Use of lactulose as a prebiotic in laying hens: its effect on growth, egg production, egg quality, blood biochemistry, digestive enzymes, gene expression and intestinal morphology
Background The rising popularity of eggs as an alternative source of protein to meat has led to significant increase in egg consumption over the past decade. To meet the increasing demand for eggs, poultry farmers have used antibiotics to treat infections and, to some extent, promote growth and egg...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-10-01
|
| Series: | BMC Veterinary Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12917-023-03741-x |
| _version_ | 1827710479305277440 |
|---|---|
| author | Hassan S. Elkomy Ivan I. Koshich Sahar F. Mahmoud Magda I. Abo-Samaha |
| author_facet | Hassan S. Elkomy Ivan I. Koshich Sahar F. Mahmoud Magda I. Abo-Samaha |
| author_sort | Hassan S. Elkomy |
| collection | DOAJ |
| description | Background The rising popularity of eggs as an alternative source of protein to meat has led to significant increase in egg consumption over the past decade. To meet the increasing demand for eggs, poultry farmers have used antibiotics to treat infections and, to some extent, promote growth and egg production in raising layer. However, the emergence and global spread of antibiotic resistant bacteria has now necessitated antibiotic-free poultry farming. As alternatives to antibiotics, prebiotics are feed additives that can be used to improve the growth and laying performance of poultry which positively impacts their performance and general health. In this study we evaluated the effect of lactulose, formulated as Vetelact, on body weight, egg production, egg quality, blood biochemical parameters and expression of genes associated with reproductive performance in laying hens. Results Vetelact supplementation improved egg weight, egg production as well as egg quality. Following Vetalact supplementation, the levels of total bilirubin, total protein, globulin and phosphorus were increased, while the activities of alkaline phosphatase and lipase enzymes were increased compared to control. Vetelact at 0.10 ml/kg body weight upregulated OCX-36, OVAL, CALB1, OC-116, OCX-32 and IL8 transcripts while downregulating the transcription of Gal-10, PENK and AvBD9. At this optimal inclusion rate of Vetalect, histomorphologic analyses of intestinal tissue showed increased villi length with more goblet cell distribution and obvious mucus covering a surface, increase in the depth of intestinal crypts produce digestive enzymes, as well as more developed muscle layer that promote improved nutrient absorption. Conclusion Vetelact at a dose of 0.10 ml/ kg body weight was effective in improving productive performance of laying hens. Adding lactulose (0.10 ml/ kg body weight) to layer diet is recommended to promote growth and improve egg laying performance in antibiotics-free poultry production. |
| first_indexed | 2024-03-10T17:38:37Z |
| format | Article |
| id | doaj.art-6358750438d94346aafb6f44bda5f629 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-03-10T17:38:37Z |
| publishDate | 2023-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-6358750438d94346aafb6f44bda5f6292023-11-20T09:45:19ZengBMCBMC Veterinary Research1746-61482023-10-0119111610.1186/s12917-023-03741-xUse of lactulose as a prebiotic in laying hens: its effect on growth, egg production, egg quality, blood biochemistry, digestive enzymes, gene expression and intestinal morphologyHassan S. Elkomy0Ivan I. Koshich1Sahar F. Mahmoud2Magda I. Abo-Samaha3Poultry Breeding and Production, Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Alexandria UniversityMoscow State Academy of Veterinary Medicine and Biotechnology Named After K.I. SkryabinHistology and Cytology Department, Faculty of Veterinary Medicine, Damanhur UniversityPoultry Breeding and Production, Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Alexandria UniversityBackground The rising popularity of eggs as an alternative source of protein to meat has led to significant increase in egg consumption over the past decade. To meet the increasing demand for eggs, poultry farmers have