de ChileIndependencia, ChileLaboratory of Neuroplasticity and Neurogenetics, Faculty of Chemical and Pharmaceutical Sciences, Department of Biochemistry and Molecular Biology, Universidad de ChileIndependencia, ChileLaboratory of Neuroplasticity and Neurogenetics, Faculty of Chemical and Pharmaceutical Sciences, Department of Biochemistry and Molecular Biology, Universidad de ChileIndependencia, ChileLaboratory of Neuroplasticity and Neurogenetics, Faculty of Chemical and Pharmaceutical Sciences, Department of Biochemistry and Molecular Biology, Universidad de ChileIndependencia, ChileLaboratory of Neuroplasticity and Neurogenetics, Faculty of Chemical and Pharmaceutical Sciences, Department of Biochemistry and Molecular Biology, Universidad de ChileIndependencia, ChileLaboratory of Neuroplasticity and Neurogenetics, Faculty of Chemical and Pharmaceutical Sciences, Department of Biochemistry and Molecular Biology, Universidad de ChileIndependencia, ChileFaculty of Medicine, School of Pharmacy, Universidad Andres BelloSantiago, ChileLaboratory of Neuroplasticity and Neurogenetics, Faculty of Chemical and Pharmaceutical Sciences, Department of Biochemistry and Molecular Biology, Universidad de ChileIndependencia, ChileLaboratory of Neuroplasticity and Neurogenetics, Faculty of Chemical and Pharmaceutical Sciences, Department of Biochemistry and Molecular Biology, Universidad de ChileIndependencia, ChilePrevious studies in rats have demonstrated that chronic restraint stress triggers anhedonia, depressive-like behaviors, anxiety and a reduction in dendritic spine density in hippocampal neurons. In this study, we compared the effect of repeated stress on the expression of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) and N-methyl-D-aspartate (NMDA) receptor subunits in dorsal and ventral hippocampus (VH). Adult male Sprague-Dawley rats were randomly divided into control and stressed groups, and were daily restrained in their motion (2.5 h/day) during 14 days. We found that chronic stress promotes an increase in c-Fos mRNA levels in both hippocampal areas, although it was observed a reduction in the immunoreactivity at pyramidal cell layer. Furthermore, Arc mRNAs levels were increased in both dorsal and VH, accompanied by an increase in Arc immunoreactivity in dendritic hippocampal layers. Furthermore, stress triggered a reduction in PSD-95 and NR1 protein levels in whole extract of dorsal and VH. Moreover, a reduction in NR2A/NR2B ratio was observed only in dorsal pole. In synaptosomal fractions, we detected a rise in NR1 in dorsal hippocampus (DH). By indirect immunofluorescence we found that NR1 subunits rise, especially in neuropil areas of dorsal, but not VH. In relation to AMPA receptor (AMPAR) subunits, chronic stress did not trigger any change, either in dorsal or ventral hippocampal areas. These data suggest that DH is more sensitive than VH to chronic stress exposure, mainly altering the expression of NMDA receptor (NMDAR) subunits, and probably favors changes in the configuration of this receptor that may influence the function of this area.http://journal.frontiersin.org/article/10.3389/fnmol.2017.00244/fullstressdorsal hippocampusventral hippocampusNMDA receptor subunitsAMPA receptor subunitsimmediately early genes |
| spellingShingle | Anibal Pacheco Felipe I. Aguayo Esteban Aliaga Mauricio Muñoz Gonzalo García-Rojo Felipe A. Olave Nicolas A. Parra-Fiedler Alexandra García-Pérez Macarena Tejos-Bravo Paulina S. Rojas Claudio S. Parra Jenny L. Fiedler Chronic Stress Triggers Expression of Immediate Early Genes and Differentially Affects the Expression of AMPA and NMDA Subunits in Dorsal and Ventral Hippocampus of Rats Frontiers in Molecular Neuroscience stress dorsal hippocampus ventral hippocampus NMDA receptor subunits AMPA receptor subunits immediately early genes |
| title | Chronic Stress Triggers Expression of Immediate Early Genes and Differentially Affects the Expression of AMPA and NMDA Subunits in Dorsal and Ventral Hippocampus of Rats |
| title_full | Chronic Stress Triggers Expression of Immediate Early Genes and Differentially Affects the Expression of AMPA and NMDA Subunits in Dorsal and Ventral Hippocampus of Rats |
| title_fullStr | Chronic Stress Triggers Expression of Immediate Early Genes and Differentially Affects the Expression of AMPA and NMDA Subunits in Dorsal and Ventral Hippocampus of Rats |
| title_full_unstemmed | Chronic Stress Triggers Expression of Immediate Early Genes and Differentially Affects the Expression of AMPA and NMDA Subunits in Dorsal and Ventral Hippocampus of Rats |
| title_short | Chronic Stress Triggers Expression of Immediate Early Genes and Differentially Affects the Expression of AMPA and NMDA Subunits in Dorsal and Ventral Hippocampus of Rats |
| title_sort | chronic stress triggers expression of immediate early genes and differentially affects the expression of ampa and nmda subunits in dorsal and ventral hippocampus of rats |
| topic | stress dorsal hippocampus ventral hippocampus NMDA receptor subunits AMPA receptor subunits immediately early genes |
| url | http://journal.frontiersin.org/article/10.3389/fnmol.2017.00244/full |
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