Comparison of Disk Diffusion, E-Test, and Broth Microdilution Methods for Testing In Vitro Activity of Cefiderocol in <i>Acinetobacter baumannii</i>
The reference method for cefiderocol antimicrobial susceptibility testing is broth microdilution (BMD) with iron-depleted-Mueller-Hinton (ID-MH) medium, whereas breakpoints recommended for disk diffusion (DD) are based on MH-agar plates. We aimed to compare the performance of the commercial BMD test...
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MDPI AG
2023-07-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/7/1212 |
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| author | Natalia Kolesnik-Goldmann Helena M. B. Seth-Smith Klara Haldimann Frank Imkamp Tim Roloff Reinhard Zbinden Sven N. Hobbie Adrian Egli Stefano Mancini |
| author_facet | Natalia Kolesnik-Goldmann Helena M. B. Seth-Smith Klara Haldimann Frank Imkamp Tim Roloff Reinhard Zbinden Sven N. Hobbie Adrian Egli Stefano Mancini |
| author_sort | Natalia Kolesnik-Goldmann |
| collection | DOAJ |
| description | The reference method for cefiderocol antimicrobial susceptibility testing is broth microdilution (BMD) with iron-depleted-Mueller-Hinton (ID-MH) medium, whereas breakpoints recommended for disk diffusion (DD) are based on MH-agar plates. We aimed to compare the performance of the commercial BMD tests ComASP (Liofilchem) and UMIC (Bruker), and DD and E-test using MH- and ID-MH-agar plates with the reference BMD method using 100 carbapenem-resistant-<i>A. baumannii</i> isolates. Standard BMD was performed according to the EUCAST guidelines; DD and E-test were carried out using two commercial MH-agar plates (BioMérieux and Liofilchem) and an in-house ID-MH-agar plate, while ComASP and UMIC were performed according to the manufacturer’s guidelines. DD performed with the ID-MH-agar plates led to a higher categorical agreement (CA, 95.1%) with standard BMD and fewer categorization errors compared to the commercial MH-agar plates (CA BioMérieux 91.1%, Liofilchem 89.2%). E-test on ID-MH-agar plates exhibited a significantly higher essential agreement (EA, 75%) with standard BMD compared to the two MH-agar plates (EA BioMérieux 57%, Liofilchem 44%), and showed a higher performance in detecting high-level resistance than ComASP and UMIC (mean log2 difference with standard BMD for resistant isolates of 0.5, 2.83, and 2.08, respectively). In conclusion, DD and E-test on ID-MH-agar plates exhibit a higher diagnostic performance than on MH-agar plates and the commercial BMD methods. Therefore, we recommend using ID-MH-agar plates for cefiderocol susceptibility testing of <i>A. baumannii</i>. |
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| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-11T01:21:33Z |
| publishDate | 2023-07-01 |
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| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-637d09cd409b49d189ac2f982c3582b02023-11-18T18:03:51ZengMDPI AGAntibiotics2079-63822023-07-01127121210.3390/antibiotics12071212Comparison of Disk Diffusion, E-Test, and Broth Microdilution Methods for Testing In Vitro Activity of Cefiderocol in <i>Acinetobacter baumannii</i>Natalia Kolesnik-Goldmann0Helena M. B. Seth-Smith1Klara Haldimann2Frank Imkamp3Tim Roloff4Reinhard Zbinden5Sven N. Hobbie6Adrian Egli7Stefano Mancini8Institute of Medical Microbiology, University Zurich, Gloriastrasse 28/30, 8006 Zurich, SwitzerlandInstitute of Medical Microbiology, University Zurich, Gloriastrasse 28/30, 8006 Zurich, SwitzerlandInstitute of Medical Microbiology, University Zurich, Gloriastrasse 28/30, 8006 Zurich, SwitzerlandInstitute of Medical Microbiology, University Zurich, Gloriastrasse 28/30, 8006 Zurich, SwitzerlandInstitute of Medical Microbiology, University