Downregulation of PITX2 inhibits the proliferation and migration of liver cancer cells and induces cell apoptosis
Paired-like homeodomain 2 (PITX2) functions as a transcription factor to participate in vertebrate embryogenesis, and dysregulated PITX2 expression was associated with the progression of various cancers. The functional role of PITX2 in tumorigenesis of liver cancer remains unknown. Western blot anal...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2021-12-01
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| Series: | Open Life Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/biol-2021-0133 |
| _version_ | 1818027497302786048 |
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| author | Tuerxun Kebinuer Zhang Shufang Zhang Yuexin |
| author_facet | Tuerxun Kebinuer Zhang Shufang Zhang Yuexin |
| author_sort | Tuerxun Kebinuer |
| collection | DOAJ |
| description | Paired-like homeodomain 2 (PITX2) functions as a transcription factor to participate in vertebrate embryogenesis, and dysregulated PITX2 expression was associated with the progression of various cancers. The functional role of PITX2 in tumorigenesis of liver cancer remains unknown. Western blot analysis showed that expression levels of PITX2 were enhanced in the liver cancer tissues and cells. siRNAs targeting PITX2 induced downregulation of PITX2 in liver cancer cells. siRNA-induced knockdown of PITX2 decreased liver cancer cell viability and proliferation, while promoting cell apoptosis by increasing cleaved-PARP, cleaved caspase 3, and cleaved caspase 9. The knockdown of PITX2 repressed liver cancer cell migration and invasion. In conclusion, elevated PITX2 expression was associated with liver cancer progression through repression of cell apoptosis and promoting cell proliferation and metastasis, and silencing of PITX2 might serve as a potential therapeutic strategy for the treatment of liver cancer. |
| first_indexed | 2024-12-10T04:48:50Z |
| format | Article |
| id | doaj.art-63748ea384684ac4812eaab0677d19ac |
| institution | Directory Open Access Journal |
| issn | 2391-5412 |
| language | English |
| last_indexed | 2024-12-10T04:48:50Z |
| publishDate | 2021-12-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Life Sciences |
| spelling | doaj.art-63748ea384684ac4812eaab0677d19ac2022-12-22T02:01:39ZengDe GruyterOpen Life Sciences2391-54122021-12-011611322132910.1515/biol-2021-0133Downregulation of PITX2 inhibits the proliferation and migration of liver cancer cells and induces cell apoptosisTuerxun Kebinuer0Zhang Shufang1Zhang Yuexin2Department of Infection and Liver Disease Center, The First Affiliated Hospital of Xinjiang Medical University, No. 137, Liyushan South Road, Urumqi, Xinjiang, 830054, ChinaDepartment of Infection and Liver Disease Center, The First Affiliated Hospital of Xinjiang Medical University, No. 137, Liyushan South Road, Urumqi, Xinjiang, 830054, ChinaDepartment of Infection and Liver Disease Center, The First Affiliated Hospital of Xinjiang Medical University, No. 137, Liyushan South Road, Urumqi, Xinjiang, 830054, ChinaPaired-like homeodomain 2 (PITX2) functions as a transcription factor to participate in vertebrate embryogenesis, and dysregulated PITX2 expression was associated with the progression of various cancers. The functional role of PITX2 in tumorigenesis of liver cancer remains unknown. Western blot analysis showed that expression levels of PITX2 were enhanced in the liver cancer tissues and cells. siRNAs targeting PITX2 induced downregulation of PITX2 in liver cancer cells. siRNA-induced knockdown of PITX2 decreased liver cancer cell viability and proliferation, while promoting cell apoptosis by increasing cleaved-PARP, cleaved caspase 3, and cleaved caspase 9. The knockdown of PITX2 repressed liver cancer cell migration and invasion. In conclusion, elevated PITX2 expression was associated with liver cancer progression through repression of cell apoptosis and promoting cell proliferation and metastasis, and silencing of PITX2 might serve as a potential therapeutic strategy for the treatment of liver cancer.https://doi.org/10.1515/biol-2021-0133pitx2liver cancerproliferationmetastasis |
| spellingShingle | Tuerxun Kebinuer Zhang Shufang Zhang Yuexin Downregulation of PITX2 inhibits the proliferation and migration of liver cancer cells and induces cell apoptosis Open Life Sciences pitx2 liver cancer proliferation metastasis |
| title | Downregulation of PITX2 inhibits the proliferation and migration of liver cancer cells and induces cell apoptosis |
| title_full | Downregulation of PITX2 inhibits the proliferation and migration of liver cancer cells and induces cell apoptosis |
| title_fullStr | Downregulation of PITX2 inhibits the proliferation and migration of liver cancer cells and induces cell apoptosis |
| title_full_unstemmed | Downregulation of PITX2 inhibits the proliferation and migration of liver cancer cells and induces cell apoptosis |
| title_short | Downregulation of PITX2 inhibits the proliferation and migration of liver cancer cells and induces cell apoptosis |
| title_sort | downregulation of pitx2 inhibits the proliferation and migration of liver cancer cells and induces cell apoptosis |
| topic | pitx2 liver cancer proliferation metastasis |
| url | https://doi.org/10.1515/biol-2021-0133 |
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