The Composition, Diversity and Predictive Metabolic Profiles of Bacteria Associated With the Gut Digesta of Five Sea Urchins in Luhuitou Fringing Reef (Northern South China Sea)
Sea urchins strongly affect reef ecology, and the bacteria associated with their gut digesta have not been well studied in coral reefs. In the current study, we analyze the bacterial composition of five sea urchin species collected from Luhuitou fringing reef, namely Stomopneustes variolaris, Diadem...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.01168/full |
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| author | Qiucui Yao Qiucui Yao Qiucui Yao Qiucui Yao Qiucui Yao Kefu Yu Kefu Yu Kefu Yu Jiayuan Liang Jiayuan Liang Jiayuan Liang Yinghui Wang Yinghui Wang Yinghui Wang Baoqing Hu Xueyong Huang Xueyong Huang Xueyong Huang Biao Chen Biao Chen Biao Chen Zhenjun Qin Zhenjun Qin Zhenjun Qin |
| author_facet | Qiucui Yao Qiucui Yao Qiucui Yao Qiucui Yao Qiucui Yao Kefu Yu Kefu Yu Kefu Yu Jiayuan Liang Jiayuan Liang Jiayuan Liang Yinghui Wang Yinghui Wang Yinghui Wang Baoqing Hu Xueyong Huang Xueyong Huang Xueyong Huang Biao Chen Biao Chen Biao Chen Zhenjun Qin Zhenjun Qin Zhenjun Qin |
| author_sort | Qiucui Yao |
| collection | DOAJ |
| description | Sea urchins strongly affect reef ecology, and the bacteria associated with their gut digesta have not been well studied in coral reefs. In the current study, we analyze the bacterial composition of five sea urchin species collected from Luhuitou fringing reef, namely Stomopneustes variolaris, Diadema setosum, Echinothrix calamaris, Diadema savignyi, and Tripneustes gratilla, using high-throughput 16S rRNA gene-based pyrosequencing. Propionigenium, Prolixibacter, and Photobacterium were found to be the dominant bacterial genera in all five species. Interestingly, four sea urchin species, including S. variolaris, D. setosum, E. calamaris, and D. savignyi, displayed a higher mean total abundance of the three bacterial genera (69.72 ± 6.49%) than T. gratilla (43.37 ± 13.47%). Diversity analysis indicated that the gut digesta of sea urchin T. gratilla displayed a higher bacterial α-diversity compared with the other four species. PCoA showed that the four groups representing D. setosum, D. savignyi, E. calamaris, and S. variolaris were overlapping, but distant from the group representing T. gratilla. Predictive metagenomics performed by PICRUSt revealed that the abundances of genes involved in amino acid metabolism and metabolism of terpenoid and polyketide were higher in T. gratilla, while those involved in carbohydrate metabolism were higher in the other four sea urchin species. Therefore, our results indicated that the composition, diversity and predictive metabolic profiles of bacteria associated with the gut digesta of T. gratilla were significantly different from those of the other four sea urchin species in Luhuitou fringing reef. |
| first_indexed | 2024-12-23T06:57:28Z |
| format | Article |
| id | doaj.art-637d079d022944caaf9a08f67391768c |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-23T06:57:28Z |
| publishDate | 2019-05-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-637d079d022944caaf9a08f67391768c2022-12-21T17:56:17ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-05-011010.3389/fmicb.2019.01168429972The Composition, Diversity and Predictive Metabolic Profiles of Bacteria Associated With the Gut Digesta of Five Sea Urchins in Luhuitou Fringing Reef (Northern South China Sea)Qiucui Yao0Qiucui Yao1Qiucui Yao2Qiucui Yao3Qiucui Yao4Kefu Yu5Kefu Yu6Kefu Yu7Jiayuan Liang8Jiayuan Liang9Jiayuan Liang10Yinghui Wang11Yinghui Wang12Yinghui Wang13Baoqing Hu14Xueyong Huang15Xueyong Huang16Xueyong Huang17Biao Chen18Biao Chen19Biao Chen20Zhenjun Qin21Zhenjun Qin22Zhenjun Qin23Coral Reef Research Center of China, Guangxi University, Nanning, ChinaGuangxi Laboratory on the Study of Coral Reefs in the South China Sea, Guangxi University, Nanning, ChinaSchool of Marine Sciences, Guangxi University, Nanning, ChinaCollege of Forestry, Guangxi University, Nanning, ChinaKey Laboratory of Environment Change and Resources Use in Beibu Gulf, Ministry of Education, Guangxi Teachers Education University, Nanning, ChinaCoral Reef Research Center of China, Guangxi University, Nanning, ChinaGuangxi Laboratory on the Study of Coral Reefs in the South China Sea, Guangxi University, Nanning, ChinaSchool of Marine Sciences, Guangxi University, Nanning, ChinaCoral Reef Research Center of China, Guangxi University, Nanning, ChinaGuangxi Laboratory on the Study of Coral Reefs in the South China Sea, Guangxi University, Nanning, ChinaSchool of Marine Sciences, Guangxi University, Nanning, ChinaCoral Reef Research Center of China, Guangxi