Induction of Laccase, Lignin Peroxidase and Manganese Peroxidase Activities in White-Rot Fungi Using Copper Complexes
Ligninolytic enzymes, such as laccase, lignin peroxidase and manganese peroxidase, are biotechnologically-important enzymes. The ability of five white-rot fungal strains Daedaleopsis confragosa, Fomes fomentarius, Trametes gibbosa, Trametes suaveolens and Trametes versicolor to produce these enzymes...
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MDPI AG
2016-11-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/21/11/1553 |
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| author | Martina Vrsanska Stanislava Voberkova Vratislav Langer Dagmar Palovcikova Amitava Moulick Vojtech Adam Pavel Kopel |
| author_facet | Martina Vrsanska Stanislava Voberkova Vratislav Langer Dagmar Palovcikova Amitava Moulick Vojtech Adam Pavel Kopel |
| author_sort | Martina Vrsanska |
| collection | DOAJ |
| description | Ligninolytic enzymes, such as laccase, lignin peroxidase and manganese peroxidase, are biotechnologically-important enzymes. The ability of five white-rot fungal strains Daedaleopsis confragosa, Fomes fomentarius, Trametes gibbosa, Trametes suaveolens and Trametes versicolor to produce these enzymes has been studied. Three different copper(II) complexes have been prepared ((Him)[Cu(im)4(H2O)2](btc)·3H2O, where im = imidazole, H3btc = 1,3,5-benzenetricarboxylic acid, [Cu3(pmdien)3(btc)](ClO4)3·6H2O) and [Cu3(mdpta)3(btc)](ClO4)3·4H2O, where pmdien = N,N,N′,N′′,N′′-pentamethyl-diethylenetriamine and mdpta = N,N-bis-(3-aminopropyl)methyl- amine), and their potential application for laccase and peroxidases induction have been tested. The enzyme-inducing activities of the complexes were compared with that of copper sulfate, and it has been found that all of the complexes are suitable for the induction of laccase and peroxidase activities in white-rot fungi; however, the newly-synthesized complex M1 showed the greatest potential for the induction. With respect to the different copper inducers, this parameter seems to be important for enzyme activity, which depends also on the fungal strains. |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-13T09:11:15Z |
| publishDate | 2016-11-01 |
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| series | Molecules |
| spelling | doaj.art-6375f9eae9944d3e86e9144bc48b905b2022-12-22T02:52:53ZengMDPI AGMolecules1420-30492016-11-012111155310.3390/molecules21111553molecules21111553Induction of Laccase, Lignin Peroxidase and Manganese Peroxidase Activities in White-Rot Fungi Using Copper ComplexesMartina Vrsanska0Stanislava Voberkova1Vratislav Langer2Dagmar Palovcikova3Amitava Moulick4Vojtech Adam5Pavel Kopel6Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech RepublicDepartment of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech RepublicEnvironmental Inorganic Chemistry, Department of Chemical and Biological Engineering, Chalmers University of Technology, SE-412 96 Göteborg, SwedenDepartment of Forest Protection and Wildlife Management, Faculty of Forestry and Wood Technology, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech RepublicDepartment of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech RepublicDepartment of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech RepublicDepartment of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech RepublicLigninolytic enzymes, such as laccase, lignin peroxidase and manganese peroxidase, are biotechnologically-important enzymes. The ability of five white-rot fungal strains Daedaleopsis confragosa, Fomes fomentarius, Trametes gibbosa, Trametes suaveolens and Trametes versicolor to produce these enzymes has been studied. Three different copper(II) complexes have been prepared ((Him)[Cu(im)4(H2O)2](btc)·3H2O, where im = imidazole, H3btc = 1,3,5-benzenetricarboxylic acid, [Cu3(pmdien)3(btc)](ClO4)3·6H2O) and [Cu3(mdpta)3(btc)](ClO4)3·4H2O, where pmdien = N,N,N′,N′′,N′′-pentamethyl-diethylenetriamine and mdpta = N,N-bis-(3-aminopropyl)methyl- amine), and their potential application for laccase and peroxidases induction have been tested. The enzyme-inducing activities of the complexes were compared with that of copper sulfate, and it has been found that all of the complexes are suitable for the induction of laccase and peroxidase activities in white-rot fungi; however, the newly-synthesized complex M1 showed the greatest potential for the induction. With respect to the different copper inducers, this parameter seems to be important for enzyme activity, which depends also on the fungal strains.http://www.mdpi.com/1420-3049/21/11/1553laccaseperoxidasecopper complextrimesic acidenzymatic induction |
| spellingShingle | Martina Vrsanska Stanislava Voberkova Vratislav Langer Dagmar Palovcikova Amitava Moulick Vojtech Adam Pavel Kopel Induction of Laccase, Lignin Peroxidase and Manganese Peroxidase Activities in White-Rot Fungi Using Copper Complexes Molecules laccase peroxidase copper complex trimesic acid enzymatic induction |
| title | Induction of Laccase, Lignin Peroxidase and Manganese Peroxidase Activities in White-Rot Fungi Using Copper Complexes |
| title_full | Induction of Laccase, Lignin Peroxidase and Manganese Peroxidase Activities in White-Rot Fungi Using Copper Complexes |
| title_fullStr | Induction of Laccase, Lignin Peroxidase and Manganese Peroxidase Activities in White-Rot Fungi Using Copper Complexes |
| title_full_unstemmed | Induction of Laccase, Lignin Peroxidase and Manganese Peroxidase Activities in White-Rot Fungi Using Copper Complexes |
| title_short | Induction of Laccase, Lignin Peroxidase and Manganese Peroxidase Activities in White-Rot Fungi Using Copper Complexes |
| title_sort | induction of laccase lignin peroxidase and manganese peroxidase activities in white rot fungi using copper complexes |
| topic | laccase peroxidase copper complex trimesic acid enzymatic induction |
| url | http://www.mdpi.com/1420-3049/21/11/1553 |
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