used antibiotics to treat infections and, to some extent, promote growth and egg production in raising layer. However, the emergence and global spread of antibiotic resistant bacteria has now necessitated antibiotic-free poultry farming. As alternatives to antibiotics, prebiotics are feed additives that can be used to improve the growth and laying performance of poultry which positively impacts their performance and general health. In this study we evaluated the effect of lactulose, formulated as Vetelact, on body weight, egg production, egg quality, blood biochemical parameters and expression of genes associated with reproductive performance in laying hens. Results Vetelact supplementation improved egg weight, egg production as well as egg quality. Following Vetalact supplementation, the levels of total bilirubin, total protein, globulin and phosphorus were increased, while the activities of alkaline phosphatase and lipase enzymes were increased compared to control. Vetelact at 0.10 ml/kg body weight upregulated OCX-36, OVAL, CALB1, OC-116, OCX-32 and IL8 transcripts while downregulating the transcription of Gal-10, PENK and AvBD9. At this optimal inclusion rate of Vetalect, histomorphologic analyses of intestinal tissue showed increased villi length with more goblet cell distribution and obvious mucus covering a surface, increase in the depth of intestinal crypts produce digestive enzymes, as well as more developed muscle layer that promote improved nutrient absorption. Conclusion Vetelact at a dose of 0.10 ml/ kg body weight was effective in improving productive performance of laying hens. Adding lactulose (0.10 ml/ kg body weight) to layer diet is recommended to promote growth and improve egg laying performance in antibiotics-free poultry production.https://doi.org/10.1186/s12917-023-03741-xVetelactLactuloseLayerOCX-36OVALOC-116 |
| spellingShingle | Hassan S. Elkomy Ivan I. Koshich Sahar F. Mahmoud Magda I. Abo-Samaha Use of lactulose as a prebiotic in laying hens: its effect on growth, egg production, egg quality, blood biochemistry, digestive enzymes, gene expression and intestinal morphology BMC Veterinary Research Vetelact Lactulose Layer OCX-36 OVAL OC-116 |
| title | Use of lactulose as a prebiotic in laying hens: its effect on growth, egg production, egg quality, blood biochemistry, digestive enzymes, gene expression and intestinal morphology |
| title_full | Use of lactulose as a prebiotic in laying hens: its effect on growth, egg production, egg quality, blood biochemistry, digestive enzymes, gene expression and intestinal morphology |
| title_fullStr | Use of lactulose as a prebiotic in laying hens: its effect on growth, egg production, egg quality, blood biochemistry, digestive enzymes, gene expression and intestinal morphology |
| title_full_unstemmed | Use of lactulose as a prebiotic in laying hens: its effect on growth, egg production, egg quality, blood biochemistry, digestive enzymes, gene expression and intestinal morphology |
| title_short | Use of lactulose as a prebiotic in laying hens: its effect on growth, egg production, egg quality, blood biochemistry, digestive enzymes, gene expression and intestinal morphology |
| title_sort | use of lactulose as a prebiotic in laying hens its effect on growth egg production egg quality blood biochemistry digestive enzymes gene expression and intestinal morphology |
| topic | Vetelact Lactulose Layer OCX-36 OVAL OC-116 |
| url | https://doi.org/10.1186/s12917-023-03741-x |
| work_keys_str_mv | AT hassanselkomy useoflactuloseasaprebioticinlayinghensitseffectongrowtheggproductioneggqualitybloodbiochemistrydigestiveenzymesgeneexpressionandintestinalmorphology AT ivanikoshich useoflactuloseasaprebioticinlayinghensitseffectongrowtheggproductioneggqualitybloodbiochemistrydigestiveenzymesgeneexpressionandintestinalmorphology AT saharfmahmoud useoflactuloseasaprebioticinlayinghensitseffectongrowtheggproductioneggqualitybloodbiochemistrydigestiveenzymesgeneexpressionandintestinalmorphology AT magdaiabosamaha useoflactuloseasaprebioticinlayinghensitseffectongrowtheggproductioneggqualitybloodbiochemistrydigestiveenzymesgeneexpressionandintestinalmorphology |