Transcriptome Analyses of Barley Roots Inoculated with Novel <i>Paenibacillus</i> sp. and <i>Erwinia gerundensis</i> Strains Reveal Beneficial Early-Stage Plant–Bacteria Interactions
Plant growth-promoting bacteria can improve host plant traits including nutrient uptake and metabolism and tolerance to biotic and abiotic stresses. Understanding the molecular basis of plant–bacteria interactions using dual RNA-seq analyses provides key knowledge of both host and bacteria simultane...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-08-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/9/1802 |
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| author | Tongda Li Ross Mann Jatinder Kaur German Spangenberg Timothy Sawbridge |
| author_facet | Tongda Li Ross Mann Jatinder Kaur German Spangenberg Timothy Sawbridge |
| author_sort | Tongda Li |
| collection | DOAJ |
| description | Plant growth-promoting bacteria can improve host plant traits including nutrient uptake and metabolism and tolerance to biotic and abiotic stresses. Understanding the molecular basis of plant–bacteria interactions using dual RNA-seq analyses provides key knowledge of both host and bacteria simultaneously, leading to future enhancements of beneficial interactions. In this study, dual RNA-seq analyses were performed to provide insights into the early-stage interactions between barley seedlings and three novel bacterial strains (two <i>Paenibacillus</i> sp. strains and one <i>Erwinia gerundensis</i> strain) isolated from the perennial ryegrass seed microbiome. Differentially expressed bacterial and barley genes/transcripts involved in plant–bacteria interactions were identified, with varying species- and strain-specific responses. Overall, transcriptome profiles suggested that all three strains improved stress response, signal transduction, and nutrient uptake and metabolism of barley seedlings. Results also suggested potential improvements in seedling root growth via repressing ethylene biosynthesis in roots. Bacterial secondary metabolite gene clusters producing compounds that are potentially associated with interactions with the barley endophytic microbiome and associated with stress tolerance of plants under nutrient limiting conditions were also identified. The results of this study provided the molecular basis of plant growth-promoting activities of three novel bacterial strains in barley, laid a solid foundation for the future development of these three bacterial strains as biofertilisers, and identified key differences between bacterial strains of the same species in their responses to plants. |
| first_indexed | 2024-03-10T07:17:22Z |
| format | Article |
| id | doaj.art-636477553192478dbd5f4b3d1377ccdd |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T07:17:22Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-636477553192478dbd5f4b3d1377ccdd2023-11-22T14:51:56ZengMDPI AGPlants2223-77472021-08-01109180210.3390/plants10091802Transcriptome Analyses of Barley Roots Inoculated with Novel <i>Paenibacillus</i> sp. and <i>Erwinia gerundensis</i> Strains Reveal Beneficial Early-Stage Plant–Bacteria InteractionsTongda Li0Ross Mann1Jatinder Kaur2German Spangenberg3Timothy Sawbridge4Agriculture Victoria, AgriBio, Centre for AgriBioscience, Bundoora, VIC 3083, AustraliaAgriculture Victoria, AgriBio, Centre for AgriBioscience, Bundoora, VIC 3083, AustraliaAgriculture Victoria, AgriBio, Centre for AgriBioscience, Bundoora, VIC 3083, AustraliaAgriculture Victoria, AgriBio, Centre for AgriBioscience, Bundoora, VIC 3083, AustraliaAgriculture Victoria, AgriBio, Centre for AgriBioscience, Bundoora, VIC 3083, AustraliaPlant growth-promoting bacteria can improve host plant traits including nutrient uptake and metabolism and tolerance to biotic and abiotic stresses. Understanding the molecular basis of plant–bacteria interactions using dual RNA-seq analyses provides key knowledge of both host and bacteria simultaneously, leading to future enhancements of beneficial interactions. In this study, dual RNA-seq analyses were performed to provide insights into the early-stage interactions between barley seedlings and three novel bacterial strains (two <i>Paenibacillus</i> sp. strains and one <i>Erwinia gerundensis</i> strain) isolated from the perennial ryegrass seed microbiome. Differentially expressed bacterial and barley genes/transcripts involved in plant–bacteria interactions were identified, with varying species- and strain-specific responses. Overall, transcriptome profiles suggested that all three strains improved stress response, signal transduction, and nutrient uptake and metabolism of barley seedlings. Results also suggested potential improvements in seedling root growth via repressing ethylene biosynthesis in roots. Bacterial secondary metabolite gene clusters producing compounds that are potentially associated with interactions with the barley endophytic microbiome and associated with stress tolerance of plants under nutrient limiting conditions were also identified. The results of this study provided the molecular basis of plant growth-promoting activities of three novel bacterial strains in barley, laid a solid foundation for the future development of these three bacterial strains as biofertilisers, and identified key differences between bacterial strains of the same species in their responses to plants.https://www.mdpi.com/2223-7747/10/9/1802<i>Paenibacillus</i><i>Erwinia gerundensis</i>barleygrowth-promotioninteractionRNA-seq |
| spellingShingle | Tongda Li Ross Mann Jatinder Kaur German Spangenberg Timothy Sawbridge Transcriptome Analyses of Barley Roots Inoculated with Novel <i>Paenibacillus</i> sp. and <i>Erwinia gerundensis</i> Strains Reveal Beneficial Early-Stage Plant–Bacteria Interactions Plants <i>Paenibacillus</i> <i>Erwinia gerundensis</i> barley growth-promotion interaction RNA-seq |
| title | Transcriptome Analyses of Barley Roots Inoculated with Novel <i>Paenibacillus</i> sp. and <i>Erwinia gerundensis</i> Strains Reveal Beneficial Early-Stage Plant–Bacteria Interactions |
| title_full | Transcriptome Analyses of Barley Roots Inoculated with Novel <i>Paenibacillus</i> sp. and <i>Erwinia gerundensis</i> Strains Reveal Beneficial Early-Stage Plant–Bacteria Interactions |
| title_fullStr | Transcriptome Analyses of Barley Roots Inoculated with Novel <i>Paenibacillus</i> sp. and <i>Erwinia gerundensis</i> Strains Reveal Beneficial Early-Stage Plant–Bacteria Interactions |
| title_full_unstemmed | Transcriptome Analyses of Barley Roots Inoculated with Novel <i>Paenibacillus</i> sp. and <i>Erwinia gerundensis</i> Strains Reveal Beneficial Early-Stage Plant–Bacteria Interactions |
| title_short | Transcriptome Analyses of Barley Roots Inoculated with Novel <i>Paenibacillus</i> sp. and <i>Erwinia gerundensis</i> Strains Reveal Beneficial Early-Stage Plant–Bacteria Interactions |
| title_sort | transcriptome analyses of barley roots inoculated with novel i paenibacillus i sp and i erwinia gerundensis i strains reveal beneficial early stage plant bacteria interactions |
| topic | <i>Paenibacillus</i> <i>Erwinia gerundensis</i> barley growth-promotion interaction RNA-seq |
| url | https://www.mdpi.com/2223-7747/10/9/1802 |
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