Heterologous Expression of Dehydration-Inducible <i>MfbHLH145</i> of <i>Myrothamnus flabellifoli</i> Enhanced Drought and Salt Tolerance in Arabidopsis
<i>Myrothamnus flabellifolia</i> is the only woody resurrection plant found in the world. It has a strong tolerance to drought and can survive long-term exposure to desiccated environments. However, few genes related to its drought tolerance have been functionally characterized and the m...
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MDPI AG
2022-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/10/5546 |
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| author | Zhuo Huang Si-Han Jin Li Yang Li Song Yuan-Hong Wang Lin-Li Jian Cai-Zhong Jiang |
| author_facet | Zhuo Huang Si-Han Jin Li Yang Li Song Yuan-Hong Wang Lin-Li Jian Cai-Zhong Jiang |
| author_sort | Zhuo Huang |
| collection | DOAJ |
| description | <i>Myrothamnus flabellifolia</i> is the only woody resurrection plant found in the world. It has a strong tolerance to drought and can survive long-term exposure to desiccated environments. However, few genes related to its drought tolerance have been functionally characterized and the molecular mechanisms underlying the stress tolerance of <i>M. flabellifolia</i> are largely unknown. In this study, we isolated a dehydration-inducible bHLH transcription factor gene <i>MfbHLH145</i> from <i>M. flabellifolia</i>. Heterologous expression of <i>MfbHLH145</i> enhanced the drought and salt tolerance of Arabidopsis. It can not only promote root system development under short-term stresses, but also improve growth performance under long-term treatments. Further investigation showed that <i>MfbHLH145</i> contributes to enhanced leaf water retention capacity through the promotion of stomatal closure, increased osmolyte accumulation, and decreased stress-induced oxidative damage through an increase in antioxidant enzyme activities. These results suggest that <i>MfbHLH145</i> may be involved in the positive regulation of stress responses in <i>M. flabellifolia</i>. This study provides insight into the molecular mechanism underlying the survival of <i>M. flabellifolia</i> in extreme dehydration conditions. |
| first_indexed | 2024-03-10T03:44:25Z |
| format | Article |
| id | doaj.art-636289deadce4e9886a899160cd9c1dd |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T03:44:25Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-636289deadce4e9886a899160cd9c1dd2023-11-23T11:24:29ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-05-012310554610.3390/ijms23105546Heterologous Expression of Dehydration-Inducible <i>MfbHLH145</i> of <i>Myrothamnus flabellifoli</i> Enhanced Drought and Salt Tolerance in ArabidopsisZhuo Huang0Si-Han Jin1Li Yang2Li Song3Yuan-Hong Wang4Lin-Li Jian5Cai-Zhong Jiang6College of Landscape Architecture, Sichuan Agricultural University, Wenjiang 611130, ChinaCollege of Landscape Architecture, Sichuan Agricultural University, Wenjiang 611130, ChinaCollege of Landscape Architecture, Sichuan Agricultural University, Wenjiang 611130, ChinaCollege of Landscape Architecture, Sichuan Agricultural University, Wenjiang 611130, ChinaCollege of Landscape Architecture, Sichuan Agricultural University, Wenjiang 611130, ChinaCollege of Landscape Architecture, Sichuan Agricultural University, Wenjiang 611130, ChinaDepartment of Plant Sciences, University of California Davis, Davis, CA 95616, USA<i>Myrothamnus flabellifolia</i> is the only woody resurrection plant found in the world. It has a strong tolerance to drought and can survive long-term exposure to desiccated environments. However, few genes related to its drought tolerance have been functionally characterized and the molecular mechanisms underlying the stress tolerance of <i>M. flabellifolia</i> are largely unknown. In this study, we isolated a dehydration-inducible bHLH transcription factor gene <i>MfbHLH145</i> from <i>M. flabellifolia</i>. Heterologous expression of <i>MfbHLH145</i> enhanced the drought and salt tolerance of Arabidopsis. It can not only promote root system development under short-term stresses, but also improve growth performance under long-term treatments. Further investigation showed that <i>MfbHLH145</i> contributes to enhanced leaf water retention capacity through the promotion of stomatal closure, increased osmolyte accumulation, and decreased stress-induced oxidative damage through an increase in antioxidant enzyme activities. These results suggest that <i>MfbHLH145</i> may be involved in the positive regulation of stress responses in <i>M. flabellifolia</i>. This study provides insight into the molecular mechanism underlying the survival of <i>M. flabellifolia</i> in extreme dehydration conditions.https://www.mdpi.com/1422-0067/23/10/5546<i>Myrothamnus flabellifolia</i>resurrection plantdrought toleranceArabidopsisbasic helix–loop–helix (bHLH) |
| spellingShingle | Zhuo Huang Si-Han Jin Li Yang Li Song Yuan-Hong Wang Lin-Li Jian Cai-Zhong Jiang Heterologous Expression of Dehydration-Inducible <i>MfbHLH145</i> of <i>Myrothamnus flabellifoli</i> Enhanced Drought and Salt Tolerance in Arabidopsis International Journal of Molecular Sciences <i>Myrothamnus flabellifolia</i> resurrection plant drought tolerance Arabidopsis basic helix–loop–helix (bHLH) |
| title | Heterologous Expression of Dehydration-Inducible <i>MfbHLH145</i> of <i>Myrothamnus flabellifoli</i> Enhanced Drought and Salt Tolerance in Arabidopsis |
| title_full | Heterologous Expression of Dehydration-Inducible <i>MfbHLH145</i> of <i>Myrothamnus flabellifoli</i> Enhanced Drought and Salt Tolerance in Arabidopsis |
| title_fullStr | Heterologous Expression of Dehydration-Inducible <i>MfbHLH145</i> of <i>Myrothamnus flabellifoli</i> Enhanced Drought and Salt Tolerance in Arabidopsis |
| title_full_unstemmed | Heterologous Expression of Dehydration-Inducible <i>MfbHLH145</i> of <i>Myrothamnus flabellifoli</i> Enhanced Drought and Salt Tolerance in Arabidopsis |
| title_short | Heterologous Expression of Dehydration-Inducible <i>MfbHLH145</i> of <i>Myrothamnus flabellifoli</i> Enhanced Drought and Salt Tolerance in Arabidopsis |
| title_sort | heterologous expression of dehydration inducible i mfbhlh145 i of i myrothamnus flabellifoli i enhanced drought and salt tolerance in arabidopsis |
| topic | <i>Myrothamnus flabellifolia</i> resurrection plant drought tolerance Arabidopsis basic helix–loop–helix (bHLH) |
| url | https://www.mdpi.com/1422-0067/23/10/5546 |
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