Zurich, Gloriastrasse 28/30, 8006 Zurich, SwitzerlandInstitute of Medical Microbiology, University Zurich, Gloriastrasse 28/30, 8006 Zurich, SwitzerlandInstitute of Medical Microbiology, University Zurich, Gloriastrasse 28/30, 8006 Zurich, SwitzerlandInstitute of Medical Microbiology, University Zurich, Gloriastrasse 28/30, 8006 Zurich, SwitzerlandInstitute of Medical Microbiology, University Zurich, Gloriastrasse 28/30, 8006 Zurich, SwitzerlandThe reference method for cefiderocol antimicrobial susceptibility testing is broth microdilution (BMD) with iron-depleted-Mueller-Hinton (ID-MH) medium, whereas breakpoints recommended for disk diffusion (DD) are based on MH-agar plates. We aimed to compare the performance of the commercial BMD tests ComASP (Liofilchem) and UMIC (Bruker), and DD and E-test using MH- and ID-MH-agar plates with the reference BMD method using 100 carbapenem-resistant-<i>A. baumannii</i> isolates. Standard BMD was performed according to the EUCAST guidelines; DD and E-test were carried out using two commercial MH-agar plates (BioMérieux and Liofilchem) and an in-house ID-MH-agar plate, while ComASP and UMIC were performed according to the manufacturer’s guidelines. DD performed with the ID-MH-agar plates led to a higher categorical agreement (CA, 95.1%) with standard BMD and fewer categorization errors compared to the commercial MH-agar plates (CA BioMérieux 91.1%, Liofilchem 89.2%). E-test on ID-MH-agar plates exhibited a significantly higher essential agreement (EA, 75%) with standard BMD compared to the two MH-agar plates (EA BioMérieux 57%, Liofilchem 44%), and showed a higher performance in detecting high-level resistance than ComASP and UMIC (mean log2 difference with standard BMD for resistant isolates of 0.5, 2.83, and 2.08, respectively). In conclusion, DD and E-test on ID-MH-agar plates exhibit a higher diagnostic performance than on MH-agar plates and the commercial BMD methods. Therefore, we recommend using ID-MH-agar plates for cefiderocol susceptibility testing of <i>A. baumannii</i>.https://www.mdpi.com/2079-6382/12/7/1212cefiderocol<i>A. baumannii</i>disk diffusionbroth microdilutionE-testiron-depletion |
| spellingShingle | Natalia Kolesnik-Goldmann Helena M. B. Seth-Smith Klara Haldimann Frank Imkamp Tim Roloff Reinhard Zbinden Sven N. Hobbie Adrian Egli Stefano Mancini Comparison of Disk Diffusion, E-Test, and Broth Microdilution Methods for Testing In Vitro Activity of Cefiderocol in <i>Acinetobacter baumannii</i> Antibiotics cefiderocol <i>A. baumannii</i> disk diffusion broth microdilution E-test iron-depletion |
| title | Comparison of Disk Diffusion, E-Test, and Broth Microdilution Methods for Testing In Vitro Activity of Cefiderocol in <i>Acinetobacter baumannii</i> |
| title_full | Comparison of Disk Diffusion, E-Test, and Broth Microdilution Methods for Testing In Vitro Activity of Cefiderocol in <i>Acinetobacter baumannii</i> |
| title_fullStr | Comparison of Disk Diffusion, E-Test, and Broth Microdilution Methods for Testing In Vitro Activity of Cefiderocol in <i>Acinetobacter baumannii</i> |
| title_full_unstemmed | Comparison of Disk Diffusion, E-Test, and Broth Microdilution Methods for Testing In Vitro Activity of Cefiderocol in <i>Acinetobacter baumannii</i> |
| title_short | Comparison of Disk Diffusion, E-Test, and Broth Microdilution Methods for Testing In Vitro Activity of Cefiderocol in <i>Acinetobacter baumannii</i> |
| title_sort | comparison of disk diffusion e test and broth microdilution methods for testing in vitro activity of cefiderocol in i acinetobacter baumannii i |
| topic | cefiderocol <i>A. baumannii</i> disk diffusion broth microdilution E-test iron-depletion |
| url | https://www.mdpi.com/2079-6382/12/7/1212 |
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