Carbon dioxide quantified by the infrared in evaluation of respiratory activity of wheat seeds
ABSTRACT The objective of this study was to develop and validate the methodology of carbon dioxide concentration in the evaluation of vigor of Triticum aestivum L. seeds based on infrared spectroscopy. The proposed method quantifies CO2 content using a drag system and exhaustion to the gas released...
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Universidade Federal De Viçosa
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Series: | Revista Ceres |
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Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2017000500507&lng=en&tlng=en |
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author | João Alexandre Lopes Dranski Marlene de Matos Malavasi Ubirajara Contro Malavasi Ivan Schuster Norma Lazaretti |
author_facet | João Alexandre Lopes Dranski Marlene de Matos Malavasi Ubirajara Contro Malavasi Ivan Schuster Norma Lazaretti |
author_sort | João Alexandre Lopes Dranski |
collection | DOAJ |
description | ABSTRACT The objective of this study was to develop and validate the methodology of carbon dioxide concentration in the evaluation of vigor of Triticum aestivum L. seeds based on infrared spectroscopy. The proposed method quantifies CO2 content using a drag system and exhaustion to the gas released by the seeds. Samples of 50 seeds of six lots of cultivar CD 123 were incubated at temperatures of 15, 25 and 40 °C. The CO2 content released after 1, 3, 6, 9, 12 and 24 h of incubation were quantified. Additionally, the percentage of normal seedlings emerged in the field were evaluated. The simple correlation coefficients were evaluated among tests. After determining the reading conditions, the precision and accuracy of the proposed method were evaluated, using 15 lots of seeds. For the evaluation of the respiratory activity in Triticum aestivum seeds, a sample of 25 seeds, incubated at 25 °C for a minimum of 12 h is recommended since it allows to classify lots with different levels of vigor and predict the establishment of seedlings in the field, being the appropriate method for measuring CO2 as it externalizes precision between successive measurements and agreement with the reference method. |
first_indexed | 2024-04-13T12:56:33Z |
format | Article |
id | doaj.art-6369a80ab85445d4bf39692abf3cd825 |
institution | Directory Open Access Journal |
issn | 2177-3491 |
language | English |
last_indexed | 2024-04-13T12:56:33Z |
publisher | Universidade Federal De Viçosa |
record_format | Article |
series | Revista Ceres |
spelling | doaj.art-6369a80ab85445d4bf39692abf3cd8252022-12-22T02:46:01ZengUniversidade Federal De ViçosaRevista Ceres2177-349164550751510.1590/0034-737x201764050008S0034-737X2017000500507Carbon dioxide quantified by the infrared in evaluation of respiratory activity of wheat seedsJoão Alexandre Lopes DranskiMarlene de Matos MalavasiUbirajara Contro MalavasiIvan SchusterNorma LazarettiABSTRACT The objective of this study was to develop and validate the methodology of carbon dioxide concentration in the evaluation of vigor of Triticum aestivum L. seeds based on infrared spectroscopy. The proposed method quantifies CO2 content using a drag system and exhaustion to the gas released by the seeds. Samples of 50 seeds of six lots of cultivar CD 123 were incubated at temperatures of 15, 25 and 40 °C. The CO2 content released after 1, 3, 6, 9, 12 and 24 h of incubation were quantified. Additionally, the percentage of normal seedlings emerged in the field were evaluated. The simple correlation coefficients were evaluated among tests. After determining the reading conditions, the precision and accuracy of the proposed method were evaluated, using 15 lots of seeds. For the evaluation of the respiratory activity in Triticum aestivum seeds, a sample of 25 seeds, incubated at 25 °C for a minimum of 12 h is recommended since it allows to classify lots with different levels of vigor and predict the establishment of seedlings in the field, being the appropriate method for measuring CO2 as it externalizes precision between successive measurements and agreement with the reference method.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2017000500507&lng=en&tlng=enCO2infrared spectroscopyTriticum aestivum L.validation method |
spellingShingle | João Alexandre Lopes Dranski Marlene de Matos Malavasi Ubirajara Contro Malavasi Ivan Schuster Norma Lazaretti Carbon dioxide quantified by the infrared in evaluation of respiratory activity of wheat seeds Revista Ceres CO2 infrared spectroscopy Triticum aestivum L. validation method |
title | Carbon dioxide quantified by the infrared in evaluation of respiratory activity of wheat seeds |
title_full | Carbon dioxide quantified by the infrared in evaluation of respiratory activity of wheat seeds |
title_fullStr | Carbon dioxide quantified by the infrared in evaluation of respiratory activity of wheat seeds |
title_full_unstemmed | Carbon dioxide quantified by the infrared in evaluation of respiratory activity of wheat seeds |
title_short | Carbon dioxide quantified by the infrared in evaluation of respiratory activity of wheat seeds |
title_sort | carbon dioxide quantified by the infrared in evaluation of respiratory activity of wheat seeds |
topic | CO2 infrared spectroscopy Triticum aestivum L. validation method |
url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2017000500507&lng=en&tlng=en |
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