A Comprehensive Cytogenetic Analysis of Several Members of the Family Columbidae (Aves, Columbiformes)
The Columbidae species (Aves, Columbiformes) show considerable variation in their diploid numbers (2n = 68–86), but there is limited understanding of the events that shaped the extant karyotypes. Hence, we performed whole chromosome painting (wcp) for paints GGA1-10 and bacterial artificial chromoso...
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MDPI AG
2020-06-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/11/6/632 |
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| author | Rafael Kretschmer Ivanete de Oliveira Furo Anderson José Baia Gomes Lucas G. Kiazim Ricardo José Gunski Analía del Valle Garnero Jorge C. Pereira Malcolm A. Ferguson-Smith Edivaldo Herculano Corrêa de Oliveira Darren K. Griffin Thales Renato Ochotorena de Freitas Rebecca E. O’Connor |
| author_facet | Rafael Kretschmer Ivanete de Oliveira Furo Anderson José Baia Gomes Lucas G. Kiazim Ricardo José Gunski Analía del Valle Garnero Jorge C. Pereira Malcolm A. Ferguson-Smith Edivaldo Herculano Corrêa de Oliveira Darren K. Griffin Thales Renato Ochotorena de Freitas Rebecca E. O’Connor |
| author_sort | Rafael Kretschmer |
| collection | DOAJ |
| description | The Columbidae species (Aves, Columbiformes) show considerable variation in their diploid numbers (2n = 68–86), but there is limited understanding of the events that shaped the extant karyotypes. Hence, we performed whole chromosome painting (wcp) for paints GGA1-10 and bacterial artificial chromosome (BAC) probes for chromosomes GGA11-28 for <i>Columbina passerina</i>, <i>Columbina talpacoti</i>, <i>Patagioenas cayennensis</i>, <i>Geotrygon violacea</i> and <i>Geotrygon montana</i>. <i>Streptopelia decaocto</i> was only investigated with paints because BACs for GGA10-28 had been previously analyzed. We also performed phylogenetic analyses in order to trace the evolutionary history of this family in light of chromosomal changes using our wcp data with chicken probes and from <i>Zenaida auriculata</i>, <i>Columbina picui</i>, <i>Columba livia</i> and <i>Leptotila verreauxi</i>, previously published. G-banding was performed on all these species. Comparative chromosome paint and G-banding results suggested that at least one interchromosomal and many intrachromosomal rearrangements had occurred in the diversification of Columbidae species. On the other hand, a high degree of conservation of microchromosome organization was observed in these species. Our cladistic analysis, considering all the chromosome rearrangements detected, provided strong support for <i>L</i>. <i>verreauxi</i> and <i>P</i>. <i>cayennensis</i>, <i>G</i>. <i>montana</i> and <i>G</i>. <i>violacea</i>, <i>C</i>. <i>passerina</i> and <i>C</i>. <i>talpacoti</i> having sister taxa relationships, as well as for all Columbidae species analyzed herein. Additionally, the chromosome characters were mapped in a consensus phylogenetic topology previously proposed, revealing a pericentric inversion in the chromosome homologous to GGA4 in a chromosomal signature unique to small New World ground doves. |
| first_indexed | 2024-03-10T19:17:55Z |
| format | Article |
| id | doaj.art-6365f94ab7aa4ab9ab87ec09ab4e3071 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T19:17:55Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-6365f94ab7aa4ab9ab87ec09ab4e30712023-11-20T03:14:55ZengMDPI AGGenes2073-44252020-06-0111663210.3390/genes11060632A Comprehensive Cytogenetic Analysis of Several Members of the Family Columbidae (Aves, Columbiformes)Rafael Kretschmer0Ivanete de Oliveira Furo1Anderson José Baia Gomes2Lucas G. Kiazim3Ricardo José Gunski4Analía del Valle Garnero5Jorge C. Pereira6Malcolm A. Ferguson-Smith7Edivaldo Herculano Corrêa de Oliveira8Darren K. Griffin9Thales Renato Ochotorena de Freitas10Rebecca E. O’Connor11School of Biosciences, University of Kent, Canterbury CT2 7NJ, UKInstituto de Ciências Biológicas, Universidade Federal do Pará, Belém 66075-110, BrazilInstituto Federal do Pará, Abaetetuba 68440-000, BrazilSchool of Biosciences, University of Kent, Canterbury CT2 7NJ, UKLaboratório de Diversidade Genética