Electrochemical Detection of Insulin in Blood serum using Ppy/GF Nanocomposite Modified Pencil Graphite Electrode
In this study, pencil graphite electrode was modified using conductive polypyrrole (Ppy) and grapheme (GF) nanocomposite for electrochemical determination of insulin. Electrochemical behavior of insulin on PGE was investigated using cyclic voltammetric (CV) and differential pulse voltammetric (DPV)...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Iranian Society of Nanomedicine
2018-12-01
|
| Series: | Nanomedicine Research Journal |
| Subjects: | |
| Online Access: | http://www.nanomedicine-rj.com/article_33845_278c37ea36b7ad3aab78fa37aae25f7f.pdf |
| _version_ | 1818361704052948992 |
|---|---|
| author | Saeideh Ebrahimiasl Elham Fathi Mansor Ahmad |
| author_facet | Saeideh Ebrahimiasl Elham Fathi Mansor Ahmad |
| author_sort | Saeideh Ebrahimiasl |
| collection | DOAJ |
| description | In this study, pencil graphite electrode was modified using conductive polypyrrole (Ppy) and grapheme (GF) nanocomposite for electrochemical determination of insulin. Electrochemical behavior of insulin on PGE was investigated using cyclic voltammetric (CV) and differential pulse voltammetric (DPV) and chronoaprometry (CA) methods. Several effective parameters including pH, concentration, and scan rate for electrochemical modification of electrode were investigated and optimal conditions were proposed. Kinetics of the oxidation reaction and diffusion coefficient of the sensor was studied. The performed steps allow the measurement of insulin with a linear repeatability curve and appropriate accuracy at a range of 0.225 to 1.235 μM. The limit of detection was obtained at 8.65 nM for insulin. The amount of electron transfer coefficient between modified electrode and insulin was obtained to 0.5 with 0.84~1 number of electrons exchanged during oxidation of insulin. The application of proposed sensor for analyzing insulin in a human blood serum was investigated. |
| first_indexed | 2024-12-13T21:20:55Z |
| format | Article |
| id | doaj.art-635bfcaec4be4fd1857af8cc2315a2e4 |
| institution | Directory Open Access Journal |
| issn | 2476-3489 2476-7123 |
| language | English |
| last_indexed | 2024-12-13T21:20:55Z |
| publishDate | 2018-12-01 |
| publisher | Iranian Society of Nanomedicine |
| record_format | Article |
| series | Nanomedicine Research Journal |
| spelling | doaj.art-635bfcaec4be4fd1857af8cc2315a2e42022-12-21T23:31:07ZengIranian Society of NanomedicineNanomedicine Research Journal2476-34892476-71232018-12-013421922810.22034/nmrj.2018.04.00633845Electrochemical Detection of Insulin in Blood serum using Ppy/GF Nanocomposite Modified Pencil Graphite ElectrodeSaeideh Ebrahimiasl0Elham Fathi1Mansor Ahmad2Department of Chemistry, Ahar Branch, Islamic Azad University, Ahar, Iran || Industrial Nanotechnology Research Center, Islamic Azad University, Tabriz, IranDepartment of Chemical Engineering, Ahar Branch, Islamic Azad University, Ahar, IranDepartment of Chemistry, University Putra Malaysia, 43400, Serdang, MalaysiaIn this study, pencil graphite electrode was modified using conductive polypyrrole (Ppy) and grapheme (GF) nanocomposite for electrochemical determination of insulin. Electrochemical behavior of insulin on PGE was investigated using cyclic voltammetric (CV) and differential pulse voltammetric (DPV) and chronoaprometry (CA) methods. Several effective parameters including pH, concentration, and scan rate for electrochemical modification of electrode were investigated and optimal conditions were proposed. Kinetics of the oxidation reaction and diffusion coefficient of the sensor was studied. The performed steps allow the measurement of insulin with a linear repeatability curve and appropriate accuracy at a range of 0.225 to 1.235 μM. The limit of detection was obtained at 8.65 nM for insulin. The amount of electron transfer coefficient between modified electrode and insulin was obtained to 0.5 with 0.84~1 number of electrons exchanged during oxidation of insulin. The application of proposed sensor for analyzing insulin in a human blood serum was investigated.http://www.nanomedicine-rj.com/article_33845_278c37ea36b7ad3aab78fa37aae25f7f.pdfinsulinblood serumnanosensorpencil graphite electrodesgraphene |
| spellingShingle | Saeideh Ebrahimiasl Elham Fathi Mansor Ahmad Electrochemical Detection of Insulin in Blood serum using Ppy/GF Nanocomposite Modified Pencil Graphite Electrode Nanomedicine Research Journal insulin blood serum nanosensor pencil graphite electrodes graphene |
| title | Electrochemical Detection of Insulin in Blood serum using Ppy/GF Nanocomposite Modified Pencil Graphite Electrode |
| title_full | Electrochemical Detection of Insulin in Blood serum using Ppy/GF Nanocomposite Modified Pencil Graphite Electrode |
| title_fullStr | Electrochemical Detection of Insulin in Blood serum using Ppy/GF Nanocomposite Modified Pencil Graphite Electrode |
| title_full_unstemmed | Electrochemical Detection of Insulin in Blood serum using Ppy/GF Nanocomposite Modified Pencil Graphite Electrode |
| title_short | Electrochemical Detection of Insulin in Blood serum using Ppy/GF Nanocomposite Modified Pencil Graphite Electrode |
| title_sort | electrochemical detection of insulin in blood serum using ppy gf nanocomposite modified pencil graphite electrode |
| topic | insulin blood serum nanosensor pencil graphite electrodes graphene |
| url | http://www.nanomedicine-rj.com/article_33845_278c37ea36b7ad3aab78fa37aae25f7f.pdf |
| work_keys_str_mv | AT saeidehebrahimiasl electrochemicaldetectionofinsulininbloodserumusingppygfnanocompositemodifiedpencilgraphiteelectrode AT elhamfathi electrochemicaldetectionofinsulininbloodserumusingppygfnanocompositemodifiedpencilgraphiteelectrode AT mansorahmad electrochemicaldetectionofinsulininbloodserumusingppygfnanocompositemodifiedpencilgraphiteelectrode |