ECIS Based Electric Fence Method for Measurement of Human Keratinocyte Migration on Different Substrates
Electric Cell-substrate Impedance Sensing (ECIS) is an impedance-based, real-time, and label-free measuring system for monitoring cellular activities in tissue culture. Previously, ECIS wound healing assay has been used to wound cells with high electric current and monitor the subsequent cell migrat...
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MDPI AG
2022-05-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/5/293 |
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| author | Yu-Han Hung Wei-Chih Chiu Shyh-Rong Fuh Yi-Ting Lai Tse-Hua Tung Chun-Chung Huang Chun-Min Lo |
| author_facet | Yu-Han Hung Wei-Chih Chiu Shyh-Rong Fuh Yi-Ting Lai Tse-Hua Tung Chun-Chung Huang Chun-Min Lo |
| author_sort | Yu-Han Hung |
| collection | DOAJ |
| description | Electric Cell-substrate Impedance Sensing (ECIS) is an impedance-based, real-time, and label-free measuring system for monitoring cellular activities in tissue culture. Previously, ECIS wound healing assay has been used to wound cells with high electric current and monitor the subsequent cell migration. In this study, we applied ECIS electric fence (EF) method, an alternative to electrical wounding, to assess the effects of different surface coatings on human keratinocyte (HaCaT) migration. The EF prevents inoculated cells from attaching or migrating to the fenced electrode surface while maintaining the integrity of the surface coating. After the EF is turned off, cells migrate into the cell-free area, and the increase in measured impedance is monitored. We cultured HaCaT cells on gold electrodes without coating or coated with poly-L-lysin (PLL), poly-D-lysine (PDL), or type-I collagen. We quantified migration rates according to the different slopes in the impedance time series. It was observed that either poly-L-lysine (PLL) or poly-D-lysine (PDL) limits cell adhesion and migration rates. Furthermore, the surface charge of the coated substrate in the culture condition positively correlates with the cell adhesion and migration process. Our results indicate that the EF method is useful for determining cell migration rates on specific surface coatings. |
| first_indexed | 2024-03-10T03:15:32Z |
| format | Article |
| id | doaj.art-637ac3e8825945e6beabaacb52d4e8c2 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T03:15:32Z |
| publishDate | 2022-05-01 |
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| spelling | doaj.art-637ac3e8825945e6beabaacb52d4e8c22023-11-23T10:15:25ZengMDPI AGBiosensors2079-63742022-05-0112529310.3390/bios12050293ECIS Based Electric Fence Method for Measurement of Human Keratinocyte Migration on Different SubstratesYu-Han Hung0Wei-Chih Chiu1Shyh-Rong Fuh2Yi-Ting Lai3Tse-Hua Tung4Chun-Chung Huang5Chun-Min Lo6Department of Biomedical Engineering, National Yang Ming Chiao Tung University, Taipei 112, TaiwanDepartment of Biomedical Engineering, National Yang Ming Chiao Tung University, Taipei 112, TaiwanDepartment of Biomedical Engineering, National Yang Ming Chiao Tung University, Taipei 112, TaiwanDepartment of Biomedical Engineering, National Yang Ming Chiao Tung University, Taipei 112, TaiwanDepartment of Biomedical Engineering, National Yang Ming Chiao Tung University, Taipei 112, TaiwanDepartment of Biomedical Engineering, National Yang Ming Chiao Tung University, Taipei 112, TaiwanDepartment of Biomedical Engineering, National Yang Ming Chiao Tung University, Taipei 112, TaiwanElectric Cell-substrate Impedance Sensing (ECIS) is an impedance-based, real-time, and label-free measuring system for monitoring cellular activities in tissue culture. Previously, ECIS wound healing assay has been used to wound cells with high electric current and monitor the subsequent cell migration. In this study, we applied ECIS electric fence (EF) method, an alternative to electrical wounding, to assess the effects of different surface coatings on human keratinocyte (HaCaT) migration. The EF prevents inoculated cells from attaching or migrating to the fenced electrode surface while maintaining the integrity of the surface coating. After the EF is turned off, cells migrate into the cell-free area, and the increase in measured impedance is monitored. We cultured HaCaT cells on gold electrodes without coating or coated with poly-L-lysin (PLL), poly-D-lysine (PDL), or type-I collagen. We quantified migration rates according to the different slopes in the impedance time series. It was observed that either poly-L-lysine (PLL) or poly-D-lysine (PDL) limits cell adhesion and migration rates. Furthermore, the surface charge of the coated substrate in the culture condition positively correlates with the cell adhesion and migration process. Our results indicate that the EF method is useful for determining cell migration rates on specific surface coatings.https://www.mdpi.com/2079-6374/12/5/293cell adhesioncell migrationECISelectric fence (EF)wound healing assays |
| spellingShingle | Yu-Han Hung Wei-Chih Chiu Shyh-Rong Fuh Yi-Ting Lai Tse-Hua Tung Chun-Chung Huang Chun-Min Lo ECIS Based Electric Fence Method for Measurement of Human Keratinocyte Migration on Different Substrates Biosensors cell adhesion cell migration ECIS electric fence (EF) wound healing assays |
| title | ECIS Based Electric Fence Method for Measurement of Human Keratinocyte Migration on Different Substrates |
| title_full | ECIS Based Electric Fence Method for Measurement of Human Keratinocyte Migration on Different Substrates |
| title_fullStr | ECIS Based Electric Fence Method for Measurement of Human Keratinocyte Migration on Different Substrates |
| title_full_unstemmed | ECIS Based Electric Fence Method for Measurement of Human Keratinocyte Migration on Different Substrates |
| title_short | ECIS Based Electric Fence Method for Measurement of Human Keratinocyte Migration on Different Substrates |
| title_sort | ecis based electric fence method for measurement of human keratinocyte migration on different substrates |
| topic | cell adhesion cell migration ECIS electric fence (EF) wound healing assays |
| url | https://www.mdpi.com/2079-6374/12/5/293 |
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