Impedance testing of porous Si3N4 scaffolds for skeletal implant applications
Abstract Si3N4 ceramics show excellent characteristics of mechanical and chemical resistance in combination with good biocompatibility, antibacterial property and radiolucency. Therefore, they are intensively studied as structural materials in skeletal implant applications. Despite their attractive...
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| Format: | Article |
| Language: | English |
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Springer
2020-04-01
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| Series: | SN Applied Sciences |
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| Online Access: | https://doi.org/10.1007/s42452-020-2624-4 |
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| author | Serdar Onat Akbulut Hamed Ghorbanpoor Betül Özbek İpteç Adrian Butterworth Gamze Avcıoğlu Leyla Didem Kozacı Gülsüm Topateş Damion K. Corrigan Hüseyin Avcı Fatma D. Güzel |
| author_facet | Serdar Onat Akbulut Hamed Ghorbanpoor Betül Özbek İpteç Adrian Butterworth Gamze Avcıoğlu Leyla Didem Kozacı Gülsüm Topateş Damion K. Corrigan Hüseyin Avcı Fatma D. Güzel |
| author_sort | Serdar Onat Akbulut |
| collection | DOAJ |
| description | Abstract Si3N4 ceramics show excellent characteristics of mechanical and chemical resistance in combination with good biocompatibility, antibacterial property and radiolucency. Therefore, they are intensively studied as structural materials in skeletal implant applications. Despite their attractive properties, there are limited data in the field about in vitro studies of cellular growth on ceramic implant materials. In this study, the growth of bone cells was investigated on porous silicon nitride (Si3N4) ceramic implant by using electrochemical impedance spectroscopy (EIS). Partial sintering was performed at 1700 °C with limited amount of sintering additive for the production of porous Si3N4 scaffolds. All samples were then sterilized by using ethylene oxide followed by culturing MG-63 osteosarcoma cells on the substrates for in vitro assays. At 20 and 36 h, EIS was performed and results demonstrated that magnitude of the impedance as a result of the changes in the culture medium increased after incubation with osteosarcoma cells. The changes are attributed to the cellular uptake of charged molecules from the medium. Si3N4 samples appear to show large impedance magnitude changes, especially between 100 and 1 Hz. Impedance changes were also correlated with WST-1 measurements (36 h) and DAPI results. |
| first_indexed | 2024-12-17T22:56:02Z |
| format | Article |
| id | doaj.art-637066699ce148e0bf9e1549ccc4c241 |
| institution | Directory Open Access Journal |
| issn | 2523-3963 2523-3971 |
| language | English |
| last_indexed | 2024-12-17T22:56:02Z |
| publishDate | 2020-04-01 |
| publisher | Springer |
| record_format | Article |
| series | SN Applied Sciences |
| spelling | doaj.art-637066699ce148e0bf9e1549ccc4c2412022-12-21T21:29:33ZengSpringerSN Applied Sciences2523-39632523-39712020-04-01251610.1007/s42452-020-2624-4Impedance testing of porous Si3N4 scaffolds for skeletal implant applicationsSerdar Onat Akbulut0Hamed Ghorbanpoor1Betül Özbek İpteç2Adrian Butterworth3Gamze Avcıoğlu4Leyla Didem Kozacı5Gülsüm Topateş6Damion K. Corrigan7Hüseyin Avcı8Fatma D. Güzel9Metallurgical and Materials Engineering Department, Ankara Yildirim Beyazit UniversityDepartment of Biomedical Engineering, Ankara Yildirim Beyazit UniversityDepartment of Medical Biochemistry, Faculty of Medicine, Ankara Yildirim Beyazit UniversityDepartment of Biomedical Engineering, University of StrathclydeDepartment of Medical Biochemistry, Faculty of Medicine, Ankara Yildirim Beyazit UniversityDepartment of Medical Biochemistry, Faculty of Medicine, Ankara Yildirim Beyazit UniversityMetallurgical and Materials Engineering Department, Ankara Yildirim Beyazit UniversityDepartment of Biomedical Engineering, University of StrathclydeDepartment of Metallurgical and Materials Engineering, Eskisehir Osmangazi UniversityDepartment of Biomedical Engineering, Ankara Yildirim Beyazit UniversityAbstract Si3N4 ceramics show excellent characteristics of mechanical and chemical resistance in combination with good biocompatibility, antibacterial property and radiolucency. Therefore, they are intensively studied as structural materials in skeletal implant applications. Despite their attractive properties, there are limited data in the field about in vitro studies of cellular growth on ceramic implant materials. In this study, the growth of bone cells was investigated on porous silicon nitride (Si3N4) ceramic implant by using electrochemical impedance spectroscopy (EIS). Partial sintering was performed at 1700 °C with limited amount of sintering additive for the production of porous Si3N4 scaffolds. All samples were then sterilized by using ethylene oxide followed by culturing MG-63 osteosarcoma cells on the substrates for in vitro assays. At 20 and 36 h, EIS was performed and results demonstrated that magnitude of the impedance as a result of the changes in the culture medium increased after incubation with osteosarcoma cells. The changes are attributed to the cellular uptake of charged molecules from the medium. Si3N4 samples appear to show large impedance magnitude changes, especially between 100 and 1 Hz. Impedance changes were also correlated with WST-1 measurements (36 h) and DAPI results.https://doi.org/10.1007/s42452-020-2624-4Electrochemical impedance spectroscopyCellular attachmentBone implantSi3N4 |
| spellingShingle | Serdar Onat Akbulut Hamed Ghorbanpoor Betül Özbek İpteç Adrian Butterworth Gamze Avcıoğlu Leyla Didem Kozacı Gülsüm Topateş Damion K. Corrigan Hüseyin Avcı Fatma D. Güzel Impedance testing of porous Si3N4 scaffolds for skeletal implant applications SN Applied Sciences Electrochemical impedance spectroscopy Cellular attachment Bone implant Si3N4 |
| title | Impedance testing of porous Si3N4 scaffolds for skeletal implant applications |
| title_full | Impedance testing of porous Si3N4 scaffolds for skeletal implant applications |
| title_fullStr | Impedance testing of porous Si3N4 scaffolds for skeletal implant applications |
| title_full_unstemmed | Impedance testing of porous Si3N4 scaffolds for skeletal implant applications |
| title_short | Impedance testing of porous Si3N4 scaffolds for skeletal implant applications |
| title_sort | impedance testing of porous si3n4 scaffolds for skeletal implant applications |
| topic | Electrochemical impedance spectroscopy Cellular attachment Bone implant Si3N4 |
| url | https://doi.org/10.1007/s42452-020-2624-4 |
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