Pathogenetic detection, retrospective and pathogenicity analysis of a fatal case of Vibrio vulnificus in Shenzhen, China
Abstract We report a 36-year-old male patient died of V. vulnificus-induced septicaemia and multiple organ failure syndrome after oyster consumption at a restaurant. We isolated and identified V. vulnificus vv16015 from the patient’s blood sample and antibiotic susceptibility tests indicated sensiti...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-11-01
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| Series: | Gut Pathogens |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13099-023-00580-x |
| _version_ | 1797452513410023424 |
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| author | Shiqin Xu Jinsong Wu Ying Jin Liyin Ji Xuan Zou Qinghua Hu Tiejian Feng Shuang Wu Yixiang Jiang Qiongcheng Chen Huiqun Lu Shuxiang Qiu Huaisheng Chen Min Jiang Rui Cai Yaqun Qiu Xiaolu Shi |
| author_facet | Shiqin Xu Jinsong Wu Ying Jin Liyin Ji Xuan Zou Qinghua Hu Tiejian Feng Shuang Wu Yixiang Jiang Qiongcheng Chen Huiqun Lu Shuxiang Qiu Huaisheng Chen Min Jiang Rui Cai Yaqun Qiu Xiaolu Shi |
| author_sort | Shiqin Xu |
| collection | DOAJ |
| description | Abstract We report a 36-year-old male patient died of V. vulnificus-induced septicaemia and multiple organ failure syndrome after oyster consumption at a restaurant. We isolated and identified V. vulnificus vv16015 from the patient’s blood sample and antibiotic susceptibility tests indicated sensitivity to all 21 antibiotics. Oyster samples were subsequently collected from the restaurant’s supplier and three strains of V. vulnificus were isolated. Whole genome sequencing and analysis revealed vv16015 to be distantly related to these strains and confirmed that V. vulnificus contamination was present in the seafood of the restaurant and supplier. Using a Galleria mellonella larvae infection model, the virulence of vv16015 was determined to be higher than that of comparison strains isolated from a surviving patient (vv15018) and an oyster (vv220015). The human and environment distribution of V. vulnificus in Shenzhen is sporadic and heterogeneous, and vv16015 is highly virulent compared to other strains. |
| first_indexed | 2024-03-09T15:09:46Z |
| format | Article |
| id | doaj.art-637bee6a599942bd8be3741ac0a88a72 |
| institution | Directory Open Access Journal |
| issn | 1757-4749 |
| language | English |
| last_indexed | 2024-03-09T15:09:46Z |
| publishDate | 2023-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Gut Pathogens |
| spelling | doaj.art-637bee6a599942bd8be3741ac0a88a722023-11-26T13:27:24ZengBMCGut Pathogens1757-47492023-11-011511610.1186/s13099-023-00580-xPathogenetic detection, retrospective and pathogenicity analysis of a fatal case of Vibrio vulnificus in Shenzhen, ChinaShiqin Xu0Jinsong Wu1Ying Jin2Liyin Ji3Xuan Zou4Qinghua Hu5Tiejian Feng6Shuang Wu7Yixiang Jiang8Qiongcheng Chen9Huiqun Lu10Shuxiang Qiu11Huaisheng Chen12Min Jiang13Rui Cai14Yaqun Qiu15Xiaolu Shi16School of Public Health, Shanxi Medical UniversityShenzhen People’s HospitalShenzhen Bao’an District Songgang People’s HospitalSchool of Public Health, Shanxi Medical UniversityShenzhen Center for Disease Control and PreventionShenzhen Center for Disease Control and PreventionShenzhen Center for Disease Control and PreventionShenzhen Center for Disease Control and PreventionShenzhen Center for Disease Control and PreventionShenzhen Center for Disease Control and PreventionSchool of Public Health, University of South ChinaSchool of Public Health, University of South ChinaShenzhen People’s HospitalShenzhen Center for Disease Control and PreventionShenzhen Center for Disease Control and PreventionShenzhen Center for Disease Control and PreventionSchool of Public Health, Shanxi Medical UniversityAbstract We report a 36-year-old male patient died of V. vulnificus-induced septicaemia and multiple organ failure syndrome after oyster consumption at a restaurant. We isolated and identified V. vulnificus vv16015 from the patient’s blood sample and antibiotic susceptibility tests indicated sensitivity to all 21 antibiotics. Oyster samples were subsequently collected from the restaurant’s supplier and three strains of V. vulnificus were isolated. Whole genome sequencing and analysis revealed vv16015 to be distantly related to these strains and confirmed that V. vulnificus contamination was present in the seafood of the restaurant and supplier. Using a Galleria mellonella larvae infection model, the virulence of vv16015 was determined to be higher than that of comparison strains isolated from a surviving patient (vv15018) and an oyster (vv220015). The human and environment distribution of V. vulnificus in Shenzhen is sporadic and heterogeneous, and vv16015 is highly virulent compared to other strains.https://doi.org/10.1186/s13099-023-00580-xVibrio vulnificusFood PoisoningAntibiotic susceptibilityVirulence testingGenome sequencing |
| spellingShingle | Shiqin Xu Jinsong Wu Ying Jin Liyin Ji Xuan Zou Qinghua Hu Tiejian Feng Shuang Wu Yixiang Jiang Qiongcheng Chen Huiqun Lu Shuxiang Qiu Huaisheng Chen Min Jiang Rui Cai Yaqun Qiu Xiaolu Shi Pathogenetic detection, retrospective and pathogenicity analysis of a fatal case of Vibrio vulnificus in Shenzhen, China Gut Pathogens Vibrio vulnificus Food Poisoning Antibiotic susceptibility Virulence testing Genome sequencing |
| title | Pathogenetic detection, retrospective and pathogenicity analysis of a fatal case of Vibrio vulnificus in Shenzhen, China |
| title_full | Pathogenetic detection, retrospective and pathogenicity analysis of a fatal case of Vibrio vulnificus in Shenzhen, China |
| title_fullStr | Pathogenetic detection, retrospective and pathogenicity analysis of a fatal case of Vibrio vulnificus in Shenzhen, China |
| title_full_unstemmed | Pathogenetic detection, retrospective and pathogenicity analysis of a fatal case of Vibrio vulnificus in Shenzhen, China |
| title_short | Pathogenetic detection, retrospective and pathogenicity analysis of a fatal case of Vibrio vulnificus in Shenzhen, China |
| title_sort | pathogenetic detection retrospective and pathogenicity analysis of a fatal case of vibrio vulnificus in shenzhen china |
| topic | Vibrio vulnificus Food Poisoning Antibiotic susceptibility Virulence testing Genome sequencing |
| url | https://doi.org/10.1186/s13099-023-00580-x |
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