University, Nanning, ChinaGuangxi Laboratory on the Study of Coral Reefs in the South China Sea, Guangxi University, Nanning, ChinaSchool of Marine Sciences, Guangxi University, Nanning, ChinaKey Laboratory of Environment Change and Resources Use in Beibu Gulf, Ministry of Education, Guangxi Teachers Education University, Nanning, ChinaCoral Reef Research Center of China, Guangxi University, Nanning, ChinaGuangxi Laboratory on the Study of Coral Reefs in the South China Sea, Guangxi University, Nanning, ChinaSchool of Marine Sciences, Guangxi University, Nanning, ChinaCoral Reef Research Center of China, Guangxi University, Nanning, ChinaGuangxi Laboratory on the Study of Coral Reefs in the South China Sea, Guangxi University, Nanning, ChinaSchool of Marine Sciences, Guangxi University, Nanning, ChinaCoral Reef Research Center of China, Guangxi University, Nanning, ChinaGuangxi Laboratory on the Study of Coral Reefs in the South China Sea, Guangxi University, Nanning, ChinaSchool of Marine Sciences, Guangxi University, Nanning, ChinaSea urchins strongly affect reef ecology, and the bacteria associated with their gut digesta have not been well studied in coral reefs. In the current study, we analyze the bacterial composition of five sea urchin species collected from Luhuitou fringing reef, namely Stomopneustes variolaris, Diadema setosum, Echinothrix calamaris, Diadema savignyi, and Tripneustes gratilla, using high-throughput 16S rRNA gene-based pyrosequencing. Propionigenium, Prolixibacter, and Photobacterium were found to be the dominant bacterial genera in all five species. Interestingly, four sea urchin species, including S. variolaris, D. setosum, E. calamaris, and D. savignyi, displayed a higher mean total abundance of the three bacterial genera (69.72 ± 6.49%) than T. gratilla (43.37 ± 13.47%). Diversity analysis indicated that the gut digesta of sea urchin T. gratilla displayed a higher bacterial α-diversity compared with the other four species. PCoA showed that the four groups representing D. setosum, D. savignyi, E. calamaris, and S. variolaris were overlapping, but distant from the group representing T. gratilla. Predictive metagenomics performed by PICRUSt revealed that the abundances of genes involved in amino acid metabolism and metabolism of terpenoid and polyketide were higher in T. gratilla, while those involved in carbohydrate metabolism were higher in the other four sea urchin species. Therefore, our results indicated that the composition, diversity and predictive metabolic profiles of bacteria associated with the gut digesta of T. gratilla were significantly different from those of the other four sea urchin species in Luhuitou fringing reef.https://www.frontiersin.org/article/10.3389/fmicb.2019.01168/fullsea urchingut digesta bacteriacoral reefdegradationrestoration |
| spellingShingle | Qiucui Yao Qiucui Yao Qiucui Yao Qiucui Yao Qiucui Yao Kefu Yu Kefu Yu Kefu Yu Jiayuan Liang Jiayuan Liang Jiayuan Liang Yinghui Wang Yinghui Wang Yinghui Wang Baoqing Hu Xueyong Huang Xueyong Huang Xueyong Huang Biao Chen Biao Chen Biao Chen Zhenjun Qin Zhenjun Qin Zhenjun Qin The Composition, Diversity and Predictive Metabolic Profiles of Bacteria Associated With the Gut Digesta of Five Sea Urchins in Luhuitou Fringing Reef (Northern South China Sea) Frontiers in Microbiology sea urchin gut digesta bacteria coral reef degradation restoration |
| title | The Composition, Diversity and Predictive Metabolic Profiles of Bacteria Associated With the Gut Digesta of Five Sea Urchins in Luhuitou Fringing Reef (Northern South China Sea) |
| title_full | The Composition, Diversity and Predictive Metabolic Profiles of Bacteria Associated With the Gut Digesta of Five Sea Urchins in Luhuitou Fringing Reef (Northern South China Sea) |
| title_fullStr | The Composition, Diversity and Predictive Metabolic Profiles of Bacteria Associated With the Gut Digesta of Five Sea Urchins in Luhuitou Fringing Reef (Northern South China Sea) |
| title_full_unstemmed | The Composition, Diversity and Predictive Metabolic Profiles of Bacteria Associated With the Gut Digesta of Five Sea Urchins in Luhuitou Fringing Reef (Northern South China Sea) |
| title_short | The Composition, Diversity and Predictive Metabolic Profiles of Bacteria Associated With the Gut Digesta of Five Sea Urchins in Luhuitou Fringing Reef (Northern South China Sea) |
| title_sort | composition diversity and predictive metabolic profiles of bacteria associated with the gut digesta of five sea urchins in luhuitou fringing reef northern south china sea |
| topic | sea urchin gut digesta bacteria coral reef degradation restoration |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.01168/full |
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