miR-933 inhibits proliferation, migration and invasion of lung cancer cell lines by regulation of KLF6 gene
Background and purpose: miRNA is supposed to be involved in the occurrence and progression of tumors. However, studies are still inadequate. This study aimed to investigate whether miR-933 can inhibit cell proliferation, migration and invasion and induce apoptosis of lung cancer ce...
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| Format: | Article |
| Language: | English |
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Editorial Office of China Oncology
2021-07-01
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| Series: | Zhongguo aizheng zazhi |
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| Online Access: | http://www.china-oncology.com/fileup/1007-3639/PDF/20210704.pdf |
| _version_ | 1811198031599501312 |
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| author | LI Haizhou , ZHANG Yanwei , XU Yingjie , YANG Men , ZHANG Lei , HAN Jingjun |
| author_facet | LI Haizhou , ZHANG Yanwei , XU Yingjie , YANG Men , ZHANG Lei , HAN Jingjun |
| author_sort | LI Haizhou , ZHANG Yanwei , XU Yingjie , YANG Men , ZHANG Lei , HAN Jingjun |
| collection | DOAJ |
| description | Background and purpose: miRNA is supposed to be involved in the occurrence and progression of tumors. However, studies are still inadequate. This study aimed to investigate whether miR-933 can inhibit cell proliferation, migration and invasion and induce apoptosis of lung cancer cell lines A549 and H460 by regulating Kruppel-like zinc finger transcription factor 6 (KLF6). Methods: Expression of miR-933 in lung cancer cell lines (A549, H460) and bronchial epithelial cells (BEAS-2B) was detected using real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR). Lung cancer cells were transfected with miR-933 mimic and mimic NC, respectively. RTFQ-PCR and Western blot were performed to detect expression of KLF6 in A549 and H460. Cell proliferation was detected by cell counting kit-8 (CCK-8) assay and EdU assay, migration and invasion were detected by transwell assay, and apoptosis was detected by Annexin Ⅴ-fluorescein isothiocyanate (FITC)/propidium iodide (PI) staining. Results: Results of RTFQ-PCR showed that the expression of KLF6 in lung cancer cell lines transfected with miR-933 mimic was obviously upregulated (P<0.05). Western blot assay displayed that expression of KLF6 protein increased in A549 and H460 transfected with miR-933 mimic separately (P<0.05). Compared with negative control, the abilities of cell proliferation, migration and invasion in both A549 and H460 cells were significantly inhibited by overexpression of miR-933 (P<0.05). Annexin Ⅴ-FITC/PI staining results showed that apoptotic rates were 48.3%±1.0% and 6.1%±0.2% respectively in A549 and H460 cells after transfection of miR-933 mimic, compared with negative control (37.6%±0.9%, 2.7%±0.01% (P<0.001). Conclusion: By regulating KLF6, miR-933 induces apoptosis, inhibits cell proliferation, reduces abilities of migration and invasion of lung cancer cells and delays the occurrence and development of lung cancer. |
| first_indexed | 2024-04-12T01:24:07Z |
| format | Article |
| id | doaj.art-63567168066148f28e9237b72964fe37 |
| institution | Directory Open Access Journal |
| issn | 1007-3639 |
| language | English |
| last_indexed | 2024-04-12T01:24:07Z |
| publishDate | 2021-07-01 |
| publisher | Editorial Office of China Oncology |
| record_format | Article |
| series | Zhongguo aizheng zazhi |
