Carbon dioxide quantified by the infrared in evaluation of respiratory activity of wheat seeds

ABSTRACT The objective of this study was to develop and validate the methodology of carbon dioxide concentration in the evaluation of vigor of Triticum aestivum L. seeds based on infrared spectroscopy. The proposed method quantifies CO2 content using a drag system and exhaustion to the gas released...

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Main Authors: João Alexandre Lopes Dranski, Marlene de Matos Malavasi, Ubirajara Contro Malavasi, Ivan Schuster, Norma Lazaretti
Format: Article
Language:English
Published: Universidade Federal De Viçosa
Series:Revista Ceres
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2017000500507&lng=en&tlng=en
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author João Alexandre Lopes Dranski
Marlene de Matos Malavasi
Ubirajara Contro Malavasi
Ivan Schuster
Norma Lazaretti
author_facet João Alexandre Lopes Dranski
Marlene de Matos Malavasi
Ubirajara Contro Malavasi
Ivan Schuster
Norma Lazaretti
author_sort João Alexandre Lopes Dranski
collection DOAJ
description ABSTRACT The objective of this study was to develop and validate the methodology of carbon dioxide concentration in the evaluation of vigor of Triticum aestivum L. seeds based on infrared spectroscopy. The proposed method quantifies CO2 content using a drag system and exhaustion to the gas released by the seeds. Samples of 50 seeds of six lots of cultivar CD 123 were incubated at temperatures of 15, 25 and 40 °C. The CO2 content released after 1, 3, 6, 9, 12 and 24 h of incubation were quantified. Additionally, the percentage of normal seedlings emerged in the field were evaluated. The simple correlation coefficients were evaluated among tests. After determining the reading conditions, the precision and accuracy of the proposed method were evaluated, using 15 lots of seeds. For the evaluation of the respiratory activity in Triticum aestivum seeds, a sample of 25 seeds, incubated at 25 °C for a minimum of 12 h is recommended since it allows to classify lots with different levels of vigor and predict the establishment of seedlings in the field, being the appropriate method for measuring CO2 as it externalizes precision between successive measurements and agreement with the reference method.
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spelling doaj.art-6369a80ab85445d4bf39692abf3cd8252022-12-22T02:46:01ZengUniversidade Federal De ViçosaRevista Ceres2177-349164550751510.1590/0034-737x201764050008S0034-737X2017000500507Carbon dioxide quantified by the infrared in evaluation of respiratory activity of wheat seedsJoão Alexandre Lopes DranskiMarlene de Matos MalavasiUbirajara Contro MalavasiIvan SchusterNorma LazarettiABSTRACT The objective of this study was to develop and validate the methodology of carbon dioxide concentration in the evaluation of vigor of Triticum aestivum L. seeds based on infrared spectroscopy. The proposed method quantifies CO2 content using a drag system and exhaustion to the gas released by the seeds. Samples of 50 seeds of six lots of cultivar CD 123 were incubated at temperatures of 15, 25 and 40 °C. The CO2 content released after 1, 3, 6, 9, 12 and 24 h of incubation were quantified. Additionally, the percentage of normal seedlings emerged in the field were evaluated. The simple correlation coefficients were evaluated among tests. After determining the reading conditions, the precision and accuracy of the proposed method were evaluated, using 15 lots of seeds. For the evaluation of the respiratory activity in Triticum aestivum seeds, a sample of 25 seeds, incubated at 25 °C for a minimum of 12 h is recommended since it allows to classify lots with different levels of vigor and predict the establishment of seedlings in the field, being the appropriate method for measuring CO2 as it externalizes precision between successive measurements and agreement with the reference method.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2017000500507&lng=en&tlng=enCO2infrared spectroscopyTriticum aestivum L.validation method
spellingShingle João Alexandre Lopes Dranski
Marlene de Matos Malavasi
Ubirajara Contro Malavasi
Ivan Schuster
Norma Lazaretti
Carbon dioxide quantified by the infrared in evaluation of respiratory activity of wheat seeds
Revista Ceres
CO2
infrared spectroscopy
Triticum aestivum L.
validation method
title Carbon dioxide quantified by the infrared in evaluation of respiratory activity of wheat seeds
title_full Carbon dioxide quantified by the infrared in evaluation of respiratory activity of wheat seeds
title_fullStr Carbon dioxide quantified by the infrared in evaluation of respiratory activity of wheat seeds
title_full_unstemmed Carbon dioxide quantified by the infrared in evaluation of respiratory activity of wheat seeds
title_short Carbon dioxide quantified by the infrared in evaluation of respiratory activity of wheat seeds
title_sort carbon dioxide quantified by the infrared in evaluation of respiratory activity of wheat seeds
topic CO2
infrared spectroscopy
Triticum aestivum L.
validation method
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2017000500507&lng=en&tlng=en
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AT ubirajaracontromalavasi carbondioxidequantifiedbytheinfraredinevaluationofrespiratoryactivityofwheatseeds
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