Development of a counterselectable seamless mutagenesis system in lactic acid bacteria

Development of a counterselectable seamless mutagenesis system in lactic acid bacteria

Abstract Background Lactic acid bacteria (LAB) are receiving more attention to act as cell factories for the production of high-value metabolites. However, the molecular tools for genetic modifying these strains are mainly vector-based double-crossover strategies, which are laborious and inefficient...

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Bibliographic Details
Main Authors: Yongping Xin, Tingting Guo, Yingli Mu, Jian Kong
Format: Article
Language:English
Published: BMC 2017-07-01
Series:Microbial Cell Factories
Subjects:
Lactic acid bacteria
Temperature-sensitive plasmid
Seamless mutagenesis
Counterselectable marker
pheS
Online Access:http://link.springer.com/article/10.1186/s12934-017-0731-8

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