Regulation of feather follicle development and Msx2 gene SNP degradation in Hungarian white goose
Abstract Background Hungarian white goose has excellent down production performance and was introduced to China in 2010. The growth and development of feather follicles has an important impact on down production. Goose feather follicles can be divided into primary and secondary feather follicles, bo...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-12-01
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| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12864-022-09060-z |
| _version_ | 1828165655040360448 |
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| author | Yupu Song Chang Liu Yuxuan Zhou Guangyu Lin Chenguang Xu Petunia Msuthwana Sihui Wang Jingyun Ma Fangming Zhuang Xianou Fu Yudong Wang Tuoya Liu Qianyan Liu Jingbo Wang Yujian Sui Yongfeng Sun |
| author_facet | Yupu Song Chang Liu Yuxuan Zhou Guangyu Lin Chenguang Xu Petunia Msuthwana Sihui Wang Jingyun Ma Fangming Zhuang Xianou Fu Yudong Wang Tuoya Liu Qianyan Liu Jingbo Wang Yujian Sui Yongfeng Sun |
| author_sort | Yupu Song |
| collection | DOAJ |
| description | Abstract Background Hungarian white goose has excellent down production performance and was introduced to China in 2010. The growth and development of feather follicles has an important impact on down production. Goose feather follicles can be divided into primary and secondary feather follicles, both of which originate in the embryonic stage. Msx2 (Msh Homeobox 2) plays a regulatory role in tissues and organs such as eyes, teeth, bones and skin. However, its regulatory mechanism on goose feather follicles development remains unclear. Results Msx2 gene first increased, then decreased and increased at the end (E13, E18, E23, E28) during embryonic feather follicle development, and the expression level was the highest at E18. The pEGFP-N1-Msx2 overexpression vector and si-Msx2 siRNA vector were constructed to transfect goose embryo dermal fibroblasts. The results showed that the cell viability of ov-Msx2 group was significantly increased, and the gene expression levels of FGF5 and TGF-β1 genes were significantly down-regulated (P < 0.05), the expressions of PCNA, Bcl2, CDK1, FOXN1 and KGF genes were significantly up-regulated (P < 0.05). After transfection of siRNA vector, the cell viability of the si-Msx2 group was significantly decreased (P < 0.01) compared with the si-NC group. TGF-β1 expression was significantly up-regulated (P < 0.05), FGF5 expression was extremely significantly up-regulated (P < 0.01), while PCNA, Bcl2, CDK1, FOXN1 and KGF gene expression was significantly down-regulated (P < 0.05). High-throughput sequencing technology was used to mine the exon SNPs of Msx2. A total of 11 SNP loci were screened, four of the SNPs located in exon 1 were missense mutations. The feather follicle diameter of the GC genotype at the G78C site is significantly larger than that of the other two genotypes. Conclusions Msx2 maybe inhibit the apoptosis of goose dermal fibroblasts and promotes their proliferation. G78C can be used as a potential molecular marker for downy Variety. |
| first_indexed | 2024-04-12T01:48:19Z |
| format | Article |
| id | doaj.art-637228d582864d4d8ae476c129080727 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-12T01:48:19Z |
| publishDate | 2022-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-637228d582864d4d8ae476c1290807272022-12-22T03:53:01ZengBMCBMC Genomics1471-21642022-12-0123111210.1186/s12864-022-09060-zRegulation of feather follicle development and Msx2 gene SNP degradation in Hungarian white gooseYupu Song0Chang Liu1Yuxuan Zhou2Guangyu Lin3Chenguang Xu4Petunia Msuthwana5Sihui Wang6Jingyun Ma7Fangming Zhuang8Xianou Fu9Yudong Wang10Tuoya Liu11Qianyan Liu12Jingbo Wang13Yujian Sui14Yongfeng Sun15College of Animal Science and Technology, Jilin Agricultural UniversityChangchun Animal Husbandry ServiceCollege of Animal Science and Technology, Jilin Agricultural UniversityJilin Provincial Animal Husbandry Information CenterChangchun Animal Husbandry ServiceCollege of