Animal, Universidade Federal do Pampa, São Gabriel 97300-162, BrazilLaboratório de Diversidade Genética Animal, Universidade Federal do Pampa, São Gabriel 97300-162, BrazilAnimal and Veterinary Research Centre (CECAV), University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, PortugalCambridge Resource Centre for Comparative Genomics, University of Cambridge Department of Veterinary Medicine, Cambridge CB3 0ES, UKLaboratório de Cultura de Tecidos e Citogenética, SAMAM, Instituto Evandro Chagas, Ananindeua 67030-000, BrazilSchool of Biosciences, University of Kent, Canterbury CT2 7NJ, UKDepartamento de Genética, Universidade Federal do Rio Grande do Sul, Porto Alegre 91509-900, BrazilSchool of Biosciences, University of Kent, Canterbury CT2 7NJ, UKThe Columbidae species (Aves, Columbiformes) show considerable variation in their diploid numbers (2n = 68–86), but there is limited understanding of the events that shaped the extant karyotypes. Hence, we performed whole chromosome painting (wcp) for paints GGA1-10 and bacterial artificial chromosome (BAC) probes for chromosomes GGA11-28 for <i>Columbina passerina</i>, <i>Columbina talpacoti</i>, <i>Patagioenas cayennensis</i>, <i>Geotrygon violacea</i> and <i>Geotrygon montana</i>. <i>Streptopelia decaocto</i> was only investigated with paints because BACs for GGA10-28 had been previously analyzed. We also performed phylogenetic analyses in order to trace the evolutionary history of this family in light of chromosomal changes using our wcp data with chicken probes and from <i>Zenaida auriculata</i>, <i>Columbina picui</i>, <i>Columba livia</i> and <i>Leptotila verreauxi</i>, previously published. G-banding was performed on all these species. Comparative chromosome paint and G-banding results suggested that at least one interchromosomal and many intrachromosomal rearrangements had occurred in the diversification of Columbidae species. On the other hand, a high degree of conservation of microchromosome organization was observed in these species. Our cladistic analysis, considering all the chromosome rearrangements detected, provided strong support for <i>L</i>. <i>verreauxi</i> and <i>P</i>. <i>cayennensis</i>, <i>G</i>. <i>montana</i> and <i>G</i>. <i>violacea</i>, <i>C</i>. <i>passerina</i> and <i>C</i>. <i>talpacoti</i> having sister taxa relationships, as well as for all Columbidae species analyzed herein. Additionally, the chromosome characters were mapped in a consensus phylogenetic topology previously proposed, revealing a pericentric inversion in the chromosome homologous to GGA4 in a chromosomal signature unique to small New World ground doves.https://www.mdpi.com/2073-4425/11/6/632birdsdoves and pigeonsevolutiongenome organizationmacrochromosomesmicrochromosomes |
| spellingShingle | Rafael Kretschmer Ivanete de Oliveira Furo Anderson José Baia Gomes Lucas G. Kiazim Ricardo José Gunski Analía del Valle Garnero Jorge C. Pereira Malcolm A. Ferguson-Smith Edivaldo Herculano Corrêa de Oliveira Darren K. Griffin Thales Renato Ochotorena de Freitas Rebecca E. O’Connor A Comprehensive Cytogenetic Analysis of Several Members of the Family Columbidae (Aves, Columbiformes) Genes birds doves and pigeons evolution genome organization macrochromosomes microchromosomes |
| title | A Comprehensive Cytogenetic Analysis of Several Members of the Family Columbidae (Aves, Columbiformes) |
| title_full | A Comprehensive Cytogenetic Analysis of Several Members of the Family Columbidae (Aves, Columbiformes) |
| title_fullStr | A Comprehensive Cytogenetic Analysis of Several Members of the Family Columbidae (Aves, Columbiformes) |
| title_full_unstemmed | A Comprehensive Cytogenetic Analysis of Several Members of the Family Columbidae (Aves, Columbiformes) |
| title_short | A Comprehensive Cytogenetic Analysis of Several Members of the Family Columbidae (Aves, Columbiformes) |
| title_sort | comprehensive cytogenetic analysis of several members of the family columbidae aves columbiformes |
| topic | birds doves and pigeons evolution genome organization macrochromosomes microchromosomes |
| url | https://www.mdpi.com/2073-4425/11/6/632 |
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