| spelling | doaj.art-63567168066148f28e9237b72964fe372022-12-22T03:53:42ZengEditorial Office of China OncologyZhongguo aizheng zazhi1007-36392021-07-0131758158810.19401/j.cnki.1007-3639.2021.07.004miR-933 inhibits proliferation, migration and invasion of lung cancer cell lines by regulation of KLF6 geneLI Haizhou , ZHANG Yanwei , XU Yingjie , YANG Men , ZHANG Lei , HAN Jingjun01. Department of Thoracic and Cardiovascular Surgery, The Eighth Affiliated Hospital of Sun Yat-sen University, Shenzhen 518000, Guangdong Province, China; 2. Department of Immune-Planning, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, Guangdong Province, China Background and purpose: miRNA is supposed to be involved in the occurrence and progression of tumors. However, studies are still inadequate. This study aimed to investigate whether miR-933 can inhibit cell proliferation, migration and invasion and induce apoptosis of lung cancer cell lines A549 and H460 by regulating Kruppel-like zinc finger transcription factor 6 (KLF6). Methods: Expression of miR-933 in lung cancer cell lines (A549, H460) and bronchial epithelial cells (BEAS-2B) was detected using real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR). Lung cancer cells were transfected with miR-933 mimic and mimic NC, respectively. RTFQ-PCR and Western blot were performed to detect expression of KLF6 in A549 and H460. Cell proliferation was detected by cell counting kit-8 (CCK-8) assay and EdU assay, migration and invasion were detected by transwell assay, and apoptosis was detected by Annexin Ⅴ-fluorescein isothiocyanate (FITC)/propidium iodide (PI) staining. Results: Results of RTFQ-PCR showed that the expression of KLF6 in lung cancer cell lines transfected with miR-933 mimic was obviously upregulated (P<0.05). Western blot assay displayed that expression of KLF6 protein increased in A549 and H460 transfected with miR-933 mimic separately (P<0.05). Compared with negative control, the abilities of cell proliferation, migration and invasion in both A549 and H460 cells were significantly inhibited by overexpression of miR-933 (P<0.05). Annexin Ⅴ-FITC/PI staining results showed that apoptotic rates were 48.3%±1.0% and 6.1%±0.2% respectively in A549 and H460 cells after transfection of miR-933 mimic, compared with negative control (37.6%±0.9%, 2.7%±0.01% (P<0.001). Conclusion: By regulating KLF6, miR-933 induces apoptosis, inhibits cell proliferation, reduces abilities of migration and invasion of lung cancer cells and delays the occurrence and development of lung cancer.http://www.china-oncology.com/fileup/1007-3639/PDF/20210704.pdfmirna| non-small cell lung cancer|cell proliferation|cell apoptosis|kruppel-like zinc finger transcription factor 6 |
| spellingShingle | LI Haizhou , ZHANG Yanwei , XU Yingjie , YANG Men , ZHANG Lei , HAN Jingjun miR-933 inhibits proliferation, migration and invasion of lung cancer cell lines by regulation of KLF6 gene Zhongguo aizheng zazhi mirna| non-small cell lung cancer|cell proliferation|cell apoptosis|kruppel-like zinc finger transcription factor 6 |
| title | miR-933 inhibits proliferation, migration and invasion of lung cancer cell lines by regulation of KLF6 gene |
| title_full | miR-933 inhibits proliferation, migration and invasion of lung cancer cell lines by regulation of KLF6 gene |
| title_fullStr | miR-933 inhibits proliferation, migration and invasion of lung cancer cell lines by regulation of KLF6 gene |
| title_full_unstemmed | miR-933 inhibits proliferation, migration and invasion of lung cancer cell lines by regulation of KLF6 gene |
| title_short | miR-933 inhibits proliferation, migration and invasion of lung cancer cell lines by regulation of KLF6 gene |
| title_sort | mir 933 inhibits proliferation migration and invasion of lung cancer cell lines by regulation of klf6 gene |
| topic | mirna| non-small cell lung cancer|cell proliferation|cell apoptosis|kruppel-like zinc finger transcription factor 6 |
| url | http://www.china-oncology.com/fileup/1007-3639/PDF/20210704.pdf |
| work_keys_str_mv | AT lihaizhouzhangyanweixuyingjieyangmenzhangleihanjingjun mir933inhibitsproliferationmigrationandinvasionoflungcancercelllinesbyregulationofklf6gene |