Animal Science and Technology, Jilin Agricultural UniversityCollege of Animal Science and Technology, Jilin Agricultural UniversityCollege of Animal Science and Technology, Jilin Agricultural UniversityCollege of Animal Science and Technology, Jilin Agricultural UniversityCollege of Animal Science and Technology, Jilin Agricultural UniversityCollege of Animal Science and Technology, Jilin Agricultural UniversityCollege of Animal Science and Technology, Jilin Agricultural UniversityCollege of Animal Science and Technology, Jilin Agricultural UniversityCollege of Animal Science and Technology, Jilin Agricultural UniversityCollege of Animal Science and Technology, Jilin Agricultural UniversityCollege of Animal Science and Technology, Jilin Agricultural UniversityAbstract Background Hungarian white goose has excellent down production performance and was introduced to China in 2010. The growth and development of feather follicles has an important impact on down production. Goose feather follicles can be divided into primary and secondary feather follicles, both of which originate in the embryonic stage. Msx2 (Msh Homeobox 2) plays a regulatory role in tissues and organs such as eyes, teeth, bones and skin. However, its regulatory mechanism on goose feather follicles development remains unclear. Results Msx2 gene first increased, then decreased and increased at the end (E13, E18, E23, E28) during embryonic feather follicle development, and the expression level was the highest at E18. The pEGFP-N1-Msx2 overexpression vector and si-Msx2 siRNA vector were constructed to transfect goose embryo dermal fibroblasts. The results showed that the cell viability of ov-Msx2 group was significantly increased, and the gene expression levels of FGF5 and TGF-β1 genes were significantly down-regulated (P < 0.05), the expressions of PCNA, Bcl2, CDK1, FOXN1 and KGF genes were significantly up-regulated (P < 0.05). After transfection of siRNA vector, the cell viability of the si-Msx2 group was significantly decreased (P < 0.01) compared with the si-NC group. TGF-β1 expression was significantly up-regulated (P < 0.05), FGF5 expression was extremely significantly up-regulated (P < 0.01), while PCNA, Bcl2, CDK1, FOXN1 and KGF gene expression was significantly down-regulated (P < 0.05). High-throughput sequencing technology was used to mine the exon SNPs of Msx2. A total of 11 SNP loci were screened, four of the SNPs located in exon 1 were missense mutations. The feather follicle diameter of the GC genotype at the G78C site is significantly larger than that of the other two genotypes. Conclusions Msx2 maybe inhibit the apoptosis of goose dermal fibroblasts and promotes their proliferation. G78C can be used as a potential molecular marker for downy Variety.https://doi.org/10.1186/s12864-022-09060-zHungarian white gooseFeather follicleGoose downSNP |
| spellingShingle | Yupu Song Chang Liu Yuxuan Zhou Guangyu Lin Chenguang Xu Petunia Msuthwana Sihui Wang Jingyun Ma Fangming Zhuang Xianou Fu Yudong Wang Tuoya Liu Qianyan Liu Jingbo Wang Yujian Sui Yongfeng Sun Regulation of feather follicle development and Msx2 gene SNP degradation in Hungarian white goose BMC Genomics Hungarian white goose Feather follicle Goose down SNP |
| title | Regulation of feather follicle development and Msx2 gene SNP degradation in Hungarian white goose |
| title_full | Regulation of feather follicle development and Msx2 gene SNP degradation in Hungarian white goose |
| title_fullStr | Regulation of feather follicle development and Msx2 gene SNP degradation in Hungarian white goose |
| title_full_unstemmed | Regulation of feather follicle development and Msx2 gene SNP degradation in Hungarian white goose |
| title_short | Regulation of feather follicle development and Msx2 gene SNP degradation in Hungarian white goose |
| title_sort | regulation of feather follicle development and msx2 gene snp degradation in hungarian white goose |
| topic | Hungarian white goose Feather follicle Goose down SNP |
| url | https://doi.org/10.1186/s12864-022-09060-z |
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