Molecular Characterization and Phylogenetic Congruence of Hydropsyche sciligra (Tricoptera: Hydropsychidae) Using Mitochondrial and Nuclear Markers
Background: Caddisflies have significant roles in freshwater ecosystems. Morphological identification is the major impediment in accurate species identification of Hydropsychids. Mitochondrial and nuclear markers are suitable for molecular systematics of these group of arthropods. Methods: Trichopte...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2017-04-01
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| Series: | Journal of Arthropod-Borne Diseases |
| Subjects: | |
| Online Access: | https://jad.tums.ac.ir/index.php/jad/article/view/429 |
| _version_ | 1818390597575114752 |
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| author | Naseh Maleki-Ravasan Abbas Bahrami Hassan Vatandoost Mansoureh Shayeghi Mona Koosha Mohammad Ali Oshaghi |
| author_facet | Naseh Maleki-Ravasan Abbas Bahrami Hassan Vatandoost Mansoureh Shayeghi Mona Koosha Mohammad Ali Oshaghi |
| author_sort | Naseh Maleki-Ravasan |
| collection | DOAJ |
| description | Background: Caddisflies have significant roles in freshwater ecosystems. Morphological identification is the major impediment in accurate species identification of Hydropsychids. Mitochondrial and nuclear markers are suitable for molecular systematics of these group of arthropods.
Methods: Trichopteran specimens of Lavasan District in northeastern Tehran, Iran were collected in 2012, and described using the morphological and molecular characters of mitochondrial cytochrome c oxidase subunit I (mt-COI) and three expansion fragments of large subunit (LSU) nuclear ribosomal DNA (28S rDNA) D1, D2, and D3. The resemblance of the specimen sequences was obtained by conducting BLAST searches against the GenBank database and by using simple maximum likelihood clustering using COI, D1, D2, D3, and combination of D1-D2-D3 sequence data sets.
Results: Based on morphological traits the specimens were resembled to Hydropsyche sciligra however there were no its counterpart sequences in the GenBank. Due to lack of unique group of data set for each gene fragment, the specimens were associated with different taxa on molecular phylograms. The sequence contents of the COI, D1, D2, D3, and D1-D3 regions clustered H. sciligra with H. brevis, H. angustipennis, H. occidentalis, H. hedini, H. grahami, and H. longifurca/H. naumanni, respectively.
Conclusion: Phylogenies obtained from combination of D1-D3 showed the highest bootstrap values for most of clades suggesting that long LSU-rDNA potentially is more useful for understanding phylogenetic relationships of caddisflies. A large-scale molecular and zoogeographic study on trichopteran species is suggested to revise and to develop the current knowledge of the caddisfly fauna and distributions in the country. |
| first_indexed | 2024-12-14T05:00:10Z |
| format | Article |
| id | doaj.art-634e978ea7d64e0baa07a097bf7920c0 |
| institution | Directory Open Access Journal |
| issn | 1735-7179 2322-2271 |
| language | English |
| last_indexed | 2024-12-14T05:00:10Z |
| publishDate | 2017-04-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Journal of Arthropod-Borne Diseases |
| spelling | doaj.art-634e978ea7d64e0baa07a097bf7920c02022-12-21T23:16:15ZengTehran University of Medical SciencesJournal of Arthropod-Borne Diseases1735-71792322-22712017-04-01111314Molecular Characterization and Phylogenetic Congruence of Hydropsyche sciligra (Tricoptera: Hydropsychidae) Using Mitochondrial and Nuclear MarkersNaseh Maleki-Ravasan0Abbas Bahrami1Hassan Vatandoost2Mansoureh Shayeghi3Mona Koosha4Mohammad Ali Oshaghi5Malaria and Vector Research Group (MVRG), Biotechnology Research Center (BRC), Pasteur Institute of Iran (PII), Tehran, IranDepartment of Medical Parasitology and Mycology, Faculty of Medicine, Alborz University of Medical Sciences, Alborz Province, Karaj, Iran Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, IranBackground: Caddisflies have significant roles in freshwater ecosystems. Morphological identification is the major impediment in accurate species identification of Hydropsychids. Mitochondrial and nuclear markers are suitable for molecular systematics of these group of arthropods. Methods: Trichopteran specimens of Lavasan District in northeastern Tehran, Iran were collected in 2012, and described using the morphological and molecular characters of mitochondrial cytochrome c oxidase subunit I (mt-COI) and three expansion fragments of large subunit (LSU) nuclear ribosomal DNA (28S rDNA) D1, D2, and D3. The resemblance of the specimen sequences was obtained by conducting BLAST searches against the GenBank database and by using simple maximum likelihood clustering using COI, D1, D2, D3, and combination of D1-D2-D3 sequence data sets. Results: Based on morphological traits the specimens were resembled to Hydropsyche sciligra however there were no its counterpart sequences in the GenBank. Due to lack of unique group of data set for each gene fragment, the specimens were associated with different taxa on molecular phylograms. The sequence contents of the COI, D1, D2, D3, and D1-D3 regions clustered H. sciligra with H. brevis, H. angustipennis, H. occidentalis, H. hedini, H. grahami, and H. longifurca/H. naumanni, respectively. Conclusion: Phylogenies obtained from combination of D1-D3 showed the highest bootstrap values for most of clades suggesting that long LSU-rDNA potentially is more useful for understanding phylogenetic relationships of caddisflies. A large-scale molecular and zoogeographic study on trichopteran species is suggested to revise and to develop the current knowledge of the caddisfly fauna and distributions in the country.https://jad.tums.ac.ir/index.php/jad/article/view/429CaddisfliesHydropsyche sciligraCOILSU rDNAMolecular systematicsIran |
| spellingShingle | Naseh Maleki-Ravasan Abbas Bahrami Hassan Vatandoost Mansoureh Shayeghi Mona Koosha Mohammad Ali Oshaghi Molecular Characterization and Phylogenetic Congruence of Hydropsyche sciligra (Tricoptera: Hydropsychidae) Using Mitochondrial and Nuclear Markers Journal of Arthropod-Borne Diseases Caddisflies Hydropsyche sciligra COI LSU rDNA Molecular systematics Iran |
| title | Molecular Characterization and Phylogenetic Congruence of Hydropsyche sciligra (Tricoptera: Hydropsychidae) Using Mitochondrial and Nuclear Markers |
| title_full | Molecular Characterization and Phylogenetic Congruence of Hydropsyche sciligra (Tricoptera: Hydropsychidae) Using Mitochondrial and Nuclear Markers |
| title_fullStr | Molecular Characterization and Phylogenetic Congruence of Hydropsyche sciligra (Tricoptera: Hydropsychidae) Using Mitochondrial and Nuclear Markers |
| title_full_unstemmed | Molecular Characterization and Phylogenetic Congruence of Hydropsyche sciligra (Tricoptera: Hydropsychidae) Using Mitochondrial and Nuclear Markers |
| title_short | Molecular Characterization and Phylogenetic Congruence of Hydropsyche sciligra (Tricoptera: Hydropsychidae) Using Mitochondrial and Nuclear Markers |
| title_sort | molecular characterization and phylogenetic congruence of hydropsyche sciligra tricoptera hydropsychidae using mitochondrial and nuclear markers |
| topic | Caddisflies Hydropsyche sciligra COI LSU rDNA Molecular systematics Iran |
| url | https://jad.tums.ac.ir/index.php/jad/article/view/429 |
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