Biochemistry of microbial itaconic acid production
Itaconic acid is an unsatured dicarbonic acid which has a high potential as a biochemical building block, because it can be used as a monomer for the production of a plethora of products including resins, plastics, paints and synthetic fibers. Some Aspergillus species, like A. itaconicus and A. terr...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2013-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2013.00023/full |
| _version_ | 1818556892242247680 |
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| author | Matthias Georg Steiger Matthias Georg Steiger Marzena Lidia Blumhoff Marzena Lidia Blumhoff Marzena Lidia Blumhoff Diethard eMattanovich Diethard eMattanovich Michael eSauer Michael eSauer |
| author_facet | Matthias Georg Steiger Matthias Georg Steiger Marzena Lidia Blumhoff Marzena Lidia Blumhoff Marzena Lidia Blumhoff Diethard eMattanovich Diethard eMattanovich Michael eSauer Michael eSauer |
| author_sort | Matthias Georg Steiger |
| collection | DOAJ |
| description | Itaconic acid is an unsatured dicarbonic acid which has a high potential as a biochemical building block, because it can be used as a monomer for the production of a plethora of products including resins, plastics, paints and synthetic fibers. Some Aspergillus species, like A. itaconicus and A. terreus, show the ability to synthesize this organic acid and A. terreus can secrete significant amounts to the media (> 80 g/L). However, compared with the citric acid production process (titers > 200 g/L) the achieved titers are still low and the overall process is expensive because purified substrates are required for optimal productivity. Itaconate is formed by the enzymatic activity of a cis-aconitate decarboxylase (CadA) encoded by the cadA gene in A. terreus. Cloning of the cadA gene into the citric acid producing fungus A. niger showed that it is possible to produce itaconic acid also in a different host organism. This review will describe the current status and recent advances in the understanding of the molecular processes leading to the biotechnological production of itaconic acid. |
| first_indexed | 2024-12-13T23:53:05Z |
| format | Article |
| id | doaj.art-63798802865842f9aaefea81684451e0 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-13T23:53:05Z |
| publishDate | 2013-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-63798802865842f9aaefea81684451e02022-12-21T23:26:42ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2013-02-01410.3389/fmicb.2013.0002344399Biochemistry of microbial itaconic acid productionMatthias Georg Steiger0Matthias Georg Steiger1Marzena Lidia Blumhoff2Marzena Lidia Blumhoff3Marzena Lidia Blumhoff4Diethard eMattanovich5Diethard eMattanovich6Michael eSauer7Michael eSauer8Austrian Centre of Industrial Biotechnology GmbHBOKU – VIBT University of Natural Resources and Life SciencesAustrian Centre of Industrial Biotechnology GmbHBOKU – VIBT University of Natural Resources and Life SciencesUniversity of Applied Sciences FH-Campus WienAustrian Centre of Industrial Biotechnology GmbHBOKU – VIBT University of Natural Resources and Life SciencesAustrian Centre of Industrial Biotechnology GmbHBOKU – VIBT University of Natural Resources and Life SciencesItaconic acid is an unsatured dicarbonic acid which has a high potential as a biochemical building block, because it can be used as a monomer for the production of a plethora of products including resins, plastics, paints and synthetic fibers. Some Aspergillus species, like A. itaconicus and A. terreus, show the ability to synthesize this organic acid and A. terreus can secrete significant amounts to the media (> 80 g/L). However, compared with the citric acid production process (titers > 200 g/L) the achieved titers are still low and the overall process is expensive because purified substrates are required for optimal productivity. Itaconate is formed by the enzymatic activity of a cis-aconitate decarboxylase (CadA) encoded by the cadA gene in A. terreus. Cloning of the cadA gene into the citric acid producing fungus A. niger showed that it is possible to produce itaconic acid also in a different host organism. This review will describe the current status and recent advances in the understanding of the molecular processes leading to the biotechnological production of itaconic acid.http://journal.frontiersin.org/Journal/10.3389/fmicb.2013.00023/fullAspergillus nigerIndustrial MicrobiologyMetabolic Engineeringcis-aconitic acid decarboxylaseAspergillus terreusbiochemical pathways |
| spellingShingle | Matthias Georg Steiger Matthias Georg Steiger Marzena Lidia Blumhoff Marzena Lidia Blumhoff Marzena Lidia Blumhoff Diethard eMattanovich Diethard eMattanovich Michael eSauer Michael eSauer Biochemistry of microbial itaconic acid production Frontiers in Microbiology Aspergillus niger Industrial Microbiology Metabolic Engineering cis-aconitic acid decarboxylase Aspergillus terreus biochemical pathways |
| title | Biochemistry of microbial itaconic acid production |
| title_full | Biochemistry of microbial itaconic acid production |
| title_fullStr | Biochemistry of microbial itaconic acid production |
| title_full_unstemmed | Biochemistry of microbial itaconic acid production |
| title_short | Biochemistry of microbial itaconic acid production |
| title_sort | biochemistry of microbial itaconic acid production |
| topic | Aspergillus niger Industrial Microbiology Metabolic Engineering cis-aconitic acid decarboxylase Aspergillus terreus biochemical pathways |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2013.00023/full |
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