De Novo Assembly and Characterization of the Transcriptome of Grasshopper Shirakiacris shirakii
Background: The grasshopper Shirakiacris shirakii is an important agricultural pest and feeds mainly on gramineous plants, thereby causing economic damage to a wide range of crops. However, genomic information on this species is extremely limited thus far, and transcriptome data relevant to insectic...
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MDPI AG
2016-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | http://www.mdpi.com/1422-0067/17/7/1110 |
| _version_ | 1811295656525955072 |
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| author | Zhongying Qiu Fei Liu Huimeng Lu Hao Yuan Qin Zhang Yuan Huang |
| author_facet | Zhongying Qiu Fei Liu Huimeng Lu Hao Yuan Qin Zhang Yuan Huang |
| author_sort | Zhongying Qiu |
| collection | DOAJ |
| description | Background: The grasshopper Shirakiacris shirakii is an important agricultural pest and feeds mainly on gramineous plants, thereby causing economic damage to a wide range of crops. However, genomic information on this species is extremely limited thus far, and transcriptome data relevant to insecticide resistance and pest control are also not available. Methods: The transcriptome of S. shirakii was sequenced using the Illumina HiSeq platform, and we de novo assembled the transcriptome. Results: Its sequencing produced a total of 105,408,878 clean reads, and the de novo assembly revealed 74,657 unigenes with an average length of 680 bp and N50 of 1057 bp. A total of 28,173 unigenes were annotated for the NCBI non-redundant protein sequences (Nr), NCBI non-redundant nucleotide sequences (Nt), a manually-annotated and reviewed protein sequence database (Swiss-Prot), Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Based on the Nr annotation results, we manually identified 79 unigenes encoding cytochrome P450 monooxygenases (P450s), 36 unigenes encoding carboxylesterases (CarEs) and 36 unigenes encoding glutathione S-transferases (GSTs) in S. shirakii. Core RNAi components relevant to miroRNA, siRNA and piRNA pathways, including Pasha, Loquacious, Argonaute-1, Argonaute-2, Argonaute-3, Zucchini, Aubergine, enhanced RNAi-1 and Piwi, were expressed in S. shirakii. We also identified five unigenes that were homologous to the Sid-1 gene. In addition, the analysis of differential gene expressions revealed that a total of 19,764 unigenes were up-regulated and 4185 unigenes were down-regulated in larvae. In total, we predicted 7504 simple sequence repeats (SSRs) from 74,657 unigenes. Conclusions: The comprehensive de novo transcriptomic data of S. shirakii will offer a series of valuable molecular resources for better studying insecticide resistance, RNAi and molecular marker discovery in the transcriptome. |
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| spelling | doaj.art-635e04ddba4a4f79b27adb4b5fbb58212022-12-22T03:00:16ZengMDPI AGInternational Journal of Molecular Sciences1422-00672016-07-01177111010.3390/ijms17071110ijms17071110De Novo Assembly and Characterization of the Transcriptome of Grasshopper Shirakiacris shirakiiZhongying Qiu0Fei Liu1Huimeng Lu2Hao Yuan3Qin Zhang4Yuan Huang5College of Life Sciences, Shaanxi Normal University, Xi’an 710062, ChinaCollege of Life Sciences, Shaanxi Normal University, Xi’an 710062, ChinaKey Laboratory for Space Bioscience & Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an 710072, ChinaCollege of Life Sciences, Shaanxi Normal University, Xi’an 710062, ChinaCollege of Life Sciences, Shaanxi Normal University, Xi’an 710062, ChinaCollege of Life Sciences, Shaanxi Normal University, Xi’an 710062, ChinaBackground: The grasshopper Shirakiacris shirakii is an important agricultural pest and feeds mainly on gramineous plants, thereby causing economic damage to a wide range of crops. However, genomic information on this species is extremely limited thus far, and transcriptome data relevant to insecticide resistance and pest control are also not available. Methods: The transcriptome of S. shirakii was sequenced using the Illumina HiSeq platform, and we de novo assembled the transcriptome. Results: Its sequencing produced a total of 105,408,878 clean reads, and the de novo assembly revealed 74,657 unigenes with an average length of 680 bp and N50 of 1057 bp. A total of 28,173 unigenes were annotated for the NCBI non-redundant protein sequences (Nr), NCBI non-redundant nucleotide sequences (Nt), a manually-annotated and reviewed protein sequence database (Swiss-Prot), Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Based on the Nr annotation results, we manually identified 79 unigenes encoding cytochrome P450 monooxygenases (P450s), 36 unigenes encoding carboxylesterases (CarEs) and 36 unigenes encoding glutathione S-transferases (GSTs) in S. shirakii. Core RNAi components relevant to miroRNA, siRNA and piRNA pathways, including Pasha, Loquacious, Argonaute-1, Argonaute-2, Argonaute-3, Zucchini, Aubergine, enhanced RNAi-1 and Piwi, were expressed in S. shirakii. We also identified five unigenes that were homologous to the Sid-1 gene. In addition, the analysis of differential gene expressions revealed that a total of 19,764 unigenes were up-regulated and 4185 unigenes were down-regulated in larvae. In total, we predicted 7504 simple sequence repeats (SSRs) from 74,657 unigenes. Conclusions: The comprehensive de novo transcriptomic data of S. shirakii will offer a series of valuable molecular resources for better studying insecticide resistance, RNAi and molecular marker discovery in the transcriptome.http://www.mdpi.com/1422-0067/17/7/1110Shirakiacris shirakiitranscriptomeinsecticide resistanceRNAidifferentially-expressed unigenes (DEUs) |
| spellingShingle | Zhongying Qiu Fei Liu Huimeng Lu Hao Yuan Qin Zhang Yuan Huang De Novo Assembly and Characterization of the Transcriptome of Grasshopper Shirakiacris shirakii International Journal of Molecular Sciences Shirakiacris shirakii transcriptome insecticide resistance RNAi differentially-expressed unigenes (DEUs) |
| title | De Novo Assembly and Characterization of the Transcriptome of Grasshopper Shirakiacris shirakii |
| title_full | De Novo Assembly and Characterization of the Transcriptome of Grasshopper Shirakiacris shirakii |
| title_fullStr | De Novo Assembly and Characterization of the Transcriptome of Grasshopper Shirakiacris shirakii |
| title_full_unstemmed | De Novo Assembly and Characterization of the Transcriptome of Grasshopper Shirakiacris shirakii |
| title_short | De Novo Assembly and Characterization of the Transcriptome of Grasshopper Shirakiacris shirakii |
| title_sort | de novo assembly and characterization of the transcriptome of grasshopper shirakiacris shirakii |
| topic | Shirakiacris shirakii transcriptome insecticide resistance RNAi differentially-expressed unigenes (DEUs) |
| url | http://www.mdpi.com/1422-0067/17/7/1110 |
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