Quantitative measurement of diffusion-weighted imaging signal using expression-controlled aquaporin-4 cells: Comparative study of 2-compartment and diffusion kurtosis imaging models.
The purpose of this study was to compare parameter estimates for the 2-compartment and diffusion kurtosis imaging models obtained from diffusion-weighted imaging (DWI) of aquaporin-4 (AQP4) expression-controlled cells, and to look for biomarkers that indicate differences in the cell membrane water p...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0266465 |
| _version_ | 1811296568679071744 |
|---|---|
| author | Akiko Imaizumi Takayuki Obata Jeff Kershaw Yasuhiko Tachibana Yoichiro Abe Sayaka Shibata Nobuhiro Nitta Ichio Aoki Masato Yasui Tatsuya Higashi |
| author_facet | Akiko Imaizumi Takayuki Obata Jeff Kershaw Yasuhiko Tachibana Yoichiro Abe Sayaka Shibata Nobuhiro Nitta Ichio Aoki Masato Yasui Tatsuya Higashi |
| author_sort | Akiko Imaizumi |
| collection | DOAJ |
| description | The purpose of this study was to compare parameter estimates for the 2-compartment and diffusion kurtosis imaging models obtained from diffusion-weighted imaging (DWI) of aquaporin-4 (AQP4) expression-controlled cells, and to look for biomarkers that indicate differences in the cell membrane water permeability. DWI was performed on AQP4-expressing and non-expressing cells and the signal was analyzed with the 2-compartment and diffusion kurtosis imaging models. For the 2-compartment model, the diffusion coefficients (Df, Ds) and volume fractions (Ff, Fs, Ff = 1-Fs) of the fast and slow compartments were estimated. For the diffusion kurtosis imaging model, estimates of the diffusion kurtosis (K) and corrected diffusion coefficient (D) were obtained. For the 2-compartment model, Ds and Fs showed clear differences between AQP4-expressing and non-expressing cells. Fs was also sensitive to cell density. There was no clear relationship with the cell type for the diffusion kurtosis imaging model parameters. Changes to cell membrane water permeability due to AQP4 expression affected DWI of cell suspensions. For the 2-compartment and diffusion kurtosis imaging models, Ds was the parameter most sensitive to differences in AQP4 expression. |
| first_indexed | 2024-04-13T05:51:18Z |
| format | Article |
| id | doaj.art-634f2170efd74a78a8e8754add3ecd9e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T05:51:18Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-634f2170efd74a78a8e8754add3ecd9e2022-12-22T02:59:47ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01174e026646510.1371/journal.pone.0266465Quantitative measurement of diffusion-weighted imaging signal using expression-controlled aquaporin-4 cells: Comparative study of 2-compartment and diffusion kurtosis imaging models.Akiko ImaizumiTakayuki ObataJeff KershawYasuhiko TachibanaYoichiro AbeSayaka ShibataNobuhiro NittaIchio AokiMasato YasuiTatsuya HigashiThe purpose of this study was to compare parameter estimates for the 2-compartment and diffusion kurtosis imaging models obtained from diffusion-weighted imaging (DWI) of aquaporin-4 (AQP4) expression-controlled cells, and to look for biomarkers that indicate differences in the cell membrane water permeability. DWI was performed on AQP4-expressing and non-expressing cells and the signal was analyzed with the 2-compartment and diffusion kurtosis imaging models. For the 2-compartment model, the diffusion coefficients (Df, Ds) and volume fractions (Ff, Fs, Ff = 1-Fs) of the fast and slow compartments were estimated. For the diffusion kurtosis imaging model, estimates of the diffusion kurtosis (K) and corrected diffusion coefficient (D) were obtained. For the 2-compartment model, Ds and Fs showed clear differences between AQP4-expressing and non-expressing cells. Fs was also sensitive to cell density. There was no clear relationship with the cell type for the diffusion kurtosis imaging model parameters. Changes to cell membrane water permeability due to AQP4 expression affected DWI of cell suspensions. For the 2-compartment and diffusion kurtosis imaging models, Ds was the parameter most sensitive to differences in AQP4 expression.https://doi.org/10.1371/journal.pone.0266465 |
| spellingShingle | Akiko Imaizumi Takayuki Obata Jeff Kershaw Yasuhiko Tachibana Yoichiro Abe Sayaka Shibata Nobuhiro Nitta Ichio Aoki Masato Yasui Tatsuya Higashi Quantitative measurement of diffusion-weighted imaging signal using expression-controlled aquaporin-4 cells: Comparative study of 2-compartment and diffusion kurtosis imaging models. PLoS ONE |
| title | Quantitative measurement of diffusion-weighted imaging signal using expression-controlled aquaporin-4 cells: Comparative study of 2-compartment and diffusion kurtosis imaging models. |
| title_full | Quantitative measurement of diffusion-weighted imaging signal using expression-controlled aquaporin-4 cells: Comparative study of 2-compartment and diffusion kurtosis imaging models. |
| title_fullStr | Quantitative measurement of diffusion-weighted imaging signal using expression-controlled aquaporin-4 cells: Comparative study of 2-compartment and diffusion kurtosis imaging models. |
| title_full_unstemmed | Quantitative measurement of diffusion-weighted imaging signal using expression-controlled aquaporin-4 cells: Comparative study of 2-compartment and diffusion kurtosis imaging models. |
| title_short | Quantitative measurement of diffusion-weighted imaging signal using expression-controlled aquaporin-4 cells: Comparative study of 2-compartment and diffusion kurtosis imaging models. |
| title_sort | quantitative measurement of diffusion weighted imaging signal using expression controlled aquaporin 4 cells comparative study of 2 compartment and diffusion kurtosis imaging models |
| url | https://doi.org/10.1371/journal.pone.0266465 |
| work_keys_str_mv | AT akikoimaizumi quantitativemeasurementofdiffusionweightedimagingsignalusingexpressioncontrolledaquaporin4cellscomparativestudyof2compartmentanddiffusionkurtosisimagingmodels AT takayukiobata quantitativemeasurementofdiffusionweightedimagingsignalusingexpressioncontrolledaquaporin4cellscomparativestudyof2compartmentanddiffusionkurtosisimagingmodels AT jeffkershaw quantitativemeasurementofdiffusionweightedimagingsignalusingexpressioncontrolledaquaporin4cellscomparativestudyof2compartmentanddiffusionkurtosisimagingmodels AT yasuhikotachibana quantitativemeasurementofdiffusionweightedimagingsignalusingexpressioncontrolledaquaporin4cellscomparativestudyof2compartmentanddiffusionkurtosisimagingmodels AT yoichiroabe quantitativemeasurementofdiffusionweightedimagingsignalusingexpressioncontrolledaquaporin4cellscomparativestudyof2compartmentanddiffusionkurtosisimagingmodels AT sayakashibata quantitativemeasurementofdiffusionweightedimagingsignalusingexpressioncontrolledaquaporin4cellscomparativestudyof2compartmentanddiffusionkurtosisimagingmodels AT nobuhironitta quantitativemeasurementofdiffusionweightedimagingsignalusingexpressioncontrolledaquaporin4cellscomparativestudyof2compartmentanddiffusionkurtosisimagingmodels AT ichioaoki quantitativemeasurementofdiffusionweightedimagingsignalusingexpressioncontrolledaquaporin4cellscomparativestudyof2compartmentanddiffusionkurtosisimagingmodels AT masatoyasui quantitativemeasurementofdiffusionweightedimagingsignalusingexpressioncontrolledaquaporin4cellscomparativestudyof2compartmentanddiffusionkurtosisimagingmodels AT tatsuyahigashi quantitativemeasurementofdiffusionweightedimagingsignalusingexpressioncontrolledaquaporin4cellscomparativestudyof2compartmentanddiffusionkurtosisimagingmodels |