Truncated isoforms of GPSM2 containing the GoLoco motif region promote CD4+ T-cell migration in SLE
Objective SLE is an autoimmune disease with a complex pathogenesis. T-cell infiltration into organs contributes to inflammation and organ damage in SLE. Recently, G-protein signalling modulator 2 (GPSM2) has been shown to be implicated in T-cell migration.Methods We analysed the expression levels of...
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| Format: | Article |
| Language: | English |
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BMJ Publishing Group
2022-07-01
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| Series: | Lupus Science and Medicine |
| Online Access: | https://lupus.bmj.com/content/9/1/e000742.full |
| _version_ | 1827901080991694848 |
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| author | Anja Meyer Joanna Schiller David M Kofler Ruth L Esser Carolin Brück Jan Thiele Viktoria Golumba-Nagy Eva Steinbach-Knödgen Shuaifeng Yan Carola tho Pesch David Stahl |
| author_facet | Anja Meyer Joanna Schiller David M Kofler Ruth L Esser Carolin Brück Jan Thiele Viktoria Golumba-Nagy Eva Steinbach-Knödgen Shuaifeng Yan Carola tho Pesch David Stahl |
| author_sort | Anja Meyer |
| collection | DOAJ |
| description | Objective SLE is an autoimmune disease with a complex pathogenesis. T-cell infiltration into organs contributes to inflammation and organ damage in SLE. Recently, G-protein signalling modulator 2 (GPSM2) has been shown to be implicated in T-cell migration.Methods We analysed the expression levels of GPSM2 and of a truncated isoform of GPSM2 containing the GoLoco motif region in CD4+ T cells from patients with SLE and from healthy individuals by western blot. In a next step, we studied the role of the truncated GPSM2 isoform using a CD4+ T-cell migration assay.Results Our experiments revealed comparable levels of GPSM2 in CD4+ T cells from patients with SLE and healthy controls. In contrast, the truncated 35 kDa isoform of GPSM2 was significantly more highly expressed in CD4+ T cells from patients with SLE as compared with healthy subjects. Antibody-mediated blockade of the 35 kDa GPSM2 isoform reduced the in vitro capacity of CD4+ T cells to migrate towards the chemokine CCL20.Conclusions A truncated GPSM2 isoform containing the GoLoco motif region is upregulated in CD4+ T cells from patients with SLE and promotes CD4+ T-cell migration. Targeting this isoform with specific antibodies might be a promising approach to reduce CD4+ T-cell infiltration into inflamed tissues and to prevent organ damage in SLE. |
| first_indexed | 2024-03-12T23:40:37Z |
| format | Article |
| id | doaj.art-6360ca39f1754987b5eaad4c50abe7cf |
| institution | Directory Open Access Journal |
| issn | 2053-8790 |
| language | English |
| last_indexed | 2024-03-12T23:40:37Z |
| publishDate | 2022-07-01 |
| publisher | BMJ Publishing Group |
| record_format | Article |
| series | Lupus Science and Medicine |
| spelling | doaj.art-6360ca39f1754987b5eaad4c50abe7cf2023-07-14T16:00:07ZengBMJ Publishing GroupLupus Science and Medicine2053-87902022-07-019110.1136/lupus-2022-000742Truncated isoforms of GPSM2 containing the GoLoco motif region promote CD4+ T-cell migration in SLEAnja Meyer0Joanna Schiller1David M Kofler2Ruth L Esser3Carolin Brück4Jan Thiele5Viktoria Golumba-Nagy6Eva Steinbach-Knödgen7Shuaifeng Yan8Carola tho Pesch9David Stahl102 Department of Medicine, University of Illinois Chicago, Chicago, Illinois, USADepartment I of Internal Medicine, University Hospital Cologne, Cologne, GermanyDepartment I of Internal Medicine, University Hospital Cologne, Cologne, GermanyDepartment I of Internal Medicine, University Hospital Cologne, Cologne, GermanyDepartment I of Internal Medicine, University Hospital Cologne, Cologne, GermanyDepartment I of Internal Medicine, University Hospital Cologne, Cologne, GermanyDepartment I of Internal Medicine, University Hospital Cologne, Cologne, GermanyDepartment I of Internal Medicine, University Hospital Cologne, Cologne, GermanyDepartment I of Internal Medicine, University Hospital Cologne, Cologne, GermanyDepartment I of Internal Medicine, University Hospital Cologne, Cologne, GermanyDepartment I of Internal Medicine, University Hospital Cologne, Cologne, GermanyObjective SLE is an autoimmune disease with a complex pathogenesis. T-cell infiltration into organs contributes to inflammation and organ damage in SLE. Recently, G-protein signalling modulator 2 (GPSM2) has been shown to be implicated in T-cell migration.Methods We analysed the expression levels of GPSM2 and of a truncated isoform of GPSM2 containing the GoLoco motif region in CD4+ T cells from patients with SLE and from healthy individuals by western blot. In a next step, we studied the role of the truncated GPSM2 isoform using a CD4+ T-cell migration assay.Results Our experiments revealed comparable levels of GPSM2 in CD4+ T cells from patients with SLE and healthy controls. In contrast, the truncated 35 kDa isoform of GPSM2 was significantly more highly expressed in CD4+ T cells from patients with SLE as compared with healthy subjects. Antibody-mediated blockade of the 35 kDa GPSM2 isoform reduced the in vitro capacity of CD4+ T cells to migrate towards the chemokine CCL20.Conclusions A truncated GPSM2 isoform containing the GoLoco motif region is upregulated in CD4+ T cells from patients with SLE and promotes CD4+ T-cell migration. Targeting this isoform with specific antibodies might be a promising approach to reduce CD4+ T-cell infiltration into inflamed tissues and to prevent organ damage in SLE.https://lupus.bmj.com/content/9/1/e000742.full |
| spellingShingle | Anja Meyer Joanna Schiller David M Kofler Ruth L Esser Carolin Brück Jan Thiele Viktoria Golumba-Nagy Eva Steinbach-Knödgen Shuaifeng Yan Carola tho Pesch David Stahl Truncated isoforms of GPSM2 containing the GoLoco motif region promote CD4+ T-cell migration in SLE Lupus Science and Medicine |
| title | Truncated isoforms of GPSM2 containing the GoLoco motif region promote CD4+ T-cell migration in SLE |
| title_full | Truncated isoforms of GPSM2 containing the GoLoco motif region promote CD4+ T-cell migration in SLE |
| title_fullStr | Truncated isoforms of GPSM2 containing the GoLoco motif region promote CD4+ T-cell migration in SLE |
| title_full_unstemmed | Truncated isoforms of GPSM2 containing the GoLoco motif region promote CD4+ T-cell migration in SLE |
| title_short | Truncated isoforms of GPSM2 containing the GoLoco motif region promote CD4+ T-cell migration in SLE |
| title_sort | truncated isoforms of gpsm2 containing the goloco motif region promote cd4 t cell migration in sle |
| url | https://lupus.bmj.com/content/9/1/e000742.full |
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