Chemical and Genetic Variability of Istrian <i>Foeniculum vulgare</i> Wild Populations
Wild <i>Foeniculum vulgare</i> populations from the region of Istria have been subjected to a genetic and chemical study. Headspace-gas chromatography analysis of volatile secondary metabolites and PCR-RFLP analysis of the ribosomal DNA internal transcribed spacer region has been chosen...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/17/2239 |
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| author | Mitja Križman Jernej Jakše |
| author_facet | Mitja Križman Jernej Jakše |
| author_sort | Mitja Križman |
| collection | DOAJ |
| description | Wild <i>Foeniculum vulgare</i> populations from the region of Istria have been subjected to a genetic and chemical study. Headspace-gas chromatography analysis of volatile secondary metabolites and PCR-RFLP analysis of the ribosomal DNA internal transcribed spacer region has been chosen to analyze the chemical and genetic traits of single plants, respectively. Large intrapopulation and interpopulation differences have been observed in both chemical profiles and restriction patterns of PCR products. The data from chemical and genetic analyses were pooled and used to assess allele frequencies of three putative genetic loci on individual populations. The pooled allele frequencies were used to determine interpopulation distances for phenogram reconstruction. The combined use of chemical and genetic datasets for genetic variation analysis proved to be a more comprehensive approach for such a study, compared to the use of single datasets, even using such relatively simple analytical tools. |
| first_indexed | 2024-03-10T01:23:50Z |
| format | Article |
| id | doaj.art-63519efd84064c978bcc794057c0582f |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T01:23:50Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-63519efd84064c978bcc794057c0582f2023-11-23T13:55:22ZengMDPI AGPlants2223-77472022-08-011117223910.3390/plants11172239Chemical and Genetic Variability of Istrian <i>Foeniculum vulgare</i> Wild PopulationsMitja Križman0Jernej Jakše1Laboratory of Food Chemistry, National Institute of Chemistry, Hajdrihova 19, SI-1000 Ljubljana, SloveniaAgronomy Department, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, SI-1000 Ljubljana, SloveniaWild <i>Foeniculum vulgare</i> populations from the region of Istria have been subjected to a genetic and chemical study. Headspace-gas chromatography analysis of volatile secondary metabolites and PCR-RFLP analysis of the ribosomal DNA internal transcribed spacer region has been chosen to analyze the chemical and genetic traits of single plants, respectively. Large intrapopulation and interpopulation differences have been observed in both chemical profiles and restriction patterns of PCR products. The data from chemical and genetic analyses were pooled and used to assess allele frequencies of three putative genetic loci on individual populations. The pooled allele frequencies were used to determine interpopulation distances for phenogram reconstruction. The combined use of chemical and genetic datasets for genetic variation analysis proved to be a more comprehensive approach for such a study, compared to the use of single datasets, even using such relatively simple analytical tools.https://www.mdpi.com/2223-7747/11/17/2239<i>Foeniculum vulgare</i>densitometryallele frequencyPCR-RFLPheadspace-gas chromatographyinternal transcribed spacer (ITS) |
| spellingShingle | Mitja Križman Jernej Jakše Chemical and Genetic Variability of Istrian <i>Foeniculum vulgare</i> Wild Populations Plants <i>Foeniculum vulgare</i> densitometry allele frequency PCR-RFLP headspace-gas chromatography internal transcribed spacer (ITS) |
| title | Chemical and Genetic Variability of Istrian <i>Foeniculum vulgare</i> Wild Populations |
| title_full | Chemical and Genetic Variability of Istrian <i>Foeniculum vulgare</i> Wild Populations |
| title_fullStr | Chemical and Genetic Variability of Istrian <i>Foeniculum vulgare</i> Wild Populations |
| title_full_unstemmed | Chemical and Genetic Variability of Istrian <i>Foeniculum vulgare</i> Wild Populations |
| title_short | Chemical and Genetic Variability of Istrian <i>Foeniculum vulgare</i> Wild Populations |
| title_sort | chemical and genetic variability of istrian i foeniculum vulgare i wild populations |
| topic | <i>Foeniculum vulgare</i> densitometry allele frequency PCR-RFLP headspace-gas chromatography internal transcribed spacer (ITS) |
| url | https://www.mdpi.com/2223-7747/11/17/2239 |
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