Transcriptome Profile of <i>Fusarium graminearum</i> Treated by Putrescine
<i>Fusarium graminearum</i> (<i>F. graminearum</i>) is the main pathogen of Fusarium head blight (FHB) in wheat, barley, and corn. Deoxynivalenol (DON), produced by <i>F. graminearum</i>, is the most prevalent toxin associated with FHB. The wheat defense compound...
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MDPI AG
2022-12-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/9/1/60 |
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| author | Lina Zhang Xishi Zhou Pengfeng Li Yiwei Wang Qianyong Hu Yuping Shang Yunshen Chen Xiying Zhu Hongjie Feng Cuijun Zhang |
| author_facet | Lina Zhang Xishi Zhou Pengfeng Li Yiwei Wang Qianyong Hu Yuping Shang Yunshen Chen Xiying Zhu Hongjie Feng Cuijun Zhang |
| author_sort | Lina Zhang |
| collection | DOAJ |
| description | <i>Fusarium graminearum</i> (<i>F. graminearum</i>) is the main pathogen of Fusarium head blight (FHB) in wheat, barley, and corn. Deoxynivalenol (DON), produced by <i>F. graminearum</i>, is the most prevalent toxin associated with FHB. The wheat defense compound putrescine can promote DON production during <i>F. graminearum</i> infection. However, the underlying mechanisms of putrescine-induced DON synthesis are not well-studied. To investigate the effect of putrescine on the global transcriptional regulation of <i>F. graminearum</i>, we treated <i>F. graminearum</i> with putrescine and performed RNA deep sequencing. We found that putrescine can largely affect the transcriptome of <i>F. graminearum</i>. Gene ontology (GO) and KEGG enrichment analysis revealed that having a large amount of DEGs was associated with ribosome biogenesis, carboxylic acid metabolism, glycolysis/gluconeogenesis, and amino acid metabolism pathways. Co-expression analysis showed that 327 genes had similar expression patterns to <i>FgTRI</i> genes and were assigned to the same module. In addition, three transcription factor genes were identified as hub genes in this module, indicating that they may play important roles in DON synthesis. These results provide important clues for further analysis of the molecular mechanisms of putrescine-induced DON synthesis and will facilitate the study of the pathogenic mechanisms of FHB. |
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| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T12:04:41Z |
| publishDate | 2022-12-01 |
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| series | Journal of Fungi |
| spelling | doaj.art-63640a38a1d04b83a92c3340f8e518392023-11-30T22:59:46ZengMDPI AGJournal of Fungi2309-608X2022-12-01916010.3390/jof9010060Transcriptome Profile of <i>Fusarium graminearum</i> Treated by PutrescineLina Zhang0Xishi Zhou1Pengfeng Li2Yiwei Wang3Qianyong Hu4Yuping Shang5Yunshen Chen6Xiying Zhu7Hongjie Feng8Cuijun Zhang9Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaShenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, ChinaShenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, ChinaShenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, ChinaShenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, ChinaShenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, ChinaShenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, ChinaShenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, ChinaZhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaShenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China<i>Fusarium graminearum</i> (<i>F. graminearum</i>) is the main pathogen of Fusarium head blight (FHB) in wheat, barley, and corn. Deoxynivalenol (DON), produced by <i>F. graminearum</i>, is the most prevalent toxin associated with FHB. The wheat defense compound putrescine can promote DON production during <i>F. graminearum</i> infection. However, the underlying mechanisms of putrescine-induced DON synthesis are not well-studied. To investigate the effect of putrescine on the global transcriptional regulation of <i>F. graminearum</i>, we treated <i>F. graminearum</i> with putrescine and performed RNA deep sequencing. We found that putrescine can largely affect the transcriptome of <i>F. graminearum</i>. Gene ontology (GO) and KEGG enrichment analysis revealed that having a large amount of DEGs was associated with ribosome biogenesis, carboxylic acid metabolism, glycolysis/gluconeogenesis, and amino acid metabolism pathways. Co-expression analysis showed that 327 genes had similar expression patterns to <i>FgTRI</i> genes and were assigned to the same module. In addition, three transcription factor genes were identified as hub genes in this module, indicating that they may play important roles in DON synthesis. These results provide important clues for further analysis of the molecular mechanisms of putrescine-induced DON synthesis and will facilitate the study of the pathogenic mechanisms of FHB.https://www.mdpi.com/2309-608X/9/1/60deoxynivalenol synthesis<i>Fusarium graminearum</i>putrescinetranscriptome |
| spellingShingle | Lina Zhang Xishi Zhou Pengfeng Li Yiwei Wang Qianyong Hu Yuping Shang Yunshen Chen Xiying Zhu Hongjie Feng Cuijun Zhang Transcriptome Profile of <i>Fusarium graminearum</i> Treated by Putrescine Journal of Fungi deoxynivalenol synthesis <i>Fusarium graminearum</i> putrescine transcriptome |
| title | Transcriptome Profile of <i>Fusarium graminearum</i> Treated by Putrescine |
| title_full | Transcriptome Profile of <i>Fusarium graminearum</i> Treated by Putrescine |
| title_fullStr | Transcriptome Profile of <i>Fusarium graminearum</i> Treated by Putrescine |
| title_full_unstemmed | Transcriptome Profile of <i>Fusarium graminearum</i> Treated by Putrescine |
| title_short | Transcriptome Profile of <i>Fusarium graminearum</i> Treated by Putrescine |
| title_sort | transcriptome profile of i fusarium graminearum i treated by putrescine |
| topic | deoxynivalenol synthesis <i>Fusarium graminearum</i> putrescine transcriptome |
| url | https://www.mdpi.com/2309-608X/9/1